Structurally informed site-directed mutagenesis of a stereochemically promiscuous aldolase to afford stereochemically complementary biocatalysts

Journal of the American Chemical Society

Volumn 132, Issue 33, 2010, Pages 11753-11758

  Royer, Sylvain F ^a   Haslett, Luke ^a   Crennell, Susan J ^a   Hough, David W ^a   Danson, Michael J ^a   Bull, Steven D ^b  

^a University of Bath   (United Kingdom)

^b University of Bath   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALDOL REACTIONS; ALDOLASES; CARBON-CARBON BOND FORMATION; DIASTEREOCONTROL; HYPERTHERMOPHILES; NATURAL SUBSTRATES; PYRUVATES; SITE DIRECTED MUTAGENESIS; STRUCTURAL INFORMATION; SULFOLOBUS SOLFATARICUS;

AMINO ACIDS; CHEMICAL REACTIONS; ORGANIC ACIDS;

SUBSTRATES;

AMINO ACID; CARBON; DEXTRO 2 KETO 3 DEOXY GALACTONATE; DEXTRO 2 KETO 3 DEOXYGLUCONATE; FRUCTOSE BISPHOSPHATE ALDOLASE; GALACTOSE; GLUCOSE DERIVATIVE; UNCLASSIFIED DRUG;

ALDOL REACTION; ARTICLE; BIOCATALYST; CATALYSIS; CHEMICAL BOND; CRYSTALLOGRAPHY; HYPERTHERMOPHILE; INFORMATION; MUTANT; NONHUMAN; SITE DIRECTED MUTAGENESIS; STEREOCHEMISTRY; SULFOLOBUS SOLFATARICUS; X RAY;

ALDEHYDE-LYASES; BIOCATALYSIS; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; STEREOISOMERISM; SULFOLOBUS SOLFATARICUS;

EID: 77955830263     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja104412a     Document Type: Article

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