New insights into the de novo gene synthesis using the automatic kinetics switch approach

Analytical Biochemistry

Volumn 406, Issue 1, 2010, Pages 51-60

  Cheong, Wai Chye ^a   Lim, Li Shi ^a   Huang, Mo Chao ^a   Bode, Marcus ^a   Li, Mo Huang ^a  

^a INSTITUTE OF BIOENGINEERING AND NANOTECHNOLOGY   (Singapore)

Author keywords

Assembly efficiency; Automatic kinetics switch; De novo gene synthesis; LCGreen; Multiplex gene synthesis; PCR; Real time gene synthesis

Indexed keywords

BIOSYNTHESIS; DNA; GENES; KINETICS; OLIGONUCLEOTIDES; THERMODYNAMICS;

ANNEALING TEMPERATURES; ANNEALING TIME; DNA HYBRIDIZATION; GENE ASSEMBLY; GENE SYNTHESIS; LCGREEN; PCR AMPLIFICATION; THERMODYNAMICS AND KINETICS;

POLYMERASE CHAIN REACTION;

CALVASCULIN; DNA; OLIGONUCLEOTIDE;

ARTICLE; DNA HYBRIDIZATION; GENE; GENE SYNTHESIS; KINETICS; LUC 1 GENE; LUC 2 GENE; OR17 GENE; PKB2 GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SUGE GENE; THERMODYNAMICS; YFIK GENE;

EID: 77955842604     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.06.036     Document Type: Article

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