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For recent examples, see, e.g.: (a) Q. B. Broxterman, B. Kaptein, J. Kamphuis, H. E. Schoemaker, J. Org. Chem. 1992, 57, 6286; (b) H. K. Chenault, J. Dahme, G. M. Whitesides, J. Am. Chem. Soc. 1989, 111, 6354; (c) R. J. Cox, W. A. Sherwin, L. K. P. Lam, J. C. Vederas, J. Am. Chem. Soc. 1996, 118, 7449; (d) E. J. Corey, M. C. Noe, F. Xu, Tetrahedron Lett. 1998, 39, 5347; (e) E. J. Corey, F. Xu, M. C. Noe, J. Am. Chem. Soc. 1997, 119, 12414; (f) M. J. O'Donnell, F. Delgado, C. Hostettler, R. Schwesinger, Tetrahedron Lett. 1998, 39, 8775; (g) M. J. Burk, J. G. Allen, W. F. Kiesman, J. Am. Chem. Soc. 1998, 120, 657.
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For recent examples, see, e.g.: (a) Q. B. Broxterman, B. Kaptein, J. Kamphuis, H. E. Schoemaker, J. Org. Chem. 1992, 57, 6286; (b) H. K. Chenault, J. Dahme, G. M. Whitesides, J. Am. Chem. Soc. 1989, 111, 6354; (c) R. J. Cox, W. A. Sherwin, L. K. P. Lam, J. C. Vederas, J. Am. Chem. Soc. 1996, 118, 7449; (d) E. J. Corey, M. C. Noe, F. Xu, Tetrahedron Lett. 1998, 39, 5347; (e) E. J. Corey, F. Xu, M. C. Noe, J. Am. Chem. Soc. 1997, 119, 12414; (f) M. J. O'Donnell, F. Delgado, C. Hostettler, R. Schwesinger, Tetrahedron Lett. 1998, 39, 8775; (g) M. J. Burk, J. G. Allen, W. F. Kiesman, J. Am. Chem. Soc. 1998, 120, 657.
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The two whole cell enzyme systems were permeabilized in different ways: the Pseudomonas putida strain was purchased from Novo Nordisk (Denmark) and permeabilized using proprietary knowledge; the GMO cells were produced and frozen at -80 °C for storage without cryprotection thus causing a suitable level of permeabilization
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