Consecutive gene deletions in Aspergillus nidulans: Application of the Cre/loxP system

Current Genetics

Volumn 50, Issue 3, 2006, Pages 217-224

  Forment, Josep V ^a,b   Ramón, Daniel ^a,b   MacCabe, Andrew P ^a  

^a INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Aspergillus nidulans; Filamentous fungi; Functional genomics; Null mutants; Recombinase; Reusable deletion cassette

Indexed keywords

CRE RECOMBINASE; FUNGAL DNA; GLUCOSE; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLAN ENDO 1,3 BETA XYLOSIDASE A; XYLOSE;

ARTICLE; ASPERGILLUS NIDULANS; BACTERIOPHAGE P1; CATALYSIS; CONIDIUM; CONTROLLED STUDY; DNA FLANKING REGION; ENZYME ACTIVITY; FUNCTIONAL GENOMICS; GENE CONSTRUCT; GENE DELETION; GENE EXPRESSION; GENE MUTATION; GENETIC COMPLEMENTATION; GENETIC MARKER; GENETIC RECOMBINATION; NEUROSPORA CRASSA; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION;

ASPERGILLUS NIDULANS; BASE SEQUENCE; DNA, FUNGAL; GENE DELETION; GENES, FUNGAL; INTEGRASES; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINATION, GENETIC;

EMERICELLA NIDULANS; FUNGI; NEUROSPORA CRASSA;

EID: 33747185339     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-006-0081-2     Document Type: Article

References (39)

1. Alani E, Cao L, Kleckner N (1987) A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116:541-545
2. Ballance DJ, Buxton FP, Turner G (1983) Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassa. Biochem Biophys Res Commun 112:284-289
3. Ballance DJ, Turner G (1985) Development of a high-frequency transforming vector for Aspergillus nidulans. Gene 36:321-331
4. Clutterbuck AJ (1972) Absence of laccase from yellow-spored mutants of Aspergillus nidulans. J Gen Microbiol 70:423-435
5. Cove DJ (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim Biophys Acta 113:51-56
6. d'Enfert C (1996) Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5′-decarboxylase gene, pyrG, as a unique transformation marker. Curr Genet 30:76-82
7. Fernández-Cañón JM, Peñalva MA (1995) Overexpression of two penicillin structural genes in Aspergillus nidulans. Mol Gen Genet 246:110-118
8. Fincham JRS (1989) Transformation in fungi. Microbiol Rev 53:148-170
9. Forment JV, Flipphi M, Ramón D, Ventura L, MacCabe AP (2006) Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans. J Biol Chem 281:8339-8346
10. Galagan JE, Calvo SE, Cuomo C, Ma LJ, Wortman JR, Batzoglou S, Lee SI, Basturkmen M, Spevak CC, Clutterbuck J, Kapitonov V, Jurka J, Scazzocchio C, Farman M, Butler J, Purcell S, Harris S, Braus GH, Draht O, Busch S, d'Enfert C, Bouchier C, Goldman GH, Bell-Pedersen D, Griffiths-Jones S, Doonan JH, Yu J, Vienken K, Pain A, Freitag M, Selker EU, Archer DB, Penalva MA, Oakley BR, Momany M, Tanaka T, Kumagai T, Asai K, Machida M, Nierman WC, Denning DW, Caddick M, Hynes M, Paoletti M, Fischer R, Miller B, Dyer P, Sachs MS, Osmani SA, Birren BW (2005) Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438:1105-1115
11. Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH (2002) A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res 30:e23
12. Güldener U, Heck S, Fielder T, Beinhauer J, Hegemann JH (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24:2519-2524
13. Hartl L, Seiboth B (2005) Sequential gene deletions in Hypocrea jecorina using a single blaster cassette. Curr Genet 48:204-211
14. Kozak M (1999) Initiation of translation in prokaryotes and eukaryotes. Gene 234:187-208
15. Krappmann S, Braus GH (2003) Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue. Mol Genet Genomics 268:675-683
16. Krappmann S, Bayram O, Braus GH (2005) Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module. Eukaryot Cell 4:1298-1307
17. Lamy B, Davies J (1991) Isolation and nucleotide sequence of the Aspergillus restrictus gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: The leader sequence protects producing strains from suicide. Nucleic Acids Res 19:1001-1006
18. MacCabe AP, Orejas M, Pérez-González JA, Ramón D (1998) Opposite patterns of expression of two Aspergillus nidulans xylanase genes with respect to ambient pH. J Bacteriol 180:1331-1333
19. Martinelli SD, Kinghorn JR (eds) (1994) Aspergillus: 50 years on. Elsevier, Amsterdam
20. Mayorga ME, Timberlake WE (1992) The developmentally regulated Aspergillus nidulans wA gene encodes a polypeptide homologous to polyketide and fatty acid synthases. Mol Gen Genet 235:205-212
21. Nayak T, Szewczyk E, Oakley CE, Osmani A, Ukil L, Murray SL, Hynes MJ, Osmani SA, Oakley BR (2005) A versatile and efficient gene targeting system for Aspergillus nidulans. Genetics 172:1557-1566
22. Ninomiya Y, Suzuki K, Ishii C, Inoue H (2004) Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining. Proc Natl Acad Sci USA 101:12248-12258
23. Odell J, Caimi P, Sauer B, Russell S (1990) Site-directed recombination in the genome of transgenic tobacco. Mol Gen Genet 223:369-378
24. O'Hara EB, Timberlake WE (1989) Molecular characterisation of the Aspergillus nidulans yA locus. Genetics 121:249-254
25. Orejas M, MacCabe AP, Pérez González JA, Kumar S, Ramón D (1999) Carbon catabolite repression of the Aspergillus nidulans xlnA gene. Mol Microbiol 31:177-184
26. Pérez-González JA, De Graaff LH, Visser J, Ramón D (1996) Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes. Appl Environ Microbiol 62:2179-2182
27. Pérez-González JA, van Peij NN, Bezoen A, MacCabe AP, Ramón D, De Graaff LH (1998) Molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding a beta-xylosidase. Appl Environ Microbiol 64:1412-1419
28. Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Bufton WJ (1953) The genetics of Aspergillus nidulans. Adv Genet 5:141-238
29. Rambosek JA, Kinsey JA (1984) An unstable mutant gene of the am locus of Neurospora results from a small duplication. Gene 27:101-107
30. Sambrook J, Russell DW (2001) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, NY
31. Sauer B (1987) Functional expression of the cre - lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol Cell Biol 7:2087-2096
32. Sauer B, Henderson N (1989) Cre-stimulated recombination at loxP -containing DNA sequences placed into the mammalian genome. Nucleic Acids Res 17:147-161
33. Smith AJ, De Sousa MA, Kwabi-Addo B, Heppell-Parton A, Impey H, Rabbitts P (1995) A site-directed chromosomal translocation induced in embryonic stem cells by Cre- loxP recombination. Nat Genet 9:376-385
34. Specht CA, DiRusso CC, Novotny CP, Ullrich RC (1982) A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal Biochem 119:158-163
35. Storms R, Zheng Y, Li H, Sillaots S, Martínez-Pérez A, Tsang A (2005) Plasmid vectors for protein production, gene expression and molecular manipulations in Aspergillus niger. Plasmid 53:191-204
36. Tilburn J, Scazzocchio C, Taylor GG, Zabicky-Zissman JH, Lockington RA, Davies RW (1983) Transformation by integration in Aspergillus nidulans. Gene 26:205-221
37. Turner G (1994) Vectors for genetic manipulation. In: Martinelli SD, Kinghorn JR (eds) Aspergillus: 50 years on. Elsevier, Amsterdam, pp 641-665
38. Vetter D, Andrews BJ, Roberts-Beatty L, Sadowski PD (1983) Site-specific recombination of yeast 2-micron DNA in vitro. Proc Natl Acad Sci USA 80:7284-7288
39. Wieczorke R, Krampe S, Weierstall T, Freidel K, Hollenberg CP, Boles E (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464:123-128