Identification and characterization of a novel nitrilase from Pseudomonas fluorescens Pf-5

Applied Microbiology and Biotechnology

Volumn 83, Issue 2, 2009, Pages 273-283

  Kim, Jung Soo ^a   Tiwari, Manish Kumar ^a   Moon, Hee Jung ^a   Jeya, Marimuthu ^a   Ramu, Thangadurai ^a   Oh, Deok Kun ^a   Kim, In Won ^a   Lee, Jung Kul ^b  

^a Konkuk University   (South Korea)

^b Konkuk University   (South Korea)

Author keywords

Aliphatic nitrilase; Cloning; Dinitrile; Pseudomonas fluorescens; Substrate specificity

Indexed keywords

ACTIVE SITES; ALIPHATIC NITRILASE; BIOCHEMICAL TOOLS; DINITRILE; DNA SEQUENCE ANALYSIS; ENZYMATIC SYNTHESIS; GEL-FILTRATION CHROMATOGRAPHIES; HOMOLOGY MODELING; ISO-ELECTRIC POINTS; NITRILE GROUPS; ONE-STEP REACTIONS; PSEUDOMONAS FLUORESCENS; SEQUENCE ANALYSIS; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SPECIFIC ACTIVITIES; SUBSTRATE SPECIFICITY; SUCCINONITRILE;

AMINES; AMINO ACIDS; CHROMATOGRAPHIC ANALYSIS; CLONING; CYANIDES; ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GELATION; GELS; INDUSTRIAL APPLICATIONS; NUCLEIC ACIDS; ORGANIC ACIDS; POLYMERS; SODIUM; SODIUM SULFATE;

SUBSTRATES;

AMINO ACID; DINITRILE; DNA; DODECYL SULFATE SODIUM; FUMARONITRILE; MONONITRILE; NITRILASE; NITRILE; POLYACRYLAMIDE GEL; PROTEIN; SUCCINONITRILE; UNCLASSIFIED DRUG;

AMINO ACID; AMMONIA; BACTERIUM; CARBOXYLIC ACID; CLONE; DNA; ENZYME; GENOME; HOMOLOGY; MOLECULAR ANALYSIS; PH; SUBSTRATE;

ARTICLE; BACTERIAL GENE; BIOINFORMATICS; DNA DETERMINATION; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE; GENE OVEREXPRESSION; GENOME; ISOELECTRIC POINT; MOLECULAR WEIGHT; NITPF5 GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PSEUDOMONAS FLUORESCENS; SEQUENCE ANALYSIS; SYNTHESIS;

AMINO ACID SEQUENCE; AMINOHYDROLASES; BACTERIAL PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PSEUDOMONAS FLUORESCENS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS FLUORESCENS PF-5;

EID: 67349190507     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-1862-6     Document Type: Article

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