Ochratoxigenic fungi and ochratoxin A in cocoa during farm processing

International Journal of Food Microbiology

Volumn 143, Issue 1-2, 2010, Pages 67-70

  Copetti, Marina V ^a   Pereira, José Luís ^b   Iamanaka, Beatriz T ^c   Pitt, John I ^d   Taniwaki, Marta H ^c  

^a FEDERAL UNIVERSITY OF SANTA MARIA   (Brazil)

^b UNIVERSITY OF CAMPINAS   (Brazil)

^c INSTITUTO DE TECNOLOGIA DE ALIMENTOS   (Brazil)

^d CSIRO   (Australia)

Author keywords

Aspergillus carbonarius; Aspergillus niger aggregate; HPLC; Ochratoxigenic fungi; Ochratoxin A; Theobroma cacao

Indexed keywords

OCHRATOXIN; WATER;

ARTICLE; ASPERGILLUS CARBONARIUS; ASPERGILLUS NIGER; ASPERGILLUS NIGER AGGREGATE; CACAO; FARMING SYSTEM; FUNGAL STRAIN; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; OCHRATOXIGENIC FUNGI; STORAGE;

ASPERGILLUS; BRAZIL; CACAO; FOOD CONTAMINATION; OCHRATOXINS; SEEDS;

ASPERGILLUS CARBONARIUS; ASPERGILLUS NIGER; FUNGI; THEOBROMA CACAO;

EID: 77956181539     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2010.07.031     Document Type: Article

References (40)

1. Abarca M.L., Bragulat M.R., Castella G., Cabanes F.J. Ochratoxin A production by strains of Aspergillus niger var. niger. Applied and Environmental Microbiology 1994, 60:2650-2652.
2. Abarca M.L., Accensi F., Bragulat M.R., Castellá G., Cabanes F.J. Aspergillus carbonarius as the main source of ochratoxin A contamination in dried vine fruits from Spanish market. Journal of Food Protection 2003, 66:504-506.
3. Amezqueta S., Gonzalez-Penas E., Murillo M., Lopez de Cerain A. Validation of a high-performance liquid chromatography analytical method for ochratoxin A quantification in cocoa beans. Food Additives and Contaminants 2004, 22:590-596.
4. Amezqueta S., Gonzalez-Penas E., Dachoupakan C., Murillo-Arbizu M., Lopez de Cerain A., Guiraud J.P. OTA-producing fungi isolated from stored cocoa beans. Letters in Applied Microbiology 2008, 47:197-201.
5. Belli N., Pardo E., Marín S., Farre G., Ramos A.J., Sanchis V. Occurrence of ochratoxin A and toxigenic potential of fungal isolates from Spanish grapes. Journal of the Science of Food and Agriculture 2004, 84:541-546.
6. Beuchat L.R. Influence of water activity on sporulation, germination, outgrowth, and toxin production. Water Activity: Theory and Applications to Food 1987, 153-172. Marcel Dekker, New York. L.D. Rockland, L.R. Beuchat (Eds.).
7. Bonvehi J.S. Occurrence of ochratoxin A in cocoa products and chocolate. Journal of Agricultural and Food Chemistry 2004, 52:6347-6352.
8. Boudra H., Le Bars P., Le Bars J. Thermostability of ochratoxin A in wheat under two moisture conditions. Applied and Environmental Microbiology 1995, 61:1156-1158.
9. Burdaspal P.A., Legarda T.M. Ocratoxina A en distintos tipos de chocolate y preparados de cacao en polvo comercializados en Espana y en otros países extranjeros. Alimentaria 2003, 347:143-153.
10. Commission Regulation, 2010. Commission Regulation (EU) No 105/2010 of 5 February 2010 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards ochratoxin A. Available at: (). Acessed in 26/06/2010. http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:035:0007:0008:EN:PDF.
11. Dongo L., Bandyopadhyay R., Kumar M., Ojiambo P.S. Occurrence of ochratoxin A in Nigerian ready for sale cocoa beans. Agricultural Journal 2008, 3:4-9.
12. Ferraz M.M., Farah A., Iamanaka B., Perrone D., Copetti M.V., Marques V.X., Vitali A., Taniwaki M.H. Kinetics of ochratoxin destruction during coffee roasting. Food Control 2010, 21:872-877.
13. Filtenborg O., Frisvald J.C., Svendsen J.A. Simple screening method for molds producing intracellular mycotoxins in pure cultures. Applied and Environmental Microbiology 1983, 45:581-585.
14. Frisvad J.C., Frank J.M., Houbraken J.A.M.P., Kuijpers A.F.A., Samson R.A. New ochratoxin A producing species of Aspergillus section Circumdati. Studies in Mycology 2004, 50:23-43.
15. Gilmour M., Lindblom M. Management of ochratoxin A in the cocoa supply chain: a summary of work by the CAOBISCO/ECA/FCC working group on ochratoxin A. Mycotoxins: Detection Methods, Management, Public Health and Agricultural Trade 2008, 231-243. CABI, Wallingford, UK. J.F. Leslie, R. Bandyopadhyay, A. Visconti (Eds.).
16. Horie Y. Productivity of ochratoxin A of Aspergillus carbonarius in Aspergillus section Nigri. Nippon Kingakukai Kaiho 1995, 36:73-76.
17. Iamanaka B.T., Taniwaki M.H., Menezes C.H., Vicente E., Fungaro M.H.P. Incidence of toxigenic fungi and ochratoxin A in dried fruits sold in Brazil. Food Additives and Contaminants 2005, 22:1258-1263.
18. Joosten H.M.L.J., Goetz J., Pittet A., Schellenberg M., Bucheli P. Production of ochratoxin A by Aspergillus carbonarius on coffee cherries. International Journal of Food Microbiology 2001, 65:39-44.
19. Klich M.A., Pitt J.I. A Laboratory Guide to Common Aspergillus Species and their Teleomorphs 1988, CSIRO Division of Food Research, North Ryde, N.S.W, 116 pp.
20. Leong S.L., Hocking A.D., Pitt J.I., Kazi B.A., Emmeti R.W., Scott E.S. Australian research on ochratoxin fungi and ochratoxin A. International Journal of Food Microbiology 2006, 111(Suppl. 1):S10-S17.
21. Leong S.-L., Hien L.T., An T.V., Trang N.T., Hocking A.D., Scott E.S. Ochratoxin A-producing Aspergilli in Vietnamese green coffee beans. Letters in Applied Microbiology 2007, 45:301-306.
22. MacDonald S., Wilson P., Barnes K., Damant A., Massey R., Mortby E., Shepherd M.J. Ochratoxin A in dried vine fruit method development and survey. Food Additives and Contaminants 1999, 16:253-260.
23. Magnoli C., Violante M., Combina M., Palacio G., Dalcero A. Mycoflora and ochratoxin-producing strains of Aspergillus section Nigri in wine grapes in Argentina. Letters in Applied Microbiology 2003, 37:179-184.
24. Magnoli C., Astoreca A., Ponsone M.L., Fernández-Juri M.G., Barberis Carla, Dalcero A.M. Ochratoxin A and Aspergillus section Nigri in peanut seeds at different months of storage in Córdoba. Argentina International Journal of Food Microbiology 2007, 119:213-218.
25. Martínez-Culebras P.V., Ramón D. An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine. International Journal of Food Microbiology 2007, 113:147-153.
26. Minifie B.W. Chocolate, Cocoa, and Confectionery: Science and Technology 1999, Van Nostrand & Reinhold, New York, 907 pp. 3rd edn.
27. Miraglia, M., Brera, C. 2002. Reports on tasks for scientific cooperation, task 3.2.7. Available at: (). Acessed in 23/04/2006. http://europa.eu.int/comm/food/fs/scoop/3.2.7_en.pdf.
28. Mounjouenpou P., Gueule D., Fontana-Tachon A., Guyot B., Tondje P.R., Guiraud J.P. Filamentous fungi producing ochratoxin A during cocoa processing in Cameroon. International Journal of Food Microbiology 2008, 128:234-241.
29. Pitt J.I. A Laboratory Guide to Common Penicillium Species 2000, Food Science Australia, North Ryde, N.S.W, 197 pp. 3rd edn.
30. Pitt J.I., Hocking A.D. Fungi and Food Spoilage 2009, Springer Dordrecht, Heidelberg, London, New York, 519 pp. 3rd edn.
31. Pittet A., Tornare D., Huggett A., Viani R. Liquid chromatographic determination of ochratoxin A in pure and adulterated soluble coffee using an immunoaffinity column cleanup procedure. Journal of Agricultural and Food Chemistry 1996, 44:3564-3569.
32. Raters, M., Matissek, R. 2000. Vorkommen der Mykotoxine Aflatoxin B1, B2, G1, G2 und Ochratoxin A in Kakao und Kakaoprodukten. Projekt Nr. 9 der Stiftung der Deutschen Kakao- und Schokoladenwirtschaft, Hamburg. Available at: (). Acessed in 28/03/09. http://www.kakao-stiftung.de/menu4_frameset_e.htmL.
33. Rodgers J.L., Nicewander W.A. Thirteen ways to look at the correlation coefficient. American Statistician 1988, 32:59-66.
34. Samson R.A., Hoekstra E.S., Frisvad J.C., Filtenborg O. Introduction to Food- and Airborne Fungi 2002, Centraalbureau voor Schimmelcultures, Utrecht, Netherlands.
35. Samson R.A., Houbraken J.A.M.P., Kuijpers A.F.A., Frank J.M., Frisvad J.C. New ochratoxin A or sclerotium producing species of Aspergillus section Nigri. Studies in Mycology 2004, 50:45-61.
36. Sanchez-Hervas M., Gil J.V., Bisbal F., Ramon D., Martínez-Culebras P.V. Mycobiota and mycotoxin producing fungi from cocoa beans. International Journal of Food Microbiology 2008, 125:336-340.
37. Tafuri A., Ferracane R., Ritieni A. Ochratoxin A in Italian marketed cocoa products. Food Chemistry 2004, 88:487-494.
38. Taniwaki M.H., Pitt J.I., Teixeira A.A., Iamanaka B.T. The source of ochratoxin A in Brazilian coffee and its formation in relation to processing methods. International Journal of Food Microbiology 2003, 82:173-179.
39. Van der Merwe K.J., Steyne P.S., Fourie L.F., Scott D.B., Theron J.J. Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus Wilh. Nature 1965, 205:1112-1113.
40. WHO (World Health Organization) Safety Evaluation of Certain Mycotoxins in Food. Prepared by the Fifty-Sixth Meeting of the Joint FAO/WHO Expert Committee on Food Additives 2001, 27-35. World Health Organization, Geneva.