Reference genes for canine skin when using quantitative real-time PCR

Veterinary Immunology and Immunopathology

Volumn 126, Issue 3-4, 2008, Pages 392-395

  Wood, Shona H ^a,b   Clements, Dylan N ^c   McEwan, Neil A ^a   Nuttall, Tim ^a   Carter, Stuart D ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Atopic dermatitis; GAPDH; qPCR; Reference genes

Indexed keywords

FENTICLOR; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INOSINE PHOSPHATE; MESSENGER RNA;

ANIMAL TISSUE; ARTICLE; ATOPIC DERMATITIS; CG 14980 GENE; CONTROLLED STUDY; DOG; FEMALE; GENE; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETIC STABILITY; HIRA GENE; IMP GENE; MALE; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RPL13A GENE; S7 GENE; SDHA GENE; SKIN; STATISTICAL ANALYSIS;

ANIMALS; DOGS; GENE EXPRESSION; PROTEINS; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SKIN;

CANIS FAMILIARIS;

EID: 56149088125     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2008.08.006     Document Type: Article

References (16)

1. Andersen C.L., Jensen J.L., and Orntoft T.F. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization. Applied to bladder and colon cancer data sets. Cancer Research 64 (2004) 5245-5250
2. Brinkhof B., Spee B., Rothuizen J., and Penning L.C. Development and evaluation of canine reference genes for accurate quantification of gene expression. Analytical Biochemistry 356 (2006) 36-43
3. Bustin S.A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. Journal of Molecular Endocrinology 29 (2002) 23-39
4. Bustin S.A., and Nolan T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. Journal of Biomolecular Technology 15 (2004) 155-166
5. Clements D.N., Vaughan-Thomas A., Peansukmanee S., Carter S.D., Innes J.F., Ollier W.E., and Clegg P.D. Assessment of the use of RNA quality metrics for the screening of articular cartilage specimens from clinically normal dogs and dogs with osteoarthritis. American Journal of Veterinary Research 67 (2006) 1438-1444
6. Clements D.N., Carter S.D., Innes J., Ollier W., and Day P. Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction. Arthritis Research and Therapy 8 (2006) R158
7. DeBoer D.J., and Hillier A. The ACVD task force on canine atopic dermatitis (XV): fundamental concepts in clinical diagnosis. Veterinary Immunology and Immunopathology 81 (2001) 271-276
8. Dheda K., Huggett J.F., Bustin S.A., Johnson M.A., Rook G., and Zumla A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 37 (2004) 112-119
9. Huggett J., Dheda K., Bustin S., and Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes and Immunity 6 (2005) 279-284
10. Imbeaud S., Graudens E., Boulanger V., Barlet X., Zaborski P., Eveno E., Mueller O., Schroeder A., and Auffray C. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Research 33 (2005) e56
11. Lekanne Deprez R.H., Fijnvandraat A.C., Ruijter J.M., and Moorman A.F.M. Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions. Analytical Biochemistry 307 (2002) 63-69
12. Maccoux L., Clements D.N., Salway F., and Day P. Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data. BMC Molecular Biology 8 (2007) 62
13. Penning L.C., Vrieling H.E., Brinkhof B., Riemers F.M., Rothuizen J., Rutteman G.R., and Hazewinkel HAW. A validation of 10 feline reference genes for gene expression measurements in snap-frozen tissues. Veterinary Immunology and Immunopathology 120 (2007) 212-222
14. Peters I.R., Peeters D., Helps C.R., and Day M.J. Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies. Veterinary Immunology and Immunopathology 117 (2007) 55-66
15. Pfaffl M.W., Tichopad A., Prgomet C., and Neuvians T.P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnology Letters 26 (2004) 509-515
16. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., and Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 3 (2002) (research 0034)