Frontiers of microscopy-based research into cell-matrix adhesions

Current Opinion in Cell Biology

Volumn 22, Issue 5, 2010, Pages 659-668

  Medalia, Ohad ^a   Geiger, Benjamin ^b  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; ALPHA ACTININ; BETA3 INTEGRIN; FILAMIN; INTEGRIN; MYOSIN II; PAXILLIN; TROPOMYOSIN;

CELL ADHESION; CELL CULTURE; CELL INTERACTION; CELL MIGRATION; CELL STRUCTURE; CELLULAR DISTRIBUTION; CRYOELECTRON MICROSCOPY; CYTOARCHITECTURE; ELECTRON MICROSCOPY; ELECTRON TOMOGRAPHY; EXTRACELLULAR MATRIX; FLUORESCENCE MICROSCOPY; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; FOCAL ADHESION; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; METHODOLOGY; MICROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVIEW; TISSUE CHARACTERIZATION; TRANSMISSION ELECTRON MICROSCOPY; X RAY DIFFRACTION;

ANIMALS; CELL-MATRIX JUNCTIONS; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; MYOCYTES, SMOOTH MUSCLE; RESEARCH DESIGN;

EID: 77957237697     PISSN: 09550674     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ceb.2010.08.006     Document Type: Review

References (58)

1. Heaysman J.E., Pegrum S.M. Early contacts between fibroblasts. An ultrastructural study. Exp Cell Res 1973, 78:71-78.
2. Abercrombie M., Heaysman J.E., Pegrum S.M. The locomotion of fibroblasts in culture. I. Movements of the leading edge. Exp Cell Res 1970, 59:393-398.
3. Dunn G., Jones G. Michael Abercrombie: the pioneer ethologist of cells. Trends Cell Biol 1998, 8:124-126.
4. Weston J.A. In memory of Michael Abercrombie. Prog Clin Biol Res 1982, 85(Pt B):1-4.
5. Singer I.I. The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5nm microfilaments in hamster and human fibroblasts. Cell 1979, 16:675-685.
6. Chen W.T. Mechanism of retraction of the trailing edge during fibroblast movement. J Cell Biol 1981, 90:187-200.
7. Lazarides E., Weber K. Actin antibody: the specific visualization of actin filaments in non-muscle cells. Proc Natl Acad Sci U S A 1974, 71:2268-2272.
8. Goldman R.D., Lazarides E., Pollack R., Weber K. The distribution of actin in non-muscle cells. The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells. Exp Cell Res 1975, 90:333-344.
9. Lazarides E. Actin, alpha-actinin, and tropomyosin interaction in the structural organization of actin filaments in nonmuscle cells. J Cell Biol 1976, 68:202-219.
10. Lazarides E., Burridge K. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells. Cell 1975, 6:289-298.
11. Weber K., Lazarides E., Goldman R.D., Vogel A., Pollack R. Localization and distribution of actin fibers in normal transformed and revertant cells. Cold Spring Harb Symp Quant Biol 1975, 39(Pt 1):363-369.
12. Lazarides E. Intermediate filaments-chemical heterogeneity in differentiation. Cell 1981, 23:649-650.
13. Lazarides E. Intermediate filaments: a chemically heterogeneous, developmentally regulated class of proteins. Annu Rev Biochem 1982, 51:219-250.
14. Geiger B. A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells. Cell 1979, 18:193-205.
15. Burridge K., Feramisco J.R. Microinjection and localization of a 130K protein in living fibroblasts: a relationship to actin and fibronectin. Cell 1980, 19:587-595.
16. Burridge K., Connell L. Talin: a cytoskeletal component concentrated in adhesion plaques and other sites of actin-membrane interaction. Cell Motil 1983, 3:405-417.
17. Mangeat P., Burridge K. Actin-membrane interaction in fibroblasts: what proteins are involved in this association?. J Cell Biol 1984, 99:95s-103s.
18. Turner C.E., Glenney J.R., Burridge K. Paxillin: a new vinculin-binding protein present in focal adhesions. J Cell Biol 1990, 111:1059-1068.
19. Geiger B., Avnur Z., Kreis T.E., Schlessinger J. The dynamics of cytoskeletal organization in areas of cell contact. Cell Muscle Motil 1984, 5:195-234.
20. Zamir E., Geiger B., Kam Z. Quantitative multicolor compositional imaging resolves molecular domains in cell-matrix adhesions. PLoS One 2008, 3:e1901.
21. Geiger B., Dutton A.H., Tokuyasu K.T., Singer S.J. Immunoelectron microscope studies of membrane-microfilament interactions: distributions of alpha-actinin, tropomyosin, and vinculin in intestinal epithelial brush border and chicken gizzard smooth muscle cells. J Cell Biol 1981, 91:614-628.
22. Geiger B., Tokuyasu K.T., Dutton A.H., Singer S.J. Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes. Proc Natl Acad Sci U S A 1980, 77:4127-4131.
23. Zaidel-Bar R., Geiger B. The switchable integrin adhesome. J Cell Sci 2010, 123:1385-1388.
24. Zamir E., Geiger B. Molecular complexity and dynamics of cell-matrix adhesions. J Cell Sci 2001, 114:3583-3590.
25. Zaidel-Bar R., Itzkovitz S., Ma'ayan A., Iyengar R., Geiger B. Functional atlas of the integrin adhesome. Nat Cell Biol 2007, 9:858-867.
26. Sabanay I., Arad T., Weiner S., Geiger B. Study of vitrified, unstained frozen tissue sections by cryoimmunoelectron microscopy. J Cell Sci 1991, 100(Pt 1):227-236.
27. Ben-Harush K., Maimon T., Patla I., Villa E., Medalia O. Visualizing cellular processes at the molecular level by cryo-electron tomography. J Cell Sci 2010, 123:7-12.
28. Medalia O., Weber I., Frangakis A.S., Nicastro D., Gerisch G., Baumeister W. Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 2002, 298:1209-1213.
29. Lucic V., Forster F., Baumeister W. Structural studies by electron tomography: from cells to molecules. Annu Rev Biochem 2005, 74:833-865.
30. Dubochet J., Adrian M., Chang J.J., Homo J.C., Lepault J., McDowall A.W., Schultz P. Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 1988, 21:129-228.
31. Frank J. Introduction: principles of electron tomography. Electron Tomography 1992, 1-13. Plenum Press. J. Frank (Ed.).
32. Radermacher M. Weighted back-projection methods. Electron Tomography 1992, 91-115. Plenum Press. J. Frank (Ed.).
33. Koster A.J., Grimm R., Typke D., Hegerl R., Stoschek A., Walz J., Baumeister W. Perspectives of molecular and cellular electron tomography. J Struct Biol 1997, 120:276-308.
34. Frank J., Wagenknecht T., McEwen B.F., Marko M., Hsieh C.E., Mannella C.A. Three-dimensional imaging of biological complexity. J Struct Biol 2002, 138:85-91.
35. Dierksen K., Typke D., Hegerl R., Baumeister W. Towards automatic electron tomography. 2. Implementation of autofocus and low-dose procedures. Ultramicroscopy 1993, 49:109-120.
36. Dierksen K., Typke D., Hegerl R., Koster A.J., Baumeister W. Towards automatic electron tomography. Ultramicroscopy 1992, 40:71-87.
37. Grimm R., Koster A.J., Ziese U., Typke D., Baumeister W. Zero-loss energy filtering under low-dose conditions using a post-column energy filter. J Microsc Oxford 1996, 183:60-68.
38. Al-Amoudi A., Chang J.J., Leforestier A., McDowall A., Salamin L.M., Norlen L.P., Richter K., Blanc N.S., Studer D., Dubochet J. Cryo-electron microscopy of vitreous sections. EMBO J 2004, 23:3583-3588.
39. Al-Amoudi A., Diez D.C., Betts M.J., Frangakis A.S. The molecular architecture of cadherins in native epidermal desmosomes. Nature 2007, 450:832-837.
40. Castano-Diez D., Al-Amoudi A., Glynn A.M., Seybert A., Frangakis A.S. Fiducial-less alignment of cryo-sections. J Struct Biol 2007, 159:413-423.
41. Gruska M., Medalia O., Baumeister W., Leis A. Electron tomography of vitreous sections from cultured mammalian cells. J Struct Biol 2008, 161:384-392.
42. Hsieh C.E., Marko M., Frank J., Mannella C.A. Electron tomographic analysis of frozen-hydrated tissue sections. J Struct Biol 2002, 138:63-73.
43. Salje J., Zuber B., Lowe J. Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation. Science 2009, 323:509-512.
44. Al-Amoudi A., Studer D., Dubochet J. Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. J Struct Biol 2005, 150:109-121.
45. Brown S., Levinson W., Spudich J.A. Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction. J Supramol Struct 1976, 5:119-130.
46. Small J.V., Herzog M., Haner M., Abei U. Visualization of actin filaments in keratocyte lamellipodia: negative staining compared with freeze-drying. J Struct Biol 1994, 113:135-141.
47. Svitkina T.M., Verkhovsky A.B., McQuade K.M., Borisy G.G. Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation. J Cell Biol 1997, 139:397-415.
48. Heuser J.E., Kirschner M.W. Filament organization revealed in platinum replicas of freeze-dried cytoskeletons. J Cell Biol 1980, 86:212-234.
49. Bretschneider T., Diez S., Anderson K., Heuser J., Clarke M., Muller-Taubenberger A., Kohler J., Gerisch G. Dynamic actin patterns and Arp2/3 assembly at the substrate-attached surface of motile cells. Curr Biol 2004, 14:1-10.
50. Wolfenson H., Lubelski A., Regev T., Klafter J., Henis Y.I., Geiger B. A role for the juxtamembrane cytoplasm in the molecular dynamics of focal adhesions. PLoS One 2009, 4:e4304.
51. Digman M.A., Brown C.M., Horwitz A.R., Mantulin W.W., Gratton E. Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy. Biophys J 2008, 94:2819-2831.
52. Brown C.M., Dalal R.B., Hebert B., Digman M.A., Horwitz A.R., Gratton E. Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope. J Microsc 2008, 229:78-91.
53. Medalia O., Beck M., Ecke M., Weber I., Neujahr R., Baumeister W., Gerisch G. Organization of actin networks in intact filopodia. Curr Biol 2007, 17:79-84.
54. Urban E., Jacob S., Nemethova M., Resch G.P., Small J.V. Electron tomography reveals unbranched networks of actin filaments in lamellipodia. Nat Cell Biol 2010, 12:429-435.
55. Sartori A., Gatz R., Beck F., Rigort A., Baumeister W., Plitzko J.M. Correlative microscopy: bridging the gap between fluorescence light microscopy and cryo-electron tomography. J Struct Biol 2007, 160:135-145.
56. Schwartz C.L., Sarbash V.I., Ataullakhanov F.I., McIntosh J.R., Nicastro D. Cryo-fluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching. J Microsc 2007, 227:98-109.
57. Plitzko J.M., Rigort A., Leis A. Correlative cryo-light microscopy and cryo-electron tomography: from cellular territories to molecular landscapes. Curr Opin Biotechnol 2009, 20:83-89.
58. Hein B., Willig K.I., Hell S.W. Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell. Proc Natl Acad Sci U S A 2008, 105:14271-14276.