JefA (Rv2459), a drug efflux gene in Mycobacterium tuberculosis confers resistance to isoniazid & ethambutol

Indian Journal of Medical Research

Volumn 132, Issue 8, 2010, Pages 176-188

  Gupta, Anuj Kumar ^a   Reddy, Vineel P ^b   Lavania, Mallika ^a   Chauhan, D S ^a   Venkatesan, K ^a   Sharma, V D ^a   Tyagi, A K ^b   Katoch, V M ^a,c,d  

^a NATIONAL JALMA INSTITUTE FOR LEPROSY AND OTHER MYCOBACTERIAL DISEASES   (India)

^b UNIVERSITY OF DELHI   (India)

^c GOVERNMENT OF INDIA   (India)

^d INDIAN COUNCIL OF MEDICAL RESEARCH   (India)

Author keywords

Drug efflux; Ethambutol; Isoniazid; jefA; Mycobacterium tuberculosis

Indexed keywords

CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; DNA; ETHAMBUTOL; ISONIAZID; RESAZURIN; RNA; VERAPAMIL; BACTERIAL PROTEIN; CARRIER PROTEIN; PRIMER DNA;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DNA SEQUENCE; DRUG SPECIFICITY; DRUG TRANSPORT; ELECTROPORATION; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE FUNCTION; GENE OVEREXPRESSION; JEFA; MICROTITER PLATE ASSAY; MINIMUM INHIBITORY CONCENTRATION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; OPEN READING FRAME; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; AMINO ACID SEQUENCE; BIOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; CLUSTER ANALYSIS; GENETICS; MICROBIOLOGICAL EXAMINATION; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; PHYSIOLOGY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; CLUSTER ANALYSIS; COMPUTATIONAL BIOLOGY; DNA PRIMERS; DRUG RESISTANCE, MICROBIAL; ETHAMBUTOL; ISONIAZID; MEMBRANE TRANSPORT PROTEINS; MICROBIAL SENSITIVITY TESTS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM TUBERCULOSIS; OPEN READING FRAMES; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 77956068391     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Piddock LJ. Multidrug resistance efflux pumps - not just for resistance. Nat Rev Microbiol 2006; 4: 629-36.
2. Webber MA, Piddock LJV. The importance of efflux pumps in bacterial antibiotic resistance. J Antimicrob Chemother 2003; 51: 9-11.
3. Putman M, vanVeen HW, Konings WN. Molecular properties of bacterial multidrug transporters. Microbiol Mol Biol Rev 2000; 64: 672-93.
4. Juliano RL, Ling V. A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. Biochim Biophys Acta 1976; 455: 152-62.
5. Kuo MT. Roles of multidrug resistance genes in breast cancer chemoresistance. Adv Exp Med Biol 2007; 608: 23-30.
6. Poole K. Efflux mediated antimicrobial resistance. J Antimicrob Chemother 2005; 56: 20-51.
7. Centers for Disease Control and Prevention (CDC). Revised defnition of extensively drug-resistant tuberculosis. MMWR Morb Mortal Wkly Rep 2006; 55: 1176.
8. DeRossi E, Branzoni M, Cantoni R, Milano A, Riccardi G, Ciferri O. mmr, a Mycobacterium tuberculosis gene conferring resistance to small cationic dyes and inhibitors. J Bacteriol 1998; 180: 6068-71.
9. De Rossi E, Ainsa JA, Riccardi G. Role of mycobacterial efflux transporters in drug resistance: an unresolved question. FEMS Microbiol Rev 2006; 30: 36-52.
10. Ainsa JA, Blokpoel MC, Otal I, Young DB, De Smet KA, Martin C. Molecular cloning and characterization of Tap, a putative multidrug efflux pump present in Mycobacterium fortuitum and Mycobacterium tuberculosis. J Bacteriol 1998; 180: 5836-43.
11. Choudhuri BS, Bhakta S, Barik R, Basu J, Kundu M, Chakrabarti P. Overexpression and functional characterization of an ABC (ATP-binding cassette) transporter encoded by the genes drrA and drrB of Mycobacterium tuberculosis. Biochem J 2002; 367: 279-85.
12. Siddiqi N, Das R, Pathak N, Banerjee S, Ahmed N, Katoch VM, et al. Mycobacterium tuberculosis isolate with a distinct genomic identity overexpresses a tap-like efflux pump. Infection 2004; 32: 109-11.
13. Colangeli R, Helb D, Sridharan S, Sun J, Varma-Basil M, Hazbon MH, et al. The Mycobacterium tuberculosis iniA gene is essential for activity of an efflux pump that confers drug tolerance to both isoniazid and ethambutol. Mol Microbiol 2005; 55: 1829-40.
14. Pasca MR, Guglierame P, De Rossi E, Zara F, Riccardi G. MmpL7 gene of Mycobacterium tuberculosis is responsible for isoniazid efflux in Mycobacterium smegmatis. Antimicrob Agents Chemother 2005; 49: 4775-7.
15. Pasca MR, Guglierame P, Arcesi F, Bellinzoni M, De Rossi E, Riccardi G. Rv2686c-Rv2687c-Rv2688c, an ABC fuoroquinolone efflux pump in Mycobacterium tuberculosis. Antimicrob Agents Chemother 2004; 48: 3175-80.
16. Gupta AK, Chauhan DS, Srivastava K, Das R, Batra S, Mittal M, et al. Estimation of efflux mediated multi-drug resistance and its correlation with expression levels of two major efflux pumps in mycobacteria. J Commun Dis 2006; 38: 246-54.
17. Ramón-García S, Martín C, DeRossi E, Aínsa JA. Contribution of the Rv2333c efflux pump (the Stp protein) from Mycobacterium tuberculosis to intrinsic antibiotic resistance in Mycobacterium bovis BCG. J Antimicrob Chemother 2007; 59: 544-7.
18. Gupta AK, Katoch VM, Chauhan DS, Sharma R, Singh M, Venkatesan K, et al. Microarray analysis of efflux pump genes in multidrug resistant Mycobacterium tuberculosis during stress induced by common anti-tuberculous drugs. Microb Drug Resist 2010; 16: 21-8.
19. Bullock WO, Fernandez JM, Short JM. XL1-Blue: A high effciency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques 1987; 5: 376-8.
20. DasGupta SK, Jain S, Kaushal D, Tyagi AK. Expression systems for study of mycobacterial gene regulation and development of recombinant BCG vaccines. Biochem Biophys Res Commun 1998; 246: 797-804.
21. Rozen S, Skaletsky HJ. Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S, editors. Bioinformatics methods and protocols: Methods in molecular biology. Totowa, NJ: Humana Press; 2000. p. 365-86. Available at: http://fokker.wi.mit.edu/primer3/input.htm.
22. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods 2001; 25: 402-8.
23. Palomino JC, Martin A, Camacho M, Guerra H, Swings J, Portaels F. Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother 2002; 46: 2720-2.
24. Martin A, Camacho M, Portaels F, Palomino JC. Resazurin microtitre assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple and inexpensive method. Antimicrob Agents Chemother 2003; 47: 3616-9.
25. Nateche F, Martin A, Baraka S, Palomino JC, Khaled S, Portaels F. Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers. J Med Microbiol 2006; 55: 857-60.
26. Jadaun GP, Agarwal C, Sharma H, Ahmed Z, Upadhyay P, Faujdar J, et al. Determination of ethambutol MICs for Mycobacterium tuberculosis and M.avium isolates by resazurin microtitre assay. J Antimicrob Chemother 2007; 60: 152-5.
27. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215: 403-10.
28. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specifc gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22: 4673-80.
29. Hofmann K, Stoffel W. TMbase- A database of membrane spanning protein segments. Biol Chem Hoppe-Seyler 1993; 374: 166-70.
30. Liu J, Takiff HE, Nikaido H. Active efflux of fuoroquinolones in Mycobacterium smegmatis, mediated by LfrA, a multidrug efflux pump. J Bacteriol 1996; 178: 3791-5.
31. Takiff HE, Cimino M, Musso MC, Weisbrod T, Martinez R, Delgado MB, et al. Efflux pump of the proton antiporter family confers low-level fuoroquinolone resistance in Mycobacterium smegmatis. Proc Natl Acad Sci USA 1996; 93: 362-6.
32. Silva PE, Bigi F, Santangelo MP, Romano MI, Martin C, Cataldi A, et al. Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis. Antimicrob Agents Chemother 2001; 45: 800-4.
33. Ahmad S, Mokaddas E. Contribution of AGC to ACC and other mutations at codon 315 of the katG gene in isoniazid-resistant Mycobacterium tuberculosis isolates from the Middle East. Int J Antimicrob Agents 2004; 23: 473-9.
34. Ahmad S, Jaber AA, Mokaddas E. Frequency of embB codon 306 mutations in ethambutol-susceptible and -resistant clinical Mycobacterium tuberculosis isolates in Kuwait. Tuberculosis (Edinb) 2007; 87: 123-9.
35. Hazbon MH, Bobadilla delValle M, Guerrero MI, Varma-Basil M, Filliol I, Cavatore M, et al. Role of embB codon 306 mutations in Mycobacterium tuberculosis revisited: a novel association with broad drug resistance and IS6110 clustering rather than ethambutol resistance. Antimicrob Agents Chemother 2005; 49: 3794-802.
36. Parsons LM, Salfnger M, Clobridge A, Dormandy J, Mirabello L, Polletta VL, et al. Phenotypic and molecular characterization of Mycobacterium tuberculosis isolates resistant to both isoniazid and ethambutol. Antimicrob Agents Chemother 2005; 49: 2218-25.
37. Plinke C, Rusch-Gerdes S, Niemann S. Signifcance of mutations in embB codon 306 for prediction of ethambutol resistance in clinical Mycobacterium tuberculosis isolates. Antimicrob Agents Chemother 2006; 50: 1900-2.
38. Fu LM. Exploring drug action on Mycobacterium tuberculosis using Affymetrix oligonucleotide genechips. Tuberculosis (Edinb) 2006; 86: 134-43.
39. Wilson M, DeRisi J, Kristensen HH, Imboden P, Rane S, Brown PO, et al. Exploring drug-induced alterations in gene expression in Mycobacterium tuberculosis by microarray hybridization. Proc Natl Acad Sci USA 1999; 96: 12833-8.
40. Guex N, Peitsch MC. SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling. Electrophoresis 1997; 18: 2714-23.