Validation of putative reference genes for qRT-PCR normalization in tissues and blood from pigs infected with Actinobacillus pleuropneumoniae

Veterinary Immunology and Immunopathology

Volumn 118, Issue 1-2, 2007, Pages 140-146

  Skovgaard, Kerstin ^a   Mortensen, Shila ^a   Poulsen, Karin T ^a   Angen, Øystein ^a   Heegaard, Peter M H ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Gene expression; geNorm; Normfinder; Porcine pleuropneumonia; qRT PCR; Reference genes

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INTERLEUKIN 6;

ACTINOBACILLUS PLEUROPNEUMONIAE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BACTERIAL INFECTION; BLOOD; BLOOD CELL; COMPARATIVE STUDY; CONTROLLED STUDY; GENE EXPRESSION; LIVER; LYMPH NODE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SWINE; TONSIL; UPREGULATION; VALIDATION PROCESS;

ACTINOBACILLUS INFECTIONS; ACTINOBACILLUS PLEUROPNEUMONIAE; ANIMALS; GENE EXPRESSION REGULATION; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SPECIFIC PATHOGEN-FREE ORGANISMS; SWINE; SWINE DISEASES;

ACTINOBACILLUS PLEUROPNEUMONIAE; ANIMALIA; MYCOPLASMA; SUIDAE; SUS;

EID: 34250768852     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2007.04.010     Document Type: Article

References (27)

1. Andersen C.L., Jensen J.L., and Ørntoft T.F. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64 (2004) 5245-5250
2. Baarsch M.J., Scamurra R.W., Burger K., Foss D.L., Maheswaran S.K., and Murtaugh M.P. Inflammatory cytokine expression in swine experimentally infected with Actinobacillus pleuropneumoniae. Infect. Immun. 63 (1995) 3587-3594
3. Baarsch M.J., Foss D.L., and Murtaugh M.P. Pathophysiologic correlates of acute porcine pleuropneumonia. Am. J. Vet. Res. 61 (2000) 684-690
4. Bosse J.T., Janson H., Sheehan B.J., Beddek A.J., Rycroft A.N., Kroll J.S., and Langford P.R. Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection. Microbes Infect. 4 (2002) 225-235
5. Bustin S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25 (2000) 169-193
6. Cummings C.A., and Relman D.A. Using DNA microarrays to study host-microbe interactions. Emerg. Infect. Dis. 6 (2000) 513-525
7. Fossum C., Wattrang E., Fuxler L., Jensen K.T., and Wallgren P. Evaluation of various cytokines (IL-6, IFN-alpha, IFN-gamma, TNF-alpha) as markers for acute bacterial infection in swine a possible role for serum interleukin-6. Vet. Immunol. Immunopathol. 64 (1998) 161-172
8. Garcia-Crespo D., Juste R.A., and Hurtado A. Selection of ovine housekeeping genes for normalization by real-time RT-PCR; analysis of PrP gene expression and genetic susceptibility to scrapie. BMC Vet. Res. 1 (2005) 3
9. Giese S., Stenbæk E., and Nielsen R. Identification of Actinobacillus pleuropneumoniae serotype 2 by monoclonal or polyclonal antibodies in latex agglutination tests. Acta Vet. Scand. 34 (1993) 223-225
10. Goossens K., Van Poucke M., Van Soom A., Vandesompele J., Van Zeveren A., and Peelman L.J. Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos. BMC Dev. Biol. 5 (2005) 27
11. Hines M.E., and Frazier K.S. Expression of inflammatory cytokine mRNA in lymphoid tissue from swine experimentally infected with Mycobacterium avium serovar 2. Am. J. Vet. Res. 61 (2000) 1487-1491
12. Huang H., Potter A.A., Campos M., Leighton F.A., Willson P.J., Haines D.M., and Yates W.D. Pathogenesis of porcine Actinobacillus pleuropneumonia, part II: roles of proinflammatory cytokines. Can. J. Vet. Res. 63 (1999) 69-78
13. Huggett J., Dheda K., Bustin S., and Zumla A. Real-time RT-PCR normalization; strategies and considerations. Genes Immun. 6 (2005) 279-284
14. Hultén C., Johansson E., Fossum C., and Wallgren P. Interleukin 6, serum amyloid A and haptoglobin as markers of treatment efficacy in pigs experimentally infected with Actinobacillus pleuropneumoniae. Vet. Microbiol. 95 (2003) 75-89
15. Johansson E., Fossum C., Fuxler L., and Wallgren P. Effects of an experimental infection with Actinobacillus pleuropneumoniae on the interferon-alpha and interleukin-6 producing capacity of porcine peripheral blood mononuclear cells stimulated with bacteria, virus or plasmid DNA. Vet. Microbiol. 79 (2001) 171-182
16. Kristensen C.S., Angen O., Andreasen M., Takai H., Nielsen J.P., and Jorsal S.E. Demonstration of airborne transmission of Actinobacillus pleuropneumoniae serotype 2 between simulated pig units located at close range. Vet. Microbiol. 98 (2004) 243-249
17. Li H. Gene sequencing. China, Denmark team up to tackle the pig. Science 290 (2000) 913-914
18. Moser R.J., Reverter A., Kerr C.A., Beh K.J., and Lehnert S.A. A mixed-model approach for the analysis of cDNA microarray gene expression data from extreme-performing pigs after infection with Actinobacillus pleuropneumoniae. J. Anim. Sci. 82 (2004) 1261-1271
19. Nicot N., Hausman J.F., Hoffmann L., and Evers D. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J. Exp. Bot. 56 (2005) 2907-2914
20. Nielsen K.K., and Boye M. Real-time quantitative reverse transcription-PCR analysis of expression stability of Actinobacillus pleuropneumoniae housekeeping genes during in vitro growth under iron-depleted conditions. Appl. Environ. Microbiol. 71 (2005) 2949-2954
21. Olsvik P.A., Lie K.K., Jordal A.E., Nilsen T.O., and Hordvik I. Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon. BMC Mol. Biol. 6 (2005) 21
22. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 1 (2001) 29
23. Radonic A., Thulke S., Mackay I.M., Landt O., Siegert W., and Nitsche A. Guideline to reference gene selection for quantitative real-time PCR. Biochem. Biophys. Res. Commun. 313 (2004) 856-862
24. Salguero F.J., Sanchez-Cordon P.J., Nunez A., Fernandez, de Marco M., and Gomez-Villamandos J.C. Proinflammatory cytokines induce lymphocyte apoptosis in acute African swine fever infection. J. Comp. Pathol. 132 (2005) 289-302
25. Seah J.N., and Kwang J. Localization of linear cytotoxic and pro-apoptotic epitopes in RTX toxin ApxIII of Actinobacillus pleuropneumoniae. Vaccine 22 (2004) 1494-1497
26. Skovgaard K., Grell S.N., Heegaard P.M., Jungersen G., Pudrith C.B., and Coussens P.M. Differential expression of genes encoding CD30L and P-selectin in cattle with Johne's disease: progress toward a diagnostic gene expression signature. Vet. Immunol. Immunopathol. 112 (2006) 210-224
27. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., and Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3 (2002) 7