Cellularity and adipogenic profile of the abdominal subcutaneous adipose tissue from obese adolescents: Association with insulin resistance and hepatic steatosis

Diabetes

Volumn 59, Issue 9, 2010, Pages 2288-2296

  Kursawe, Romy ^a   Eszlinger, Markus ^b   Narayan, Deepak ^a   Liu, Teresa ^c   Bazuine, Merlijn ^a   Cali, Anna M G ^a   D'Adamo, Ebe ^a   Shaw, Melissa ^a   Pierpont, Bridget ^a   Shulman, Gerald I ^a   Cushman, Samuel W ^c   Sherman, Arthur ^c   Caprio, Sonia ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF LEIPZIG   (Germany)

^c NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL COENZYME A CARBOXYLASE; FATTY ACID SYNTHASE; STEROL REGULATORY ELEMENT BINDING PROTEIN 1; INSULIN; LIPID;

ABDOMINAL FAT; ADIPOCYTE; ADIPOGENESIS; ADOLESCENT; ARTICLE; CELL SIZE; CLINICAL ARTICLE; DUAL ENERGY X RAY ABSORPTIOMETRY; FATTY LIVER; FEMALE; GENE EXPRESSION; HUMAN; INSULIN RESISTANCE; LIPOGENESIS; MALE; MICROARRAY ANALYSIS; NUCLEAR MAGNETIC RESONANCE IMAGING; OBESITY; ORAL GLUCOSE TOLERANCE TEST; PHENOTYPE; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SUBCUTANEOUS FAT; ABDOMINAL SUBCUTANEOUS FAT; ANATOMY AND HISTOLOGY; ANCESTRY GROUP; BIOPSY; BODY SIZE; DIASTOLE; GLUCOSE TOLERANCE TEST; INTRAABDOMINAL FAT; PATHOLOGY; PATHOPHYSIOLOGY; PHYSIOLOGY; SIGNAL TRANSDUCTION; SKELETAL MUSCLE; SYSTOLE; WAIST HIP RATIO;

ABDOMINAL FAT; ADOLESCENT; BIOPSY; BODY SIZE; CONTINENTAL POPULATION GROUPS; DIASTOLE; FATTY LIVER; FEMALE; GLUCOSE TOLERANCE TEST; HUMANS; INSULIN; INSULIN RESISTANCE; INTRA-ABDOMINAL FAT; LIPIDS; MALE; MUSCLE, SKELETAL; OBESITY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SUBCUTANEOUS FAT, ABDOMINAL; SYSTOLE; WAIST-HIP RATIO;

EID: 77956383646     PISSN: 00121797     EISSN: 1939327X     Source Type: Journal    
DOI: 10.2337/db10-0113     Document Type: Article

References (45)

1. Danforth E, Jr. Failure of adipocyte differentiation causes type II diabetes mellitus? Nat Genet 2000;26:13
2. Shulman GI. Cellular mechanisms of insulin resistance. J Clin Invest 2000;106:171-176
3. Ravussin E, Smith SR. Increased fat intake, impaired fat oxidation, and failure of fat cell proliferation result in ectopic fat storage, insulin resistance, and type 2 diabetes mellitus. Ann N Y Acad Sci 2002;967:363-378 (Pubitemid 34721922)
4. Sethi JK, Vidal-Puig AJ. Wnt signalling at the crossroads of nutritional regulation. Biochem J 2008;416:e11-e13
5. Azuma K, Heilbronn LK, Albu JB, Smith SR, Ravussin E, Kelley DE, and the Look AHEAD Adipose Research Group. Adipose tissue distribution in relation to insulin resistance in type 2 diabetes mellitus. Am J Physiol Endocrinol Metab 2007;293:E435-E442
6. McLaughlin T, Sherman A, Tsao P, Gonzalez O, Yee G, Lamendola C, Reaven GM, Cushman SW. Enhanced proportion of small adipose cells in insulin-resistant vs insulin-sensitive obese individuals implicates impaired adipogenesis. Diabetologia 2007;50:1707-1715 (Pubitemid 47036653)
7. Spalding KL, Arner E, Westermark PO, Bernard S, Buchholz BA, Bergmann O, Blomqvist L, Hoffstedt J, Näslund E, Britton T, Concha H, Hassan M, Rydén M, Frisén J, Arner P. Dynamics of fat cell turnover in humans. Nature 2008;453:783-787
8. Taksali SE, Caprio S, Dziura J, Dufour S, Calí AM, Goodman TR, Papademetris X, Burgert TS, Pierpont BM, Savoye M, Shaw M, Seyal AA, Weiss R. High visceral and low abdominal subcutaneous fat stores in the obese adolescent: a determinant of an adverse metabolic phenotype. Diabetes 2008;57:367-371
9. Cali' AM, Bonadonna RC, Trombetta M, Weiss R, Caprio S. Metabolic abnormalities underlying the different prediabetic phenotypes in obese adolescents. J Clin Endocrinol Metab 2008;93:1767-1773
10. Matsuda M, DeFronzo RA. Insulin sensitivity indices obtained from oral glucose tolerance testing: comparison with the euglycemic insulin clamp. Diabetes Care 1999;22:1462-1470
11. DeFronzo RA, Tobin JD, Andres R. Glucose clamp technique: a method for quantifying insulin secretion and resistance. Am J Physiol 1979;237:E214-E223
12. Burgert TS, Taksali SE, Dziura J, Goodman TR, Yeckel CW, Papademetris X, Constable RT, Weiss R, Tamborlane WV, Savoye M, Seyal AA, Caprio S. Alanine aminotransferase levels and fatty liver in childhood obesity: associations with insulin resistance, adiponectin, and visceral fat. J Clin Endocrinol Metab 2006;91:4287-4294
13. Cali AM, De Oliveira AM, Kim H, Chen S, Reyes-Mugica M, Escalera S, Dziura J, Taksali SE, Kursawe R, Shaw M, Savoye M, Pierpont B, Constable RT, Caprio S. Glucose dysregulation and hepatic steatosis in obese adolescents: is there a link? Hepatology 2009;49:1896-1903
14. Kim H, Taksali SE, Dufour S, Befroy D, Goodman TR, Petersen KF, Shulman GI, Caprio S, Constable RT. Comparative MR study of hepatic fat quantification using single-voxel proton spectroscopy, two-point dixon and three-point IDEAL. Magn Reson Med 2008;59:521-527
15. Liska D, Dufour S, Zern TL, Taksali S, Cali AM, Dziura J, Shulman GI, Pierpont BM, Caprio, S. Interethnic differences in muscle, liver and abdominal fat partitioning in obese adolescents. PLoS One 2007;2:e569
16. Jo J, Gavrilova O, Pack S, Jou W, Mullen S, Sumner AE, Cushman SW, Periwal V. Hypertrophy and/or Hyperplasia: Dynamics of Adipose Tissue Growth. PLoS Comput Biol 2009;5:e1000324
17. Bower JF, Vadlamudi S, Barakat HA. Ethnic differences in in vitro glyceride synthesis in subcutaneous and omental adipose tissue. Am J Physiol Endocrinol Metab 2002;283:E988-E993
18. Buthelezi EP, van der Merwe MT, Lönnroth PN, Gray IP, Crowther NJ. Ethnic differences in the responsiveness of adipocyte lipolytic activity to insulin. Obes Res 2000;8:171-178
19. Tittelbach TJ, Berman DM, Nicklas BJ, Ryan AS, Goldberg AP. Racial differences in adipocyte size and relationship to the metabolic syndrome in obese women. Obes Res 2004;12:990-998
20. Weiss R, Dufour S, Taksali SE, Tamborlane WV, Petersen KF, Bonadonna RC, Boselli L, Barbetta G, Allen K, Rife F, Savoye M, Dziura J, Sherwin R, Shulman GI, Caprio S. Prediabetes in obese youth: a syndrome of impaired glucose tolerance, severe insulin resistance, and altered myocellular and abdominal fat partitioning. Lancet 2003;362:951-957
21. Tran TT, Yamamoto Y, Gesta S, Kahn CR. Beneficial effects of subcutaneous fat transplantation on metabolism. Cell Metab 2008;7:410-420
22. Gavrilova O, Marcus-Samuels B, Graham D, Kim JK, Shulman GI, Castle AL, Vinson C, Eckhaus M, Reitman ML. Surgical implantation of adipose tissue reverses diabetes in lipoatrophic mice. J Clin Invest 2000;105:271-278
23. MacKellar J, Cushman SW, Periwal, V. Waves of adipose tissue growth in the genetically obese Zucker fatty rat. PLoS One 2010;5:e8197
24. Kim JB, Spiegelman BM. ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Genes Dev 1996;10:1096-1107
25. Kirchgessner TG, Svenson KL, Lusis AJ, Schotz MC. The sequence of cDNA encoding lipoprotein lipase. A member of a lipase gene family. J Biol Chem 1987;262:8463-8466
26. Ong JM, Kern PA. Effect of feeding and obesity on lipoprotein lipase activity, immunoreactive protein, and messenger RNA levels in human adipose tissue. J Clin Invest 1989;84:305-311
27. Farnier C, Krief S, Blache M, Diot-Dupuy F, Mory G, Ferre P, Bazin R. Adipocyte functions are modulated by cell size change: potential involvement of an integrin/ERK signalling pathway. Int J Obes Relat Metab Disord 2003;27:1178-1186
28. Garaulet M, Hernandez-Morante JJ, Lujan J, Tebar FJ, Zamora, S. Relationship between fat cell size and number and fatty acid composition in adipose tissue from different fat depots in overweight/obese humans. Int J Obes (Lond) 2006;30:899-905
29. Jamdar SC, Osborne LJ. Glycerolipid biosynthesis in rat adipose tissue: VIII. Effect of obesity and cell size on [14C]acetate incorporation into lipids. Lipids 1981;16:830-834
30. Ranganathan G, Unal R, Pokrovskaya I, Yao-Borengasser A, Phanavanh B, Lecka-Czernik B, Rasouli N, Kern PA. The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: effects of obesity, insulin resistance, and TZD treatment. J Lipid Res 2006;47:2444-2450
31. Hoffstedt J, Förster D, Löfgren P. Impaired subcutaneous adipocyte lipogenesis is associated with systemic insulin resistance and increased apolipoprotein B/AI ratio in men and women. J Intern Med 2007;262:131-139
32. Roberts R, Hodson L, Dennis AL, Neville MJ, Humphreys SM, Harnden KE, Micklem KJ, Frayn KN. Markers of de novo lipogenesis in adipose tissue: associations with small adipocytes and insulin sensitivity in humans. Diabetologia 2009;52:882-890
33. Savage DB, Petersen KF, Shulman GI. Mechanisms of insulin resistance in humans and possible links with inflammation. Hypertension 2005;45:828-833
34. Yao-Borengasser A, Rasouli N, Varma V, Miles LM, Phanavanh B, Starks TN, Phan J, Spencer HJ 3rd, McGehee RE Jr, Reue K, Kern PA. Lipin expression is attenuated in adipose tissue of insulin-resistant human subjects and increases with peroxisome proliferator-activated receptor gamma activation. Diabetes 2006;55:2811-2818
35. Phan J, Reue K. Lipin, a lipodystrophy and obesity gene. Cell Metab 2005;1:73-83
36. Phan J, Péterfy M, Reue K. Lipin expression preceding peroxisome proliferator-activated receptor-gamma is critical for adipogenesis in vivo and in vitro. J Biol Chem 2004;279:29558-29564
37. Péterfy M, Phan J, Xu P, Reue K. Lipodystrophy in the fld mouse results from mutation of a new gene encoding a nuclear protein, lipin. Nat Genet 2001;27:121-124
38. Yang X, Jansson PA, Nagaev I, Jack MM, Carvalho E, Sunnerhagen KS, Cam MC, Cushman SW, Smith U. Evidence of impaired adipogenesis in insulin resistance. Biochem Biophys Res Commun 2004;317:1045-1051
39. Bogacka I, Xie H, Bray GA, Smith SR. The effect of pioglitazone on peroxisome proliferator-activated receptor-gamma target genes related to lipid storage in vivo. Diabetes Care 2004;27:1660-1667
40. Weyer C, Foley JE, Bogardus C, Tataranni PA, Pratley RE. Enlarged subcutaneous abdominal adipocyte size, but not obesity itself, predicts type II diabetes independent of insulin resistance. Diabetologia 2000;43:1498-1506
41. Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U, Speed TP. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 2003;4:249-264
42. Mootha VK, Lindgren CM, Eriksson KF, Subramanian A, Sihag S, Lehar J, Puigserver P, Carlsson E, Ridderstråle M, Laurila E, Houstis N, Daly MJ, Patterson N, Mesirov JP, Golub TR, Tamayo P, Spiegelman B, Lander ES, Hirschhorn JN, Altshuler D, Groop LC. PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Nat Genet 2003;34:267-273
43. Eszlinger M, Krohn K, Berger K, Läuter J, Kropf S, Beck M, Führer D, Paschke R. Gene expression analysis reveals evidence for increased expression of cell cycle-associated genes and Gq-protein-protein kinase C signaling in cold thyroid nodules. J Clin Endocrinol Metab 2005;90:1163-1170
44. Westfall PH, Young SS. Resampling-based Multiple Testing: Examples and Methods for P-value Adjustment. New York, John Wiley & Sons, 1993
45. van Iersel MP, Kelder T, Pico AR, Hanspers K, Coort S, Conklin BR, Evelo C. Presenting and exploring biological pathways with PathVisio. BMC Bioinformatics 2008;9:399