Validation of candidate bovine reference genes for use with real-time PCR

Veterinary Immunology and Immunopathology

Volumn 115, Issue 1-2, 2007, Pages 160-165

  Robinson, T L ^a   Sutherland, I A ^a   Sutherland, J ^a  

^a CSIRO   (Australia)

Author keywords

Actin; 18S rRNA; Acidic ribosomal protein; Bovine; GAPDH; Housekeeping genes; Real time PCR; Reference genes

Indexed keywords

ACIDIC RIBOSOMAL PROTEIN LARGE; BETA ACTIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; PROTEIN; RNA 18S;

18S RNA GENE; ACIDIC RIBOSOMAL PROTEIN LARGE GENE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BETA ACTIN GENE; BOOPHILUS MICROPLUS; CATTLE; COMPARATIVE STUDY; CONTROLLED STUDY; DNA FINGERPRINTING; GENE; GENE EXPRESSION; GENOTYPE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE GENE; HOUSEKEEPING GENE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TICK;

ACTINS; ANIMALS; CATTLE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; PHOSPHOPROTEINS; POLYMERASE CHAIN REACTION; RIBOSOMAL PROTEINS; RNA, RIBOSOMAL, 18S;

BOOPHILUS MICROPLUS; BOS TAURUS;

EID: 33845439391     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2006.09.012     Document Type: Article

References (23)

1. Andersen C.L., Jensen J.L., and Ørntoft T.F. Normalisation of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64 (2004) 5245-5250
2. Applied Biosystems, Sequence Detection Systems ABI Prism® 7000HT, 7000, 7700; GeneAmp® 5700 Chemistry Guide, Applied Biosystems, 2003.
3. Dheda K., Huggett J.F., Bustin S.A., Johnson M.A., Rook G., and Zumla A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. BioTechniques 37 (2004) 112-119
4. Donaldson L., Vuocolo T., Gray C., Strandberg Y., Reverter T., McWilliam S., Wang Y., Byrne K., and Tellam R. Construction and validation of a bovine innate immune microarray. BMC Genomics 6 (2005) 135
5. Frost P., and Nilsen F. Validation of reference genes for transcription profiling in the salmon louse Lepeophtheirus salmonis by quantitative real-time PCR. Vet. Parasitol. 118 (2003) 169-174
6. García-Vallejo J.J., Van het Hof B., Robben J., Van Wijk J.A.E., Van Die I., Joziasse D.H., and Van Dijk K. Approach for defining endogenous reference genes in gene expression experiments. Anal. Biochem. 329 (2004) 293-299
7. Goiden D., Mamessier A., Staquet M., Schmitt D., and Berthier-Vergnes O. Ribosomal 18S rRNA prevails over glyceraldehyde-3-phosphate dehydrogenase and β-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and non-invasive human melanoma cell subpopulations. Anal. Biochem. 295 (2001) 17-21
8. Huggett J., Dheda K., Bustin S., and Zumla A. Real-time PCR normalisation; strategies and considerations. Genes Immunity 6 (2005) 279-284
9. Jemiolo B., and Trappe S. Single muscle fiber gene expression in human skeletal muscle: validation of internal control with exercise. Biochem. Biophys. Res. Commun. 320 (2004) 1043-1050
10. Konnai S., Usui T., Ohashi K., and Onuma M. The rapid quantitative analysis of bovine cytokine genes by real-time RT-PCR. Vet. Microbiol. 94 (2003) 293-294
11. Leutenegger C.M., Alluwaimi A.M., Smith W.L., Perani L., and Cullor J.S. Quantification of bovine cytokine mRNA in milk cells of healthy cattle by real-time Taqman polymerase chain reaction. Vet. Immunol. Immunopath. 77 (2000) 275-287
12. McGuire K., Manuja A., Russell G.C., Sprinbett A., Craigmile S.C., Nichani A.K., Malhotra D.V., and Glass E.J. Quantitative analysis of pro-inflammatory cytokine mRNA expression in Theileria annulata-infected cell lines derived from resistant and susceptible cattle. Vet. Immunol. Immunopath. 99 (2004) 87-98
13. Mena A., Ioannou X.P., Van Kessel A., Van Drunen Little-Van Den Hurk S., Popowych Y., Babiuk L.A., and Godson D.L. Th1/Th2 biasing effects of vaccination in cattle as determined by real-time PCR. J. Immunol. Method 263 (2002) 11-21
14. Muller P.Y., Janovjak H., Miserez A.R., and Dobbie Z. Processing of gene expression data generated by quantitative real-time RT-PCR. BioTechniques 32 (2002) 6
15. Pfaffl M.W., Tichopad A., Prgomet C., and Neuvians T.P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26 (2004) 509-515
16. Radonić A., Thulke S., Mackay I.M., Landt O., Siegert W., and Nitsche A. Guideline to reference gene selection for quantitative real-time PCR. Biochem. Biophys. Res. Commun. 313 (2004) 856-862
17. Schmid H., Cohen C.D., Henger A., Irrgang S., Schlöndorff D., and Kretzler M. Validation of endogenous controls for gene expression analysis in microdissected human renal biopsies. Kidney Int. 64 (2003) 356-360
18. Schmittgen T.D., and Zakrajsek B.A. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J. Biochem. Biophys. Method 46 (2000) 69-81
19. Selvey S., Thompson E.W., Matthaei K., Lea R.A., Irving M.G., and Griffiths L.R. Beta-actin-an unsuitable internal control for RT-PCR. Mol. Cell. Probe 15 (2001) 307-311
20. Thellin O., Zorzi W., Lakaye B., De Borman B., Coumans B., Hennen G., Grisar T., Igout A., and Heinen E. Housekeeping genes as internal standards: use and limits. J. Biotechnol. 75 (1999) 291-295
21. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., and Speleman F. Accurate normalisation of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3 (2002) 7
22. Waldvogel A.S., Hediger-Weithaler B.M., Eicher R., Zakher A., Zarlenga D.S., Gasbarre L.C., and Heussler V.T. Interferon-γ and interleukin-4 mRNA expression by peripheral blood mononuclear cells from pregnant and non-pregnant cattle seropositive for bovine viral diarrhea virus. Vet. Immunol. Immunopath. 77 (2000) 201-212
23. Yakobson B., Brenner J., Ungar-Waron H., and Trainin Z. Short-termed expression of interleukin-2 during experimental BLV infection may direct disease progression to persistent lymphocytosis. Vet. Immunol. Immunopath. 64 (1998) 207-218