Expressions of recombinant venom allergen, antigen 5 of yellowjacket (Vespula vulgaris) and paper wasp (Polistes annularis), in bacteria or yeast

Protein Expression and Purification

Volumn 16, Issue 3, 1999, Pages 410-416

  Monsalve, Rafael I ^a   Lu, Gang ^a   King, Te Piao ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN STRUCTURE; BACTERIUM; BIOLOGICAL FUNCTIONS; LEADER SEQUENCE; PARTIAL SEQUENCE; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

BACTERIA (MICROORGANISMS); PICHIA PASTORIS; POLISTES ANNULARIS; POLISTES FUSCATUS PALLIPES; VESPIDAE; VESPULA VULGARIS;

EID: 0033180105     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1082     Document Type: Article

References (22)

1. Lu G., Villalba M., Coscia M. R., Hoffman D. R., King T. P. Sequence analysis and antigen cross reactivity of a venom allergen antigen 5 from hornets, wasps and yellowjackets. J. Immunol. 150:1993;2823-2830.
2. King T. P., Lu G., Gonzalez M., Qian N. F., Soldatova L. Yellowjacket venom allergens, hyaluronidase and phosholipase: Sequence similarity and antigenic cross reactivity with their hornet and wasp homologs and possible implications for clinical allergy. J. Allergy Clin. Immunol. 98:1996;588-600.
3. Hoffman D. R. Allergens in Hymenoptera venom XXIV: The amino acid sequences of imported fire ant venom allergens Sol i II, Sol i III and Sol i IV. J. Allergy Clin. Immunol. 91:1995;71-78.
4. Hawdon J. M., Jones B. F., Hoffmann D. R., Hotez P. J. Cloning and characterization of ancylostoma-secreted protein. J. Biol. Chem. 271:1996;6672-6678.
5. Morrissette J., Kratzschmar J., Haendler B., El-Hayek R., Mochca-Morales J., Martin B. M., Patel J. R., Moss R. L., Schleuning W., Coronado R., Possani L. D. Primary structure and properties of helothermine, a peptide toxin that blocks ryanodine receptors. Biophys. J. 68:1995;2280-2288.
6. Mizuki N., Sarapata D. E., Garcia-Sanz J. A., Kasahara M. The mouse male germ cell-specific gene Tpx-1: Molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes. Mamm. Genome. 3:1992;274-280.
7. Murphy E. V., Zhang Y., Zhu W., Biggs J. The human glioma pathogenesis-related protein is structurally related to plant pathogenesis-related proteins and its gene is expressed specifically in brain tumors. Gene. 159:1995;131-135.
8. Cornelissen B. J. C., Hooft von Huijsduijnen R. A. M., Van Loon L. C., Bol J. F. Molecular characterization of messenger RNAs for pathogenesis related proteins 1a, 1b and 1c produced by TMV infection of tobacco. EMBO J. 5:1986;37-40.
9. King T. P., Kochoumian L., Lu G. Murine T and B cell responses to natural and recombinant hornet venom allergen, Dol m 5.02 and its recombinant fragments. J. Immunol. 154:1995;577-584.
10. Larsen J. N., Lowenstein H. Allergen nomenclature. J. Allergy Clin. Immunol. 97:1996;577-578.
11. Higgins D. R., Cregg J. M. Pichia Protocols. 1998;Humana Press, Totowa.
12. Linder S., Schliwa M., Kube-Granderath E. Direct PCR screening of Pichia pastoris clones. BioTechniques. 20:1996;980-982.
13. Graham I. R., Chambers A. Tuan R. S. Recombinant Gene Expression Protocols. 1997;159-169 Humana Press, Totowa.
14. Sambrook, J., Fritsch, E. F., and Maniatis, T.1989Molecular Cloning: A Laboratory Manual, pp. 9.31-9.58, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Singh R., Kats L. Catalysis of reduction of disulfide by selenol. Anal. Biochem. 232:1995;86-91.
16. Grassetti D. R., Murray J. F. Determination of sulfhydryl groups with 2,2′- or 4,4′-dithiodipyridine. Arch. Biochem. Biophy. 119:1967;41-49.
17. Cole P. A. Chaperon-assissted protein expression. Structure. 4:1996;239-242.
18. Bardwell J. C. Building bridges: Disulphide bond formation in the cell. Mol. Microbiol. 14:1994;199-205.
19. Okorokov A. L., Panov K. I., te Poele R. H., Breukelman H. J., Furia A., Karpeisky M. Y., Beintema J. J. An efficient system for active bovine pancreatic ribonuclease expression in E. coli. Protein Express. Purif. 6:1995;472-480.
20. Robbens J., Raemakers A., Steidler L., Fiers W., Remaut E. Production of soluble and active recombinant murine interlukin-2 in Escherichia coli: High level expressio, Kil-induced release and purification. Protein Express. Purif. 6:1995;481-486.
21. King T. P., Lu G., Agosto H. Antibody responses to bee melittin (Api m 4) and hornet antigen 5 (Dol m 5) in mice treated with the dominant T-cell epitope peptides. J. Allergy Clin. Immunol. 101:1998;397-403.
22. Tomalski M. D., King T. P., Miller L. K. Expression of hornet genes encoding venom allergen antigen 5 in insects. Arch. Insect Biochem. Physiol. 22:1993;303-313.