Validation of a self-excising marker in the human pathogen aspergillus fumigatus by employing the βrec/six site-specific recombination system

Applied and Environmental Microbiology

Volumn 76, Issue 18, 2010, Pages 6313-6317

  Hartmann, Thomas ^a   Dümig, Michaela ^a   Jaber, Basem M ^c   Szewczyk, Edyta ^a   Olbermann, Patrick ^a   Morschhäuser, Joachim ^b   Krappmann, Sven ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c UNIVERSITY OF JORDAN   (Jordan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS FUMIGATUS; FILAMENTOUS FUNGI; GENE REPLACEMENT; GENE TARGETING; HUMAN PATHOGENS; RECOGNITION SEQUENCE; RECOMBINATION SYSTEM; SERINE RECOMBINASE; SITE-SPECIFIC RECOMBINATION;

AMINO ACIDS; GENETIC ENGINEERING;

PHOSPHATASES;

DNA POLYMERASE; OLIGONUCLEOTIDE; SITE SPECIFIC RECOMBINASE; SITE-SPECIFIC RECOMBINASE;

AMINO ACID; BACTERIUM; FUNGAL DISEASE; GENE EXPRESSION; GENETIC MARKER; HOMINID; PATHOGEN; PUBLIC HEALTH; RECOMBINATION;

ARTICLE; ASPERGILLUS FUMIGATUS; EVALUATION; GENE TARGETING; GENETIC ENGINEERING; GENETIC MARKER; GENETIC RECOMBINATION; GENETICS; METABOLISM; METHODOLOGY;

ASPERGILLUS FUMIGATUS; DNA NUCLEOTIDYLTRANSFERASES; GENE TARGETING; GENETIC ENGINEERING; GENETIC MARKERS; OLIGONUCLEOTIDES; RECOMBINATION, GENETIC;

ASPERGILLUS FUMIGATUS; BACTERIA (MICROORGANISMS); FUNGI;

EID: 77956825249     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00882-10     Document Type: Article

References (46)

1. Alonso, J. C., C. Gutierrez, and F. Rojo. 199S. The role of chromatinassociated protein Hbsu in beta-mediated DNA recombination is to facilitate the joining of distant recombination sites. Mol. Microbiol. 18:471-478.
2. Alonso, J. C., F. Weise, and F. Rojo. 1995. The Bacillus subtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the ß recombinase of plasmid pSM.19035. J. Biol. Chem. 270:2938-2945.
3. Bayram, Ö., S. Krappmann, M. Ni, J. W. Bok, K. Helmstaedt, O. Valerius, S. Braus-Stromeyer, N. J. Kwon, N. P. Keller, J. H. Yu, and G. H. Braus. 2008. VelB/VeA/LaeA complex coordinates light signal with fungal development and secondary metabolism. Science 320:1.504-1506.
4. Canosa, I., F. Rojo, and J. C. Alonso. 1996. Site-specific recombination by the ß protein from the streptococcal plasmid pSM.1.9035: minimal, recombination, sequences and crossing over site. Nucleic Acids Res. 24:2712-2717.
5. KU80 mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus. Eukaryot. Cell 5:207-211.
6. d'Enf'ert, C. 1996. Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5'-decarboxylase gene, pyrG, as a unique transformation marker. Curr. Genet. 30:76-82.
7. Diaz, V., F. Rojo, A. C. Martinez, J. C. Alonso, and A. Bernad. 1999. The prokaryotic β-recomhinase catalyzes site-specific recombination in mammalian cells. J. Biol. Chem. 274:6634-6640.
8. Diaz, V., P. Servert, I. Prieto, M. A. Gonzalez, A. C. Martinez, J. C. Alonso, and A. Bemad. 2001. New insights into host factor requirements for prokaryotic β-recombinase-mediated reactions in mammalian cells. J. Biol. Chem. 276:1.6257-16264.
9. Forment, J. V., D. Ramon, and A. P. MacCabe. 2006. Consecutive gene deletions in Aspergillus nidulans: application of the CreZiotP system. Curr. Genet. 50:217-224.
10. Gao, M., and M. Teplitski. 2008. RIVET-a tool for in vivo analysis of symbiotically relevant gene expression in Sinorhizobium metiloti. Mol. Plant Microbe Interact. 21:162-170.
11. Gooch, V. D., A. Mehra, L. F. Larrondo, J. Fox, M. Touroutoutoudls, J. J. Loros, and J. C. Dunlap. 2008. Fully codon-optimized luciferase uncovers novel temperature characteristics of the Neurospora clock. Eukaryot. Cell 7:28-37.
12. Grønlund, J. T., C. Stemmer, J. Lichota, T. Merkle, and K. D. Grasser. 2007. Functionality of the β/six site-specific recombination system in. tobacco and Arabidopsis: a novel tool for genetic engineering of plant genomes. Plant Mol Biol. 63:545-556.
13. Gueldener, U., J., J. Heinisch, G. J. Köhler, D. Voss, and J. H. Hegemann. 2002. A second set af loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res. 30:e23.
14. Guidener, U., S. Heck, T. Fielder, J. Beinhauer, and J. H. Hegemann. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res. 24:2519-2524.
15. Hamilton, D. L., and K. Abremski. .1984. Site-specific recombination by the bacteriophage P1. lox-Cxe system. Cre-mediated synapsis of two lox sites. J. MoI. Biol. 178:481-486.
16. Hearn, V. M., and D. W. Mackenzie. 1980. Mycelial antigens from, two strains of Aspergillus fumigatus: an analysis by two-dimensional Immunoelectrophoresis. Mykosen 23:549-562.
17. Hegemann, J. H., U. Güldener, and G. J. Köhler. 2006. Gene disruption in the budding yeast Saccharomyces cerevisiae. Methods Mol. Biol. 313:129144.
18. Kämper, J. 2004. A PCR-based system, for highly efficient generation of gene replacement mutants in Ustilago mayáis. Mol. Genet. Genomics 271:103110.
19. Kilby, N. J., M. R. Snaith, and J. A. Murray. 1993. Site-specific recombinases: tools for genome engineering. Trends Genet. 9:413-421.
20. Krappmann, S. 2006. Tools to study molecular mechanisms oí Aspergillus pathogenicity. Trends Microbiol. 14:356-364.
21. Krappmann, S., Ö. Bayram, and G. H. Braus. 2005. Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module. Eukaryot. Cell 4:1298-1307.
22. Krappmann, S., and G. H. Braus. 2003. Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue. Mol. Genet. Genomics 268:675-683.
23. Krappmann, S., R. Pries, G. Gellissen, M. Hiller, and G. H. Braus. 2000. HARO7 encodes chorismate mutase of the methylotrophic yeast Hansenula polymorpha and is derepressed upon, methanol utilization. J. Bacteriol. 182: 4188-4197.
24. Krappmann, S., C. Sasse, and G. H. Braus. 2006. Gene targeting in Aspergillus fumigatus by homologous recombination, is facilitated in a nonhomologous end-joining-deficient genetic background. Eukaryot. Cell 5:2.12-215.
25. Kubodera, T., N. Yamashita, and A. Nishimura. 2000. Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. Biosci. Biotechnol. Biochem. 64:1416-1421.
26. Kubodera, T., N. Yamashita, and A. Nishimura. 2002. Transformation of Aspergillus sp. and. Trichoderma reesei using the pyrithiamine resistance gene (ptrA) of Aspergillus oryzae. Biosci. Biotechnol. Biochem. 66:404-406. (Pubitemid 39251064)
27. Langfelder, K., B. Jahn, H. Gehringer, A. Schmidt, G. Wanner, and A. A. Brakhage. 1998. Identification of a polyketide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence. Med. Microbiol. Immunol. 187:79-89.
28. McCluskey, K., A. Wiest, and M. Plamann. 2010. The Fungal Genetics Stock Center: a repository for 50 years of fungal genetics research. J. Biosci. 35:119-126.
29. Morschhäuser, J., S. Michel, and P. Staib. 1999. Sequential gene disruption in Candida albicans by FLP-mediated site-specific recombination. Mol. Microbiol. 32:547-556.
30. Morschhäuser, J., P. Staib, and G. Köhler. 2005. Targeted gene deletion in Candida albicans wild-type strains by MPAR flipping. Methods Mbl. Med. 118:35-44.
31. Punt, P. J., and C. A. van den Hondel. 1992. Transformation of filamentous fungi, based on hygromycin B and phleomycin resistance markers. Methods Enzymol. 216:447-457.
32. Reuss, O., A. Vik, R. Kolter, and J. Morschhäuser. 2004. The SAT1 flipper, an optimized tool, for gene disruption in Candida albicans. Gene 341:119127.
33. Rice, P. A. 2005. Resolving integral questions in site-specific recombination. Nat. Struct. Mol. Biol. 12:641-643.
34. Ritt, C., R. Grimm, S. Fernandez, J. C. Alonso, and K. D. Crasser. 1998. Four differently chromatin-associated maize HMG domain proteins modulate DNA structure and act as architectural elements in nucleoprotein complexes. Plant J. 14:623-631.
35. Rojo, F., and J. C. Alonso. 1994. A novel site-specific recombinase encoded by the Streptococcus pyogenes plasmid pSMl.9035. J. Mol. Biol. 238:1.59-172.
36. Rojo, F., and J. C. Alonso. 1995. The ß recombinase of plasmid pSM.19035 binds to two adjacent sites, making different contacts at each of them. Nucleic Acids Res. 23:3181-3188.
37. Rojo, F., F. Weise, and J. C. Alonso. 1993. Purification of the ß product encoded by the Streptococcus pyogenes plasmid pS.M19035. A putative DNA recombinase required to resolve plasmid oligomers. FEBS Lett. 328:169173.
38. Sadowski, P. 1986. Site-specific recombinases: changing partners and doing the twist. J. Bacteriol. 165:341-347.
39. Southern, E. 2006. Southern blotting. Nat. Protoc. 1:51.8-525.
40. Staib, E., M. Kretschmar, T. Nichterlein, H. Hof, and J. Morschhäuser. 2000. Differential activation of a Candida albicans virulence gene family during infection. Proc. Natl. Acad. Sci. U. S. A. 97:6102-61.07.
41. Stemmer, C., S. Fernandez, G. Lopez, J. C. Alonso, and K. D. Grasser. 2002. Plant chromosomal HMGB proteins efficiently promote the bacterial sitespecific ß-mediated recombination in vitro and in vivo. Biochemistry 41: 7763-7770.
42. Szewczyk, E., T. Nayak, C. E. Oakley, H. Edgerton, Y. Xlong, N. TaheriTalesh, S. A. Osmani, and B. R. Oakley. 2006. Fusion PCR and gene targeting in Aspergillus nidulans. Nat. Protoč. 1:3111-3120.
43. Tsai, H. F., Y. C. Chang, R. G. Washburn, M. H. Wheeler, and K. J. Kwon-Chung. 1.998. The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence. J. Bacteriol. 180:3031-3038.
44. Tsai, H. F., M. H. Wheeler, Y. C. Chang, and K. J. Kwon-Chung. 1999. A developmentaly regulated gene cluster involved in. conidial pigment biosynthesis in Aspergillus fumigatus. J. Bacteriol. 181:6469-6477.
45. Wieczorke, R., S. Krampe, T. Weierstall, K. Freidel, C. P. Hollenberg, and E. Boles. 1999. Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett. 464:123-128.
46. Zadra, I., B. Abt, W. Parson, and H. Haas. 2000. xylP promoter-based expression system and its use for antisense downregulation of the Penicillium chrysogenum nitrogen regulator NRE. Appl. Environ. Microbiol. 66:48104816.