Hyperthermia alters the interaction of proteins of the Mre11 complex in irradiated cells

Cytometry Part A

Volumn 77, Issue 10, 2010, Pages 940-952

  Gerashchenko, Bogdan I ^a,b   Gooding, Gerirose ^a   Dynlacht, Joseph R ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b R E KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY   (Ukraine)

Author keywords

Colocalization; DNA double strand breaks; DNA repair foci; Hyperthermia; Immunofluorescence; Ionizing radiation; Mre11 complex

Indexed keywords

DNA BINDING PROTEIN; MRE11A PROTEIN, HUMAN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RAD50 PROTEIN, HUMAN;

ARTICLE; CELL NUCLEUS; CONFOCAL MICROSCOPY; DNA REPAIR; DOUBLE STRANDED DNA BREAK; HEAT; HUMAN; KINETICS; METABOLISM; PHYSIOLOGY; RADIATION DOSE; RADIATION EXPOSURE; TUMOR CELL LINE;

CELL LINE, TUMOR; CELL NUCLEUS; DNA BREAKS, DOUBLE-STRANDED; DNA REPAIR; DNA REPAIR ENZYMES; DNA-BINDING PROTEINS; HOT TEMPERATURE; HUMANS; KINETICS; MICROSCOPY, CONFOCAL; RADIATION TOLERANCE;

EID: 77957278983     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20955     Document Type: Article

References (43)

1. Ben-Hur E, Elkind MM, Bronk BV. Thermally enhanced radioresponse of cultured Chinese hamster cells: Inhibition of repair of sublethal damage and enhancement of lethal damage. Radiat Res 1974;58:38-51.
2. Dewey WC, Hopwood LE, Sapareto LA, Gerweck LE. Cellular responses to combinations of hyperthermia and radiation. Radiology 1977;123:463-474.
3. Lepock JR, Cheng KH, Al-Qysi H, Kruuv J. Thermotropic lipid and protein transitions in Chinese hamster lung cell membranes: Relationship to hyperthermic cell killing. Can J Biochem 1983;61:421-427.
4. Lepock JR, Frey HE, Rodahl AM, Kruuv J. Thermal analysis of CHL V79 cells using differential scanning calorimetry: Implications for hyperthermic cell killing and the heat shock response. J Cell Physiol 1988;137:14-24.
5. Freeman ML, Borrelli MJ, Meredith MJ, Lepock JR. On the path to the heat shock response: Destabilization and formation of partially folded protein intermediates, a consequence of protein thiol modification. Free Radic Biol Med 1999;26:737-745.
6. Corry PM, Robinson S, Getz S. Hyperthermic effects on DNA repair mechanisms. Radiology 1977;123:475-482.
7. Dewey WC, Sapareto SA, Betten DA. Hyperthermic radiosensitization of synchronous Chinese hamster cells: Relationship between lethality and chromosomal aberrations. Radiat Res 1978;76:48-59.
8. Mills MD, Meyn RE. Effects of hyperthermia on DNA repair mechanisms. Radiat Res 1981;87:314-328.
9. Radford IR. Effects of hyperthermia on the repair of X-ray induced DNA double strand breaks in mouse L cells. Int J Radiat Biol 1983;5:551-557.
10. Dikomey E, Franzke J. Effect of heat on induction and repair of DNA strand breaks in X-irradiated CHO cells. Int J Radiat Biol 1992;61:221-233.
11. Wong RSL, Dynlacht J, Cedervall B, Dewey WC. Analysis by pulsed field gel electrophoresis of DNA double-strand breaks induced by heat and/or X-irradiation in bulk and replicating DNA of CHO cells. Int J Radiat Biol 1995;68:141-152.
12. Khanna KK, Jackson SP. DNA double-strand breaks: Signaling, repair and the cancer connection. Nat Genet 2001;27:247-254.
13. Thompson LH, Schild D. Recombinational DNA repair and human disease. Mutat Res 2002;509:49-78.
14. Kampinga HH, Dynlacht JR, Dikomey E. Mechanism of radiosensitization by hyperthermia (≥ 43°C) as derived from studies with DNA repair defective mutant cell lines. Int J Hyperthermia 2004;20:131-139.
15. Tauchi H, Kobayashi J, Morishima K, van Gent DC, Shiraishi T, Verkaik NS, van-Heems D, Ito E, Nakamura A, Sonoda E, Takata M, Takeda S, Matsuura S, Komatsu K. Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells. Nature 2002;420:93-98.
16. Zhu WG, Seno J, Beck BD, Dynlacht JR. Translocation of Mre11 from the nucleus to the cytoplasm as a mechanism of radiosensitization by heat. Radiat Res 2001;156:92-102.
17. Seno DJ, Dynlacht JR. Intracellular redistribution and modification of proteins of the Mre11/Rad50/Nbs1 DNA repair complex following irradiation and heat-shock. J Cell Physiol 2004;199:157-170.
18. Williams RS, Williams JS, Tainer JA. Mre11-Rad50-Nbs1 is a keystone complex connecting DNA repair machinery, double-strand break signaling, and the chromatin template. Biochem Cell Biol 2007;85:509-520.
19. Dolganov GM, Maser RS, Novikov A, Tosto L, Chong S, Bressan DA, Petrini JHJ. Human Rad50 is physically associated with human Mre11: Identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol Cell Biol 1996;16:4832-4841.
20. Lee J-H, Ghirlando R, Bhaskara V, Hoffmeyer MR, Gu J, Paull TT. Regulation of Mre11/Rad50 by Nbs1: Effects on nucleotide-dependent DNA binding and association with ataxia-telangiectasia-like disorder mutant complexes. J Biol Chem 2003;278:45171-45181.
21. Luo G, Yao MS, Bender CF, Mills M, Bladl AR, Bradley A, Petrini JHJ. Disruption of mRad50 causes embryonic stem cell lethality, abnormal embryonic development, and sensitivity to ionizing radiation. Proc Natl Acad Sci USA 1999;96:7376-7381.
22. Yamaguchi-Iwai Y, Sonoda E, Sasaki MS, Morrison C, Haraguchi T, Hiraoka Y, Yamashita YM, Yagi T, Takata M, Price C, Kakazu N, Takeda S. Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells. EMBO J 1999;18:6619-6629.
23. Desai-Mehta A, Cerosaletti KM, Concannon P. Distinct functional domains of nibrin mediate Mre11 binding, focus formation, and nuclear localization. Mol Cell Biol 2001;21:2184-2191.
24. Maser RS, Monsen KJ, Nelms BE, Petrini JHJ. hMre11 and hRad50 nuclear foci are induced during the normal cellular response to DNA double-strand breaks. Mol Cell Biol 1997;17:6087-6096.
25. Carney JP, Maser RS, Olivares H, Davis EM, Le Beau M, Yates JR 3rd, Hays L, Morgan WF, Petrini JHJ. The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: Linkage of double-strand break repair to the cellular DNA damage response. Cell 1998;93:477-486.
26. Digweed M, Demuth I, Rothe S, Scholz R, Jordan A, Grötzinger C, Schindler D, Grompe M, Sperling K. SV40 large T-antigen disturbs the formation of nuclear DNA-repair foci containing MRE11. Oncogene 2002;21:4873-4878.
27. van Veelen LR, Cervelli T, van de Rakt MWMM, Theil AF, Essers J, Kanaar R. Analysis of ionizing radiation-induced foci of DNA damage repair proteins. Mutat Res 2005;574:22-33.
28. Gerashchenko BI, Dynlacht JR. A tool for enhancement and scoring of DNA repair foci. Cytometry Part A 2009;75A:245-252.
29. 125IdU-induced DNA double-strand breaks with γ-H2AX antibody. Radiat Res 2002;158:486-492.
30. Scully R, Chen J, Ochs RL, Keegan K, Hoekstra M, Feunteun J, Livingston DM. Dynamic changes of BRCA1 subnuclear location and phosphorylation state are initiated by DNA damage. Cell 1997;90:425-435.
31. Rasband WS. ImageJ. Bethesda, MD: U.S. National Institute of Health; 1997-2007. Available at: http://rsb.info.nih.gov/ij/.
32. Manders EMM, Verbeek FJ, Aten JA. Measurement of co-localization of objects in dual-colour confocal images. J Microsc 1993;169:375-382.
33. 0, and N-type calcium channel complex at a presynaptic nerve terminal: Analysis by quantitative immunocolocalization. J Neurosci 2004;24:4070-4081.
34. Nevaldine B, Longo JA, King GA, Vilenchik M, Sagerman RH, Hahn PJ. Induction and repair of DNA double-strand breaks. Radiat Res 1993;133:370-374.
35. Löbrich M, Rydberg B, Cooper PK. Repair of X-ray-induced DNA double-strand breaks in specific Not I restriction fragments in human fibroblasts: Joining of correct and incorrect ends. Proc Natl Acad Sci USA 1995;92:12050-12054.
36. Dikomey E. Effect of hyperthermia at 42 and 45°C on repair of radiation induced DNA strand breaks in CHO cells. Int J Radiat Biol Relat Stud Phys Chem Med 1982;41:603-614.
37. Warters RL, Axtell J. Repair of DNA strand breaks at hyperthermic temperatures in Chinese hamster ovary cells. Int J Radiat Biol 1992;61:43-48.
38. Stege GJJ, Li GC, Li L, Kampinga HH, Konings AW. On the role of hsp72 in heat-induced intranuclear protein aggregation. Int J Hyperthermia 1994;10:659-674.
39. Stege GJJ, Kampinga HH, Konings AW. Heat-induced intranuclear protein aggregation and thermal radiosensitization. Int J Radiat Biol 1995;67:203-209.
40. Dynlacht JR, Xu M, Pandita RK, Wetzel EA, Roti Roti JL. Effects of heat shock on the Mre11/Rad50/Nbs1 complex in irradiated or unirradiated cells. Int J Hyperthermia 2004;20:144-156.
41. Xu M, Myerson RJ, Straube WL, Moros EG, Lagroye I, Wang LL, Lee JT, Roti Roti JL. Radiosensitization of heat resistant human tumour cells by 1 hour at 41.1°C and its effect on DNA repair. Int J Hyperthermia 2002;18:385-403.
42. Beck BD, Dynlacht JR. Heat-induced aggregation of XRCC5 (Ku80) in non-tolerant and thermotolerant cells. Radiat Res 2001;156:767-774.
43. Laszlo A, Davidson T, Harvey A, Sim JE, Malyapa RS, Spitz DR, Roti Roti JL. Alterations in heat-induced radiosensitization accompanied by nuclear structure alterations in Chinese hamster cells. Int J Hyperthermia 2006;22:43-60.