Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds

Journal of Biomedical Materials Research - Part A

Volumn 71, Issue 1, 2004, Pages 25-34

  Meinel, Lorenz ^a,b,c   Karageorgiou, Vassilis ^c   Hofmann, Sandra ^c,d   Fajardo, Robert ^e   Snyder, Brian ^e   Li, Chunmei ^c   Zichner, Ludwig ^b   Langer, Robert ^a   Vunjak Novakovic, Gordana ^a   Kaplan, David L ^c  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b UNIVERSITY HOSPITAL FRANKFURT   (Germany)

^c Tufts University   (United States)

^d ETH ZURICH   (Switzerland)

^e HARVARD UNIVERSITY   (United States)

Author keywords

Hydroxyapatite; Osteogenic; Silk; Stem cells; Tissue engineering

Indexed keywords

BONE; CELLS; COLLAGEN; DEGRADATION; MICROSTRUCTURE; PROTEINS; TISSUE; X RAY DIFFRACTION ANALYSIS;

BIODEGRADABLE SILK SCAFFOLDS; HISTOLOGICAL ANALYSIS; HUMAN BONE MARROW; OSTEOGENIC DIFFERENTIATION; SLOW DEGRADATION;

BIOMEDICAL ENGINEERING;

ARGINYLGLYCYLASPARTIC ACID; BIOCHEMICAL MARKER; BONE MORPHOGENETIC PROTEIN 2; COLLAGEN; HYDROXYAPATITE; OSTEOPONTIN; SIALOPROTEIN;

ARTICLE; BIODEGRADATION; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CHEMICAL ANALYSIS; CHEMICAL STRUCTURE; COMPUTER ASSISTED TOMOGRAPHY; CONTROLLED STUDY; COVALENT BOND; HEMATOPOIETIC STEM CELL; HISTOLOGY; HUMAN; HUMAN CELL; IN VITRO STUDY; MESENCHYME CELL; MINERALIZATION; POROSITY; PROTEIN EXPRESSION; SILK; TISSUE ENGINEERING; X RAY DIFFRACTION;

ABSORBABLE IMPLANTS; ANIMALS; BIOCOMPATIBLE MATERIALS; BONE AND BONES; BONE MARROW CELLS; BONE MORPHOGENETIC PROTEINS; CELL DIFFERENTIATION; CELLS, CULTURED; HUMANS; HYDROXYAPATITES; MESENCHYMAL STEM CELLS; OSTEOPONTIN; PEPTIDES; SIALOGLYCOPROTEINS; SILK; TISSUE ENGINEERING; TRANSFORMING GROWTH FACTOR BETA;

EID: 4544323251     PISSN: 00219304     EISSN: None     Source Type: Journal    
DOI: 10.1002/jbm.a.30117     Document Type: Article

References (45)

1. Lysaght MJ, Nguy NA, Sullivan K. An economic survey of the emerging tissue engineering industry. Tissue Eng 1998;4:231-238.
2. Vacanti JP, Langer R. Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation. Lancet 1999;354 (Suppl 1):S132-S134.
3. Friedenstein AJ. Precursor cells of mechanocytes. Int Rev Cytol 1976;47:327-359.
4. Friedenstein AJ, Chailakhyan RK, Gerasimov UV. Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet 1987;20:263-272.
5. Caplan AI. The mesengenic process. Clin Plast Surg 1994;21:429-435.
6. Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng 1998;4:415-428.
7. Ohgushi H, Okumura M, Masuhara K, Goldberg VM, Davy DT, Caplan AI. Calcium phosphate block ceramic with bone marrow cells in a rat long bone defect. In: Yamamuro T, Hench LL, Wilson J, editors. CRC handbook of bioactive ceramics. Vol. 2. Boca Raton, FL: CRC Press; 1992. pp 235-238.
8. Petite H, Viateau V, Bensaid W, Meunier A, de Pollak C, Bourguignon M, Oudina K, Sedel L, Guillemin G. Tissue-engineered bone regeneration. Nat Biotechnol 2000;18:959-963.
9. Athanasiou KA, Niederauer GG, Agrawal CM. Sterilization, toxicity, biocompatibility and clinical applications of polylactic acid/polyglycolic acid copolymers. Biomaterials 1996;17:93-102.
10. Hollinger JO, Brekke J, Gruskin E, Lee D. Role of bone substitutes. Clin Orthop 1996;324:55-65.
11. Harris LD, Kim BS, Mooney DJ. Open pore biodegradable matrices formed with gas foaming. J Biomed Mater Res 1998;42:396-402.
12. Suh H. Recent advances in biomaterials. Yonsei Med J 1998;39:87-96.
13. Sofia S, McCarthy MB, Gronowicz G, Kaplan DL. Functionalized silk-based biomaterials for bone formation. J Biomed Mater Res 2001;54:139-148.
14. Lange F. Über die Sehenplastik. Verh Dtsch Orthop Ges 1903;2:10-12.
15. Lange F. Künstliche Bänder aus Seide. Münch Med Wochenschr 1907;17:834-836.
16. Perez-Rigueiro J, Viney C, Llorca J, Elices M. Silkworm silk as an engineering material. J Appl Poly Sci 1998;70:2439-2447.
17. Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL. Silk-based biomaterials. Biomaterials 2003;24:401-416.
18. Altman GH, Horan RL, Lu HH, Moreau J, Martin I, Richmond JC, Kaplan DL. Silk matrix for tissue engineered anterior cruciate ligaments. Biomaterials 2002;23:4131-4141.
19. Kaplan DL. Spiderless spider webs. Nat Biotechnol 2002;20:239-240.
20. Chen J, Altman GH, Karageorgiou V, Horan RL, Collette A, Volloch V, Colabro T, Kaplan DL. Human bone marrow stromal cells and ligament fibroblasts responses on RGD-modified silk fibres. J Biomed Mater Res 2003;67A:559-570.
21. Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y. Attachment and growth of cultured fibroblast cells on silk protein matrices. J Biomed Mater Res 1995;29:1215-1221.
22. Nazarov C, Jin HJ, Kaplan DL. Porous 3-d scaffolds from regenerated silk fibroin. Biomacromolecules 2004;5:718-726.
23. Meinel L, Kareourgiou V, Fajardo R, Snyder B, Shinde-Patil V, Zichner L, Kaplan D, Langer R, Vunjak-Novakovic G. Bone tissue engineering using human mesenchymal stem cells: effects of scaffold material and medium flow. Ann Biomed Eng 2003;32:112-122.
24. Meinel L, Hofmann S, Karageorgiou V, Kirker-Head C, Zichner L, Langer R, Vunjak-Novakovic G, Kaplan D. Inflammatory responses in vivo and in vitro to silk and collagen films. Biomaterials 2003. Forthcoming.
25. Martin I, Obradovic B, Freed LE, Vunjak-Novakovic G. Method for quantitative analysis of glycosaminoglycan distribution in cultured natural and engineered cartilage. Ann Biomed Eng 1999;27:656-662.
26. Freed LE, Marquis JC, Nohria A, Emmanual J, Mikos AG, Langer R. Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res 1993;27:11-23.
27. Farndale RW, Buttle DJ, Barrett AJ. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 1986;883:173-177.
28. Rosenberg L. Chemical basis for the histological use of safranin O in the study of articular cartilage. J Bone Joint Surg Am 1971;53:69-82.
29. Sheehan DC, Hrapchak BB. Theory and practice of histotechnology. St. Louis, MO: C. V. Mosby Co.; 1973. pp xi, 218.
30. Muller R, Hildebrand T, Ruegsegger P. Non-invasive bone biopsy: a new method to analyse and display the three-dimensional structure of trabecular bone. Phys Med Biol 1994;39:145-164.
31. Harper RA, Posner AS. Measurement of non-crystalline calcium phosphate in bone mineral. Proc Soc Exp Biol Med 1966;122:137-142.
32. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143-147.
33. Banfi A, Bianchi G, Notaro R, Luzzatto L, Cancedda R, Quarto R. Replicative aging and gene;Expression in long-term cultures of human bone marrow stromal cells. Tissue Eng 2002;8:901-910.
34. Barry FP, Boynton RE, Haynesworth S, Murphy JM, Zaia J. The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105). Biochem Biophys Res Commun 1999;265:134-139.
35. van Luyn MJ, van Wachem PB, Damink LO, Dijkstra PJ, Feijen J, Nieuwenhuis P. Relations between in vitro cytotoxicity and crosslinked dermal sheep collagens. J Biomed Mater Res 1992;26:1091-1110.
36. Ishaug SL, Crane GM, Miller MJ, Yasko AW, Yaszemski MJ, Mikos AG. Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds. J Biomed Mater Res 1997;36:17-28.
37. Martin I, Shastri VP, Padera RF, Yang J, Mackay AJ, Langer R, Vunjak-Novakovic G, Freed LE. Selective differentiation of mammalian bone marrow stromal cells cultured on three-dimensional polymer foams. J Biomed Mater Res 2001;55:229-235.
38. Freed LE, Vunjak-Novakovic G. Tissue engineering bioreactors. In: Lanza RP, Langer R, Vacanti J, editors. Principles of tissue engineering. San Diego: Academic Press; 2000. pp 143-156.
39. Betts F, Blumenthal NC, Posner AS, Becker GL, Lehninger AL. Atomic structure of intracellular amorphous calcium phosphate deposits. Proc Natl Acad Sci USA 1975;72:2088-2090.
40. Bonar LC, Grynpas MD, Glimcher MJ. Failure to detect crystalline brushite in embryonic chick and bovine bone by X-ray diffraction. J Ultrastruct Res 1984;86:93-99.
41. Grynpas MD, Bonar LC, Glimcher MJ. Failure to detect an amorphous calcium-phosphate solid phase in bone mineral: a radial distribution function study. Calcif Tissue Int 1984;36:291-301.
42. Fisher LW, Whitson SW, Avioli LV, Termine JD. Matrix sialoprotein of developing bone. J Biol Chem 1983;258:12723-12727.
43. Lecanda F, Avioli LV, Cheng SL. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J Cell Biochem 1997;67:386-396.
44. Zhao M, Berry JE, Somerman MJ. Bone morphogenetic protein-2 inhibits differentiation and mineralization of cementoblasts in vitro. J Dent Res 2003;82:23-27.
45. Butler WT. The nature and significance of osteopontin. Connect Tissue Res 1989;23:123-136.