Characterization of a unique IgG1 mAb CEX profile by limited Lys-C proteolysis/CEX separation coupled with mass spectrometry and structural analysis

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 878, Issue 22, 2010, Pages 1973-1981

  Kim, Jaewon ^a   Jones, Laurie ^a   Taylor, Lisa ^a   Kannan, Gunasekaran ^b   Jackson, Frank ^c   Lau, Hollis ^a   Latypov, Ramil F ^a   Bailey, Bob ^a  

^a Department of Analytical and Formulation Sciences ^*  (United States)

^b Protein Sciences   (United States)

^c AMGEN INC   (United States)

Author keywords

Cation exchange chromatography; Limited Lys C proteolysis; Mass spectrometry; Monoclonal antibody; Structural analysis

Indexed keywords

CATION EXCHANGE CHROMATOGRAPHY; COMBINATORIAL APPROACH; DEAMIDATED; DEAMIDATION; FORCED DEGRADATION; HEAVY CHAIN; HIGH PH; MASS ANALYSIS; METHOD DEVELOPMENT; PEAK AREA; PEPTIDE MAPPING; SEQUENCE ANALYSIS; STABILITY STUDY;

CHEMICAL REACTIONS; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PEPTIDES; POSITIVE IONS; SEPARATION; STABILITY; STRUCTURAL ANALYSIS;

CHROMATOGRAPHY;

ASPARAGINE; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G1; LYSOZYME CHLORIDE; MONOCLONAL ANTIBODY; IMMUNOGLOBULIN G; PEPTIDE;

ACIDITY; ARTICLE; CHEMICAL MODIFICATION; DEAMINATION; DRUG DEGRADATION; DRUG STABILITY; HEAVY CHAIN; ION EXCHANGE CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR MODEL; PEPTIDE MAPPING; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; SEPARATION TECHNIQUE; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; CHO CELL; CRICETULUS; GENETICS; HAMSTER; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; HYDROLYSIS; IMMUNOGLOBULIN G; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PEPTIDES; PROTEIN CONFORMATION;

EID: 77956667212     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2010.05.032     Document Type: Article

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