Accurate multiplex gene synthesis from programmable DNA microchips

Nature

Volumn 432, Issue 7020, 2004, Pages 1050-1054

  Tian, Jingdong ^a   Gang, Hui ^a   Sheng, Nijing ^b   Zhou, Xiaochuan ^c   Gulari, Erdogan ^d   Gao, Xiaolian ^b   Church, George ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b University of Houston   (United States)

^c Atactic Technologies Inc   (United States)

^d UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COST EFFECTIVENESS; GENES; MICROPROCESSOR CHIPS; MULTIPLEXING; PROTEINS; SYNTHESIS (CHEMICAL);

CODON BIAS; HYBRIDIZATION; MICROFLUIDIC CHIPS; RIBOSOMES;

DNA;

GENE;

ARTICLE; CODON; DNA MICROARRAY; GENE DELETION; GENETIC COMPLEMENTATION; GENOME ANALYSIS; GENOMICS; HYPOTHESIS; NUCLEOTIDE METABOLISM; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RIBOSOME SUBUNIT;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES; GENOMICS; MICROFLUIDICS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OLIGONUCLEOTIDES; PROTEIN BIOSYNTHESIS; PROTEIN SUBUNITS; RESEARCH DESIGN; RIBOSOMAL PROTEINS; RIBOSOMES; SENSITIVITY AND SPECIFICITY; SOFTWARE;

ESCHERICHIA COLI;

EID: 11144247743     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature03151     Document Type: Article

References (21)

1. Zhou, X. et al. Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences. Nucleic Acids Res. 32, 5409-5417 (2004).
2. Fodor, S. P. et al. Light-directed, spatially addressable parallel chemical synthesis. Science 251, 767-773 (1991).
3. Gao, X. et al. Flexible DNA chip synthesis gated by deprotection using solution photogenerated acids. Nucleic Acids Res. 29, 4744-4750 (2001).
4. Cello, J., Paul, A. V. & Wimmer, E. Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template. Science 297, 1016-1018 (2002).
5. Smith, H. O., Hutchison, C. A. III, Pfannkoch, C. & Venter, J. C. Generating a synthetic genome by whole genome assembly: ΦX174 bacteriophage from synthetic oligonucleotides. Proc. Natl Acad. Sci. USA 100, 15440-75445 (2003).
6. Stemmer, W. P., Crameri, A., Ha, K. D., Brennan, T. M. & Heyrseker, H. L. Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene 164, 49-53 (1995).
7. Eason, R. G. et al. Characterization of synthetic DNA bar codes in Saccharomyces cerevisiae gene-deletion strains. Proc. Natl Acad. Sci. USA 101, 11046-11051 (2004).
8. Forster, A. C. & Church, G. M. A synthetic biology project. Nature (submitted).
9. Culver, G. M. & Noller, H. F. Efficient reconstitution of functional Escherichia coli 30S ribosomal subunits from a complete set of recombinant small subunit ribosomal proteins. RNA 5, 832-843 (1999).
10. Iost, I., Guillerez, J. & Dreyfus, M. Bacteriophage T7 RNA polymerase travels far ahead of ribosomes in vivo. J. Bacteriol. 174, 619-622 (1992).
11. Iost, I. & Dreyfus, M. mRNAs can be stabilized by DEAD-box proteins. Nature 372, 193-196 (1994).
12. Carr, P. A., Park, J. S., Lee, Y.-J., Yu, T., Zhang, S. & Jacobson, J. M. Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res. (in the press).
13. Smith, J. & Modrich, P. Removal of polymerase-produced mutant sequences from PCR products. Proc. Natl Acad. Sci. USA 94, 6847-6850 (1997).
14. Shendure, J., Mitra, R., Varma, C. & Church, G. M. Advanced sequencing technologies: methods and goals. Nature Rev. Genet. 5, 335-344 (2004).
15. Mullis, K. et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb. Symp. Quant. Biol. LI, 263-273 (1986).
16. Dillon, P. J. & Rosen, C. A. A rapid method for the construction of synthetic genes using the polymerase chain reaction. BioTechniques 9, 298-300 (1990).
17. Liu, D., Park, S. H., Reif, J. H. & LaBean, T. H. DNA nanotubes self-assembled from triple-crossover tiles as templates for conductive nanowires. Proc. Natl Acad. Sci. USA 101, 717-722 (2004).
18. Jaffe, J. D., Berg, H. C. & Church, G. M. Proteogenomic mapping reveals genomic structure and novel proteins undetected by computational algorithms. Proteomics 4, 59-77 (2004).
19. Breslauer, K. J., Frank, R., Blöcker, H. & Marky, L. A. Predicting DNA duplex stability from the base sequence. Proc. Natl Acad. Sci. USA 83, 3746-3750 (1986).
20. Richmond, K. E. et al. Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis. Nucleic Acids Res. 32, 5011-5018 (2004).
21. Espelund, M., Stacy, R. A. & Jakobsen, K. S. A simple method for generating single-stranded DNA probes labeled to high activities. Nucleic Acids Res. 18, 6157-6158 (1990).