The production, characterisation and enhanced pharmacokinetics of scFv-albumin fusions expressed in Saccharomyces cerevisiae

Protein Expression and Purification

Volumn 73, Issue 2, 2010, Pages 113-124

  Evans, Leslie ^a   Hughes, Miranda ^a   Waters, Joanne ^a   Cameron, Jason ^a   Dodsworth, Neil ^b   Tooth, David ^a   Greenfield, Anthony ^a   Sleep, Darrell ^a  

^a Novozymes Biopharma DK A S   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Albumin fusions; Heterologous protein expression; Saccharomyces cerevisiae; scFv; scFv albumin fusions

Indexed keywords

ALBUMINOID; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; HYBRID PROTEIN; SERUM ALBUMIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL; AREA UNDER THE CURVE; ARTICLE; BIOREACTOR; BIOSYNTHESIS; FERMENTATION; GENE FUSION; GENETICS; HALF LIFE TIME; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLIC CLEARANCE RATE; METABOLISM; RAT; SACCHAROMYCES CEREVISIAE; WISTAR RAT;

ALBUMINS; ANIMALS; AREA UNDER CURVE; ARTIFICIAL GENE FUSION; BIOREACTORS; FERMENTATION; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HALF-LIFE; HUMANS; METABOLIC CLEARANCE RATE; RATS; RATS, WISTAR; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SERUM ALBUMIN; SINGLE-CHAIN ANTIBODIES;

SACCHAROMYCES CEREVISIAE;

EID: 77955418625     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.05.009     Document Type: Article

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