A high-throughput differential filtration assay to screen and select detergents for membrane proteins

Analytical Biochemistry

Volumn 407, Issue 1, 2010, Pages 1-11

  Vergis, James M ^a   Purdy, Michael D ^a   Wiener, Michael C ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

High throughput assay; Membrane protein detergent stability; Precrystallization screening; Structural biology

Indexed keywords

CRYSTALS; MEMBRANES; MICROFILTRATION; PURIFICATION; SOAPS (DETERGENTS);

CRITICAL STEPS; HIGH THROUGHPUT; HIGH-THROUGHPUT ASSAYS; INTEGRAL MEMBRANE PROTEINS; MEMBRANE PROTEINS; PURIFIED PROTEIN; STRUCTURAL BIOLOGY; STRUCTURAL STUDIES;

PROTEINS;

DETERGENT; MEMBRANE PROTEIN;

ARTICLE; ASSAY; FILTRATION; GEL FILTRATION CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; PRIORITY JOURNAL; PROTEIN STABILITY; SOLUBILITY;

EID: 77956874534     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.07.019     Document Type: Article

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