Validation of internal control genes for gene expression analysis in diabetic glomerulosclerosis

Kidney International

Volumn 66, Issue 6, 2004, Pages 2308-2314

  Biederman, Jason ^a   Yee, Jerry ^a   Cortes, Pedro ^a  

^a HENRY FORD HOSPITAL   (United States)

Author keywords

Diabetic nephropathy; Housekeeping genes; Internal control genes; RT PCR

Indexed keywords

BETA 2 MICROGLOBULIN; BETA ACTIN; CYCLOPHILIN A; GLUCOSE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; PHOSPHOLIPASE A2; RIBOSOME PROTEIN; RNA; ACTIN; PHOSPHOLIPASE A;

ARTICLE; CELL CULTURE; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); DIABETIC NEPHROPATHY; GENE CONTROL; GENE EXPRESSION; HOUSEKEEPING GENE; KIDNEY PARENCHYMA; MEASUREMENT; MESANGIUM CELL; NORTHERN BLOTTING; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VALIDATION PROCESS; ANIMAL; COMPUTER PROGRAM; CYTOLOGY; GENE EXPRESSION PROFILING; GENETICS; GLUCOSE BLOOD LEVEL; MALE; MESANGIUM; PHYSIOLOGY; RAT; REPRODUCIBILITY; STANDARD; VALIDATION STUDY; WISTAR RAT;

ACTINS; ANIMALS; BLOOD GLUCOSE; CELLS, CULTURED; DIABETIC NEPHROPATHIES; GENE EXPRESSION PROFILING; GLOMERULAR MESANGIUM; GLUCOSE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; MALE; PHOSPHOLIPASES A; RATS; RATS, WISTAR; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SOFTWARE;

EID: 21644434747     PISSN: 00852538     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1523-1755.2004.66016.x     Document Type: Article

References (23)

1. FUJIMOTO M, MAEZAWA Y, YOKOTE K, et al: Mice lacking Smad3 are protected against streptozotocin-induced diabetic glomerulopathy. Biochem Biophys Res Commun 305:1002-1007, 2003
2. IHM C-G, LEE GSL, NAST CC, et al: Early increased renal procollagen α1(IV) mRNA levels in streptozotocin induced diabetes. Kidney Int 41:768-777, 1992
3. SASSY-PRIGENT C, HEUDES D, MANDET C, et al: Early glomerular macrophage recruitment in stretptozotocin-induced diabetic rats. Diabetes 49:466-475, 2000
4. HILL C, FLYVBJERG A, GRØNBAEK H, et al: The renal expression of transforming growth factor-β isoforms and their receptors in acute and chronic experimental diabetes in rats. Endocrinology 141:1196-1208, 2000
5. KIM SI, HAN DC, LEE HB: Lovastatin inhibits transforming growth factor-β1 expression in diabetic rat glomeruli and cultured rat mesangial cells. J Am Soc Nephrol 11:80-87, 2000
6. KATO S, LUYCKX VA, OTS M, et al: Renin-angiotensin blockade lowers MCP-1 expression in diabetic rats. Kidney Int 56:1037-1048, 1999
7. NAKAMURA T, EBIHARA I, FUKUI M, et al: Effect of specific endothelin receptor A antagonist on mRNA levels for extracellular matrix components and growth factors in diabetic glomeruli. Diabetes 44:895-399, 1995
8. PUGLIESE G, PRICCI F, PESCE C, et al: Early, but not advanced, glomerulopathy is reversed by pancreatic islet transplants in experimental diabetic rats. Diabetes 46:1198-1206, 1997
9. NAKAMURA T, TAKAHASHI T, FUKUI M, et al: Enalapril attenuates increased gene expression of extracellular matrix components in diabetic rats. J Am Soc Nephrol 5:1492-1497, 1995
10. ADLER SG, KANG S-W, FELD S, et al: Can glomerular mRNAs in human type 1 diabetes be used to predict transition from normoalbuminuria to microalbuminuria? Am J Kidney Dis 40:184-188, 2002
11. ADLER SG, KANG S-W, FELD S, et al: Glomerular mRNAs in human type 1 diabetes: biochemical evidence for microalbuminuria as a manifestation of diabetic nephropathy. Kidney Int 60:2330-2336, 2001
12. SCHMID H, COHEN CD, HENGER A, et al: Validation of endogenous controls for gene expression analysis in microdissected human renal biopsies. Kidney Int 64:356-360, 2003
13. VANDESOMPELE J, DE PRETER K, PATTYN F, et al: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:0034.1-0034.11, 2002
14. MURPHY RM, WATT KK, CAMERON-SMITH D, et al: Effects of creatine supplementation on housekeeping genes in human skeletal muscle using real-time RT-PCR. Physiol Genomics 12:163-174, 2003
15. TSUJI N, KAMAGATA C, FURUYA M, et al: Selection of an internal control gene for quantitation of mRNA in colonic tissues. Anticancer Res 22:4173-4178, 2002
16. GORZELNIAK K, JANKE J, ENGELI S, SHARMA AM: Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes. Horm Metab Res 33:625-627, 2001
17. Rio MC, BELLOCQ JP, GAIRARD B, et al: Specific expression of the pS2 gene in subclasses of breast cancers in comparison with expression of the estrogen and progesterone receptors and the oncogene ERBB2. Proc Natl Acad Sci USA 84:9243-9247, 1987
18. BASSET P, BELLOCQ JP, WOLF C, et al: A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas. Nature 348:699-704, 1990
19. KANG S-W, ADLER SG, NAST CC, et al: 12-lipoxygenase is increased in glucose-stimulated mesangial cells and in experimental diabetic nephropathy. Kidney Int 59:1354-1362, 2001
20. SZAMOSFALVI B, CORTES P, ALVIANI R, et al: Putative subunits of the rat mesangial KATP: A type 2B sulfonylurea receptor and an inwardly rectifying K+ channel. Kidney Int 61:1739-1749, 2002
21. NYENGAARD JR, RASCH R: The impact of experimental diabetes mellitus in rats on glomerular capillary number and sizes. Diabetologia 36:189-194, 1993
22. FÉLIERS D, FRANK MA, RILEY DJ: Activation of cyclin D1-cdk4 and cdk4-directed phosphorylation of rb protein in diabetic mesangial hypertrophy. Diabetes 51:3290-3299, 2002
23. kip1 in cultured mouse mesangial cells: Relationship to hypertrophy. Am J Physiol Renal Physiol 273:348-356, 1997