Electron tomography of vitreous sections from cultured mammalian cells

Journal of Structural Biology

Volumn 161, Issue 3, 2008, Pages 384-392

  Gruska, Manuela ^a   Medalia, Ohad ^a,b   Baumeister, Wolfgang ^a   Leis, Andrew ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

ATP synthase F1F0 dimers; Colloidal gold; Correlative microscopy; Cryo electron tomography; Cryo fluorescence microscopy; Cryosections; HL 1 cardiomyocytes; Mammalian mitochondria; P19 embryonic stem cells; Vitreous sections

Indexed keywords

COLLOIDAL GOLD; FLUORESCENT DYE; QUANTUM DOT;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; CONTROLLED STUDY; ELECTRON BEAM TOMOGRAPHY; FLUORESCENCE MICROSCOPY; FREEZING; HYPERBARISM; MAMMAL CELL; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE; TOMOGRAPHY; ULTRAMICROTOMY; VITREOUS BODY;

ANIMALS; CELL LINE; CRYOELECTRON MICROSCOPY; CRYOULTRAMICROTOMY; GOLD COLLOID; MICE; MICROSCOPY, FLUORESCENCE; MITOCHONDRIAL PROTON-TRANSLOCATING ATPASES; MYOCYTES, CARDIAC; TOMOGRAPHY;

EUKARYOTA; MAMMALIA;

EID: 40649100773     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2007.10.008     Document Type: Article

References (34)

1. Afzelius B.A., and Maunsbach A.B. Biological ultrastructure research; the first 50 years. Tissue Cell 36 (2004) 83-94
2. Balmes O.J.-O.M., Karlsson G., and Bovin J.-O. Cryo-TEM observation of 3-dimensionally ordered aggregates of 5-nm gold particles in organic solvents. J. Nanoparticle Res. 6 (2004) 569-576
3. Baumeister W. From proteomic inventory to architecture. FEBS Lett. 579 (2005) 933-937
4. Bouchet-Marquis C., Dubochet J., and Fakan S. Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture. Histochem. Cell Biol. 125 (2006) 43-51
5. Chretien, D., Kenney, J.M., Fuller, S.D., Wade, R.H., 1996. Determination of microtubule polarity by cryo-electron microscopy. 4, 1031-1040.
6. Claycomb W.C., Lanson L.A.J., Stallworth B.S., Egeland D.B., Delcarpio J.B., Bahinski A., and Izzo N.J.J. HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte. Proc. Natl. Acad. Sci. USA 95 (1998) 2979-2984
7. Dubochet J., and McDowall A.W. Vitrification of pure water for electron microscopy. J. Microsc. 124 (1981) RP3-RP4
8. Dubochet J., and Sartori Blanc N. The cell in absence of aggregation artifacts. Micron 32 (2001) 91-99
9. Dudkina N.V., Heinemeyer J., Keegstra W., Boekema E.J., and Braun H.-P. Structure of dimeric ATP synthase from mitochondria: An angular association of monomers induces the strong curvature of the inner membrane. FEBS Lett. 579 (2005) 5769-5772
10. Frangakis A.S., Stoschek A., and Hegerl R. Wavelet transform filtering and nonlinear anisotropic diffusion assessed for signal reconstruction performance on multidimensional biomedical data. IEEE Trans. Biomed. Eng. 48 (2001) 213-222
11. Garvalov B.K., Zuber B., Bouchet-Marquis C., Kudryashev M., Gruska M., Beck M., Leis A., Frischknecht F., Bradke F., Baumeister W., Dubochet J., and Cyrklaff M. Luminal particles within cellular microtubules. J. Cell Biol. 174 (2006) 759-765
12. Hegerl R. The EM program package: A platform for image processing in biological electron microscopy. J. Struct. Biol. 116 (1996) 30-34
13. Hiramatsu H., and Osterloh F.E. A simple large-scale synthesis of nearly monodisperse gold and silver nanoparticles with adjustable sizes and with exchangeable surfactants. Chem. Mat. 16 (2004) 2509-2511
14. Hsieh C.E., Leith A., Mannella C.A., Frank J., and Marko M. Towards high-resolution three-dimensional imaging of native mammalian tissue: electron tomography of frozen-hydrated rat liver sections. J. Struct. Biol. 153 (2006) 1-13
15. Hsieh C.E., Marko M., Frank J., and Mannella C.A. Electron tomographic analysis of frozen-hydrated tissue sections. J. Struct. Biol. 138 (2002) 63-73
16. Jones-Villeneuve E.M., McBurney M.W., Rogers K.A., and Kalnins V.I. Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. J. Cell Biol. 94 (1982) 253-262
17. Leis A., Andrees L., Gruska M., Al-Amoudi A., Sartori A., Dubochet J., and Baumeister W. Cryo-electron tomography and fluorescence microscopy of unicellular algae in vitreous cryosections. Microsc. Microanal. 11 (2005) 330CD
18. Mannella C.A. Structure and dynamics of the mitochondrial inner membrane cristae 1763 (2006) 542-548
19. Marko M., Hsieh C., Schalek R., Frank J., and Mannella C. Focused-ion-beam thinning of frozen-hydrated biological specimens for cryoelectron microscopy. Nat. Methods 4 (2007) 215-217
20. Masich S., Östberg T., Norlén L., Shupliakov O., and Daneholt B. A procedure to deposit fiducial markers on vitreous cryo-sections for cellular tomography. J. Struct. Biol. 156 (2006) 461-468
21. McBurney M.W., Jones-Villeneuve E.M., Edwards M.K., and Anderson P.J. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature 299 (1982) 165-167
22. Medalia O., Weber I., Frangakis A.S., Nicastro D., Gerisch G., and Baumeister W. Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 298 (2002) 1209-1213
23. Mulders H. The use of a SEM/FIB dual beam applied to biological samples. GIT Imaging Microsc. 2 (2003) 8-10
24. Nicastro D., Frangakis A.S., Typke D., and Baumeister W. Cryo-electron tomography of Neurospora mitochondria. J. Struct. Biol. 129 (2000) 48-56
25. Nickell S., Förster F., Linaroudis A., Del Net W., Beck F., Hegerl R., Baumeister W., and Plitzko J.M. TOM software toolbox: acquisition and analysis for electron tomography. J. Struct. Biol. 149 (2005) 227-234
26. Nickell S., Kofler C., Leis A., and Baumeister W. A visual approach to proteomics. Nature Rev. Mol. Cell Biol. 7 (2006) 225-230
27. Ortiz J., Förster F., Kürner J., Linaroudis A., and Baumeister W. Mapping 70S ribosomes in intact cells by cryoelectron tomography and pattern recognition. J. Struct. Biol. 156 (2006) 334-341
28. Pelloux S., Robillard J., Ferrera R., Bilbaut A., Ojeda C., Saks V., Ovize M., and Tourneur Y. Non-beating HL-1 cells for confocal microscopy: application to mitochondrial functions during cardiac preconditioning. Prog. Biophys. Mol. Biol. 90 (2006) 270-298
29. Sartori A., Gatz R., Beck F., Rigort A., Baumeister W., and Plitzko J.M.P. Correlative microscopy: bridging the gap between fluorescence light microscopy and cryo-electron tomography. J. Struct. Biol. 160 (2007) 135-145
30. Sartori N., Richter K., and Dubochet J. Vitrification depth can be increased more than 10-fold by high-pressure freezing. J. Microsc. 172 (1993) 55-61
31. Studer D., and Gnaegi H. Minimal compression of ultrathin sections with use of an oscillating diamond knife. J. Microsc. 197 (2000) 94-100
32. Studer D., Graber W., Al-Amoudi A., and Eggli P. A new approach for cryofixation by high-pressure freezing. J. Microsc. 203 (2001) 285-294
33. Tokuyasu K.T. Immunochemistry on ultrathin frozen-sections. Histochem. J. 12 (1980) 381-403
34. Zuber B., Nikonenko I., Klauser P., Muller D., and Dubochet J. The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes. Proc. Natl. Acad. Sci. USA 102 (2005) 19192-19197