Housekeeping gene stability influences the quantification of osteogenic markers during stem cell differentiation to the osteogenic lineage

Cytotechnology

Volumn 62, Issue 2, 2010, Pages 109-120

  Quiroz, Felipe Garcia ^a,e   Posada, Olga M ^a   Gallego Perez, Daniel ^b   Higuita Castro, Natalia ^a,b   Sarassa, Carlos ^c   Hansford, Derek J ^b   Agudelo Florez, Piedad ^d   López, Luis E ^a  

^a UNIVERSIDAD CES   (Colombia)

^b Ohio State University   (United States)

^c HOSPITAL PABLO TOBÓN URIBE   (Colombia)

^d Instituto Colombiano de Medicina Tropical   (Colombia)

^e NONE   (United States)

Author keywords

Gene expression; Mesenchymal stem cells; Normalization; Osteogenic differentiation; Quantitative RT PCR

Indexed keywords

ALIZARIN RED S; BETA ACTIN; BONE SIALOPROTEIN; COLLAGEN TYPE 1; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; OSTEONECTIN; RIBOSOME PROTEIN; RIBOSOME PROTEIN L13A; UNCLASSIFIED DRUG;

ARTICLE; BONE DEVELOPMENT; BONE MARROW CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL LINEAGE; CONTROLLED STUDY; GENE ACTIVITY; GENE EXPRESSION; GENETIC STABILITY; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; MORPHOLOGICAL TRAIT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; STAINING; UPREGULATION;

EID: 77955709540     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-010-9265-1     Document Type: Article

References (43)

1. Abdallah BM, Haack-Sorensen M, Burns JS, Elsnab B, Jakob F, Hokland P, Kassem M (2005) Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene in despite of extensive proliferation. Biochem Biophys Res Commun 326:527-538
2. Aerts JL, Gonzales MI, Topalian SL (2004) Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR. BioTechniques 36:84-91
3. Andersen CL, Jensen JL, Ørntoft TF (2004) Normalization of real-time quantitative reverse transcription-pcr data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets cancer research. Cancer Res 64: 5245-5250
4. Bianco P, Riminuncci M, Gronthos S, Robey PG (2001) Bone marrow stromal stem cells: Nature, biology, and potential applications. Stem Cells 19:180-192
5. Biederman J, Yee J, Cortes P (2004) Validation of internal control genes for gene expression analysis in diabetic glomerulosclerosis. Kidney Int 66:2308-2314
6. Bustin S (2000) Absolute quantification of mRNA using realtime reverse transcription polymerase chain reaction assay. J Mol Endocrinol 25:169-193
7. Catelas I, Sese N, Wu BM, Dunn JCY, Helgerson S, Tawil B (2006) Human mesenchymal stem cell proliferation and osteogenic differentiation in fibrin gels in vitro. Tissue Eng 11:2385-2396
8. Caterson EJ, Nesti LJ, Danielson KG et al (2002) Human marrow-derived mesenchymal progenitor cells:Isolation, culture expansion, and analysis of differentiation. Mol Biotechnol 20:245-256
9. Cho HH, Park HT, Kim YJ, Bae YC, Suh KT, Jung JS (2005) Induction of osteogenic differentiation of human mesenchymal stem cells by histone deacetylase inhibitors. J Cell Biochem 96:533-542
10. Colter DC, Sekiya I, Prockop DJ (2001) Identification of a subpopulation of rapidly selfrenewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci USA 98:7841-7845
11. Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A (2004) Validation of housekeeping genes for normalizing RNA expression in real-time PCR. BioTechniques 37:112-119
12. Frank O, Heim M, Jakob M, Barbero A, Schäfer D, Bendik I, Dick W, Heberer M, Martin I (2002) Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro. J Cell Biochem 85:737-746
13. Friedman MS, Long MW, Hakenson KD (2006) Osteogenic differentiation of human mesenchymal stem cells is regulated by bone morphogenetic protein-6. J Cell Biochem 98:538-554
14. García-Crespo D, Juste RA, Hurtado A (2005) Selection of ovine housekeeping genes for normalisation by real-time RT-PCR; analysis of PrP gene expression and genetic susceptibility to scrapie. BMC Vet Res 1:3
15. Glare EM, Divjak M, Bailey MJ, Walters EH (2002) β-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels. Thorax 57:765-770
16. Goossens K, Poucke MV, Soom AV, Vandesompele J, Van Zeveren A, Peelman LC (2005) Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos. BMC Dev Biol 5:27
17. Grayson WL, Ma T, Bunnell B (2004) Human mesenchymal stem cells tissue development in 3D PET matrices. Biotechnol Prog 20:905-912
18. Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8(2):R19
19. Huggett J, Dheda K, Bustin S, Zumla A (2005) Real-time RTPCR normalisation; strategies and considerations. Genes Immun 6:279-284
20. Johansson S, Fuchs A, Ö kvis A, Karimi M, Harper C, Garrick T, Sheedy D, Hurd Y, Bakalkin G, Ekström TJ (2007) Validation of endogenous controls for quantitative gene expression analysis: Application on brain cortices of human chronic alcoholics. Brain Res 1132:20-28
21. Kim HJ, Kim UJ, Vunjak-Novakovic G, Min BH, Kaplan DL (2005) Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells. Biomaterials 26:4442-4452
22. Kleiboeker SB (2005) Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR. Virol J 2:29
23. Kok JB, Roelofs RW, Giesendorf BA, Pennings JL, Waas ET, Feuth T, Swinkels DW, Span PN (2005) Normalization of gene expression measurements in tumor tissues: Comparison of 13 endogenous control genes. Lab Invest 85:154-159
24. Lee H, Huang G, Chiang H, Chiou LL, Chen MH, Hsieh CH, Jiang CH (2003) Multipotential mesenchymal stem cells from femoral bone marrow near the site of osteonecrosis. Stem Cells 21:190-199
25. Mbalaviele G, Sheikh S, Stains JP, Salazar VS, Cheng SL, Chen D, Civitelli R (2005) b-Catenin and BMP-2 synergize to promote osteoblast differentiation and new bone formation. J Cell Biochem 94:403-418
26. Meinel L, Karageorgiou V, Hofmann S, Fajardo R, Snyder B, Li C, Zichner L, Langer R, Vunjak-Novakovic G, Kaplan DL (2004a) Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. J Biomed Mater Resv 71A:25-34 (Pubitemid 39244392)
27. Meinel L, Karageorgiou V, Fajardo R, Snyder B, Shinde-Patil V, Zichner L, Kaplan D, Langer R, Vunjak-Novakovic G (2004b) Bone tissue engineering using human mesenchymal stem cells: Effects of scaffold material and medium flow. Ann Biomed 32:112-122
28. Meinel L, Hofmann S, Betz O, Fajardo R, Merkle HP, Langer R, Evans CH, Vunjak-Novakovic G, Kaplan DL (2006) Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials: Comparison of adenovirus mediated gene transfer and protein delivery of BMP-2. Biomaterials 27:4993-5002
29. Mygind T, Stiehler M, Baatrup A, Li H, Zou X, Flyvbjerg A, Kassem M, Bünger C (2007) Mesenchymal stem cell ingrowth and differentiation on coralline hydroxyapatite scaffolds. Biomaterials 28:1036-1047 (Pubitemid 44809415)
30. Oshina H, Sotome S, Yoshii T, Torigoe I, Yumi S, Maehara H, Marukawa E, Omura K, Shinomiya K (2007) Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Bone 41:575-583
31. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: bestkeeper- excel-based tool using pair-wise correlations. Biotechnol Lett 26:509-515
32. Quiroz GF, Posada OM, Gallego D, Higuita N, Sarassa C, Hansford DJ, Agudelo-Florez P, López LE (2008) Isolation of human bone marrow mesenchymal stem cells and evaluation of their osteogenic potential. Revista Ingeniería Biomédica 3:48-55
33. Ramakers C, Ruijter JM, Lekanne Deprez RH, Moorman AFM (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett 339:62-66
34. Sakaguchi Y, Sekiya I, Yagishita K, Ichinose S, Shinomiya K, Muneta T (2004) Suspended cells from trabecular bone by collagenase digestion become virtually identical to mesenchymal stem cells obtained from marrow aspirates. Blood 104:2728-2735
35. Schmittgen T, Zakrajsek B (2000) Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods 46:69-81
36. Schutze N, Noth U, Schneidereit J, Hendrich C, Jakob F (2005) Differential expression of CCN-family members in primary human bone marrow-derived mesenchymal stem cells during osteogenic, chondrogenic and adipogenic differentiation. Cell Commun Signal 3:5
37. Sumanasinghe RD, Bernacki SH, Loboa EG (2006) Osteogenic differentiation of human mesenchymal stem cells in collagen matrices: Effect of uniaxial cyclic tensile strain on bone morphogenetic protein (BMP-2) mRNA expression. Tissue Eng 12:3459-3465
38. Szabo A, Perou CM, Karaca M, Perreard L, Palais R, Quackenbush JF, Bernard PS (2004) Statistical modeling for selecting housekeeper genes. Genome Biol 5:R59
39. Tricarico C, Pinzani P, Bianchi S, Paglierani M, Distante V, Pazzagli M, Bustin SA, Orlando C (2002) Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem 309:293-300
40. Tsukahara S, Ikeda R, Goto S, Yoshida K, Mitsumori R, Sakamoto Y, Tajima A, Yokoyama T, Toh S, Furukawa KI, Inoue I (2006) Tumour necrosis factor a-stimulated gene-6 inhibits osteoblastic differentiation of human mesenchymal stem cells induced by osteogenic differentiation medium and BMP-2. Biochem J 398:595-603
41. Vandesompele J, De preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3(7):research0034.1-0034.11
42. Wong ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. BioTechniques 39:75-85
43. Zhang X, Ding L, Sandford AJ (2005) Selection of reference genes for gene expression studies in human neutrophils by real-time PCR. BMC Mol Biol 6:4