Validation of endogenous reference genes for RT-qPCR normalisation in bovine lymphoid cells (BL-3) infected with Bovine Viral Diarrhoea Virus (BVDV)

Veterinary Immunology and Immunopathology

Volumn 137, Issue 3-4, 2010, Pages 201-207

  Anstaett, Olivia L ^a   Brownlie, Joe ^a   Collins, Margaret E ^a   Thomas, Carole J ^a  

^a ROYAL VETERINARY COLLEGE   (United Kingdom)

Author keywords

BVDV; Normalisation; Real time PCR; Reference genes; RT qPCR

Indexed keywords

ALPHA 1 MICROGLOBULIN; BETA ACTIN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; TRYPTOPHAN HYDROXYLASE; TYROSINE 3 MONOOXYGENASE;

ANIMAL CELL; ARTICLE; BOVINE VIRAL DIARRHEA; CELL CULTURE; CONTROLLED STUDY; COW; GENE; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC STABILITY; GENETIC VARIABILITY; LYMPHOID CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE STUDY; REFERENCE GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS; VIRUS STRAIN;

ANIMALS; CATTLE; CELLS, CULTURED; DIARRHEA VIRUSES, BOVINE VIRAL; GENE EXPRESSION; LYMPHOCYTES; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

BOVINAE; BOVINE VIRAL DIARRHEA VIRUS 1;

EID: 77956268942     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2010.05.006     Document Type: Article

References (28)

1. Andersen C.L., Jensen J.L., Orntoft T.F. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004, 64:5245-5250.
2. Bionaz M., Loor J.J. Identification of reference genes for quantitative real-time PCR in the bovine mammary gland during the lactation cycle. Physiol. Genomics 2007, 29:312-319.
3. Brinkhof B., Spee B., Rothuizen J., Penning L.C. Development and evaluation of canine reference genes for accurate quantification of gene expression. Anal. Biochem. 2006, 356:36-43.
4. Bustin S.A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J. Mol. Endocrinol. 2002, 29:23-39.
5. Bustin S.A., Benes V., Garson J.A., Hellemans J., Huggett J., Kubista M., Mueller R., Nolan T., Pfaffl M.W., Shipley G.L., Vandesompele J., Wittwer C.T. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 2009, 55:611-622.
6. Bustin S.A., Nolan T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Technol. 2004, 15:155-166.
7. Dheda K., Huggett J.F., Chang J.S., Kim L.U., Bustin S.A., Johnson M.A., Rook G.A., Zumla A. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal. Biochem. 2005, 344:141-143.
8. Glare E.M., Divjak M., Bailey M.J., Walters E.H. beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels. Thorax 2002, 57:765-770.
9. Gutierrez L., Mauriat M., Guenin S., Pelloux J., Lefebvre J.F., Louvet R., Rusterucci C., Moritz T., Guerineau F., Bellini C., Van Wuytswinkel O. The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. Plant Biotechnol. J. 2008, 6:609-618.
10. Houe H. Epidemiological features and economical importance of bovine virus diarrhoea virus (BVDV) infections. Vet. Microbiol. 1999, 64:89-107.
11. Huggett J., Dheda K., Bustin S., Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 2005, 6:279-284.
12. Li Y.P., Bang D.D., Handberg K.J., Jorgensen P.H., Zhang M.F. Evaluation of the suitability of six host genes as internal control in real-time RT-PCR assays in chicken embryo cell cultures infected with infectious bursal disease virus. Vet. Microbiol. 2005, 110:155-165.
13. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408.
14. Nygaard A.B., Jorgensen C.B., Cirera S., Fredholm M. Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR. BMC Mol. Biol. 2007, 8:67.
15. Perez R., Tupac-Yupanqui I., Dunner S. Evaluation of suitable reference genes for gene expression studies in bovine muscular tissue. BMC Mol. Biol. 2008, 9:79.
16. Pfaffl M.W., Tichopad A., Prgomet C., Neuvians T.P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 2004, 26:509-515.
17. Radonic A., Thulke S., Mackay I.M., Landt O., Siegert W., Nitsche A. Guideline to reference gene selection for quantitative real-time PCR. Biochem. Biophys. Res. Commun. 2004, 313:856-862.
18. Ridpath J.F., Bendfeldt S., Neill J.D., Liebler-Tenorio E. Lymphocytopathogenic activity in vitro correlates with high virulence in vivo for BVDV type 2 strains: criteria for a third biotype of BVDV. Virus Res. 2006, 118:62-69.
19. Robinson T.L., Sutherland I.A., Sutherland J. Validation of candidate bovine reference genes for use with real-time PCR. Vet. Immunol. Immunopathol. 2007, 115:160-165.
20. Roge R., Thorsen J., Torring C., Ozbay A., Moller B.K., Carstens J. Commonly used reference genes are actively regulated in in vitro stimulated lymphocytes. Scand. J. Immunol. 2007, 65:202-209.
21. Schmittgen T.D., Zakrajsek B.A. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J. Biochem. Biophys. Methods 2000, 46:69-81.
22. Selvey S., Thompson E.W., Matthaei K., Lea R.A., Irving M.G., Griffiths L.R. Beta-actin-an unsuitable internal control for RT-PCR. Mol. Cell. Probes 2001, 15:307-311.
23. Skovgaard K., Mortensen S., Poulsen K.T., Angen O., Heegaard P.M. Validation of putative reference genes for qRT-PCR normalization in tissues and blood from pigs infected with Actinobacillus pleuropneumoniae. Vet. Immunol. Immunopathol. 2007, 118:140-146.
24. Spinsanti G., Panti C., Lazzeri E., Marsili L., Casini S., Frati F., Fossi C.M. Selection of reference genes for quantitative RT-PCR studies in striped dolphin (Stenella coeruleoalba) skin biopsies. BMC Mol. Biol. 2006, 7:32.
25. Strube C., Buschbaum S., Wolken S., Schnieder T. Evaluation of reference genes for quantitative real-time PCR to investigate protein disulfide isomerase transcription pattern in the bovine lungworm Dictyocaulus viviparus. Gene 2008, 425:36-43.
26. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3. RESEARCH0034.
27. Wood S.H., Clements D.N., McEwan N.A., Nuttall T., Carter S.D. Reference genes for canine skin when using quantitative real-time PCR. Vet. Immunol. Immunopathol. 2008, 126(3-4):392-395.
28. Young N.J., Thomas C.J., Collins M.E., Brownlie J. Real-time RT-PCR detection of Bovine Viral Diarrhoea virus in whole blood using an external RNA reference. J. Virol. Methods 2006, 138:218-222.