Novel biosynthesis of (R)-ethyl-3-hydroxyglutarate with (R)-enantioselective hydrolysis of racemic ethyl 4-cyano-3-hydroxybutyate by Rhodococcus erythropolis

Applied Microbiology and Biotechnology

Volumn 87, Issue 4, 2010, Pages 1335-1345

  Dong, Hua Ping ^a,b   Liu, Zhi Qiang ^a   Zheng, Yu Guo ^a   Shen, Yin Chu ^a  

^a ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

^b SHAOXING UNIVERSITY   (China)

Author keywords

(R) ethyl 3 hydroxyglutarate; Colorimetric screening method; Enantioselective hydrolysis; Nitrilase; Rhodococcus erythropolis; Rosuvastatin

Indexed keywords

16S RDNA SEQUENCE; ALIPHATIC NITRILES; BIOCATALYTIC REACTION; CHEMICAL STRUCTURE; COLORIMETRIC REACTION; ENANTIOSELECTIVE HYDROLYSIS; ESTER BONDS; HYDROLASES; MICHAELIS CONSTANTS; NITRILASE; NOVEL PRECURSORS; OPTICAL PURITY; RHODOCOCCUS ERYTHROPOLIS; SCREENING METHODS; SIDE CHAINS; SUBSTRATE CONCENTRATIONS; SYNTHESIS ROUTE; WHOLE CELL; YEAST EXTRACTS;

BIOCHEMISTRY; COLOR; COLORIMETRY; CYANIDES; ESTERS; GLUCOSE; HYDROLYSIS; PH EFFECTS;

SYNTHESIS (CHEMICAL);

AMMONIA; COBALT; DNA 16S; ETHYL 3 HYDROXYGLUTARATE; ETHYL 4 CYANO 3 HYDROXYBUTYATE; GLUTARIC ACID DERIVATIVE; HYDROLASE; NITRILE; RIBOSOME DNA; ROSUVASTATIN; UNCLASSIFIED DRUG; ALPHA-HYDROXYGLUTARATE;

BACTERIUM; BIOCHEMICAL COMPOSITION; BIOTRANSFORMATION; COLORIMETRY; ENZYME ACTIVITY; HYDROLYSIS; MORPHOLOGY; OPTIMIZATION; RACEMIZATION; REACTION KINETICS;

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BIOSYNTHESIS; DNA SEQUENCE; ENANTIOSELECTIVITY; ENZYME ACTIVITY; HYDROLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; RHODOCOCCUS ERYTHROPOLIS; TEMPERATURE MEASUREMENT; BIOTRANSFORMATION; CHEMISTRY; CLASSIFICATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; RHODOCOCCUS; STEREOISOMERISM;

BIOTRANSFORMATION; GLUTARATES; HYDROLYSIS; MOLECULAR SEQUENCE DATA; PHYLOGENY; RHODOCOCCUS; SOIL MICROBIOLOGY; STEREOISOMERISM;

RHODOCOCCUS ERYTHROPOLIS;

EID: 77955554529     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2584-5     Document Type: Article

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