A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme

Journal of Biotechnology

Volumn 137, Issue 1-4, 2008, Pages 1-7

  Kotera, Ippei ^a   Nagai, Takeharu ^b  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

Author keywords

DNA cloning; DNA manipulations; DNA recombination; High throughput; Type IIS restriction endonuclease

Indexed keywords

CATALYSTS; COSMIC RAYS; COST REDUCTION; DNA; ENZYME INHIBITION; ENZYMES; FOOD ADDITIVES; GENES; ORGANIC ACIDS; PROTEINS; THROUGHPUT; TUBES (COMPONENTS);

DNA CLONING; DNA FRAGMENTS; DNA LIGASE; DNA MANIPULATIONS; DNA POLYMERASES; DNA RECOMBINATION; DNA-POLYMERASE INHIBITOR; HIGH THROUGHPUTS; HIGH-THROUGHPUT; HIGHLY EFFICIENT; PCR PRODUCTS; REACTION SOLUTIONS; RECOMBINATION REACTIONS; RESTRICTION ENDONUCLEASE; RESTRICTION ENZYMES; SELECTIVE DIGESTION; SIGNIFICANT REDUCTION; SINGLE-STEP; TYPE IIS RESTRICTION ENDONUCLEASE;

NUCLEIC ACIDS;

APHIDICOLIN; CYAN FLUORESCENT PROTEIN; DNA FRAGMENT; GREEN FLUORESCENT PROTEIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RESTRICTION ENDONUCLEASE; YELLOW FLUORESCENT PROTEIN;

ARTICLE; DELETION MUTANT; DNA RECOMBINATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC MANIPULATION; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PYROCOCCUS;

APHIDICOLIN; CLONING, MOLECULAR; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; DNA LIGASES; DNA-DIRECTED DNA POLYMERASE; ENZYME INHIBITORS; POLYMERASE CHAIN REACTION; SPECTROMETRY, FLUORESCENCE;

EID: 52949099479     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.07.1816     Document Type: Article

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