Correlation of levels of α-defensins determined by HPLC-esi-ms in bronchoalveolar lavage fluid with the diagnosis of pneumonia in premature neonates

Pediatric Research

Volumn 68, Issue 2, 2010, Pages 140-144

  Tirone, Chiara ^a   Boccacci, Simona ^a   Inzitari, Rosanna ^b   Tana, Milena ^a   Aurilia, Claudia ^a   Fanali, Chiara ^b   Cabras, Tiziana ^c   Messana, Irene ^c   Castagnola, Massimo ^b   Romagnoli, Costantino ^a   Vento, Giovanni ^a  

^a Department of Pediatrics ^*  (United States)

^b CATHOLIC UNIVERSITY   (Italy)

^c UNIVERSITY OF CAGLIARI   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA DEFENSIN;

ARTICLE; ARTIFICIAL VENTILATION; BIRTH WEIGHT; BLOODSTREAM INFECTION; CANDIDIASIS; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; ELECTROSPRAY MASS SPECTROMETRY; FEMALE; GESTATIONAL AGE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; LEUKOCYTE COUNT; LUNG LAVAGE; MALE; NEUTROPHIL COUNT; NEWBORN; PNEUMONIA; PREMATURITY; PRESCHOOL CHILD; PRIORITY JOURNAL; SCHOOL CHILD; STAPHYLOCOCCUS INFECTION; CHEMISTRY; CYTOLOGY; GENETICS; IMMUNOLOGY; INFANT, PREMATURE, DISEASES; INNATE IMMUNITY; MICROBIOLOGY; NEUTROPHIL; PREGNANCY;

ALPHA-DEFENSINS; BIRTH WEIGHT; BRONCHOALVEOLAR LAVAGE FLUID; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FEMALE; GESTATIONAL AGE; HUMANS; IMMUNITY, INNATE; INFANT, NEWBORN; INFANT, PREMATURE; INFANT, PREMATURE, DISEASES; MALE; NEUTROPHILS; PNEUMONIA; PREGNANCY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 77955152517     PISSN: 00313998     EISSN: 15300447     Source Type: Journal    
DOI: 10.1203/PDR.0b013e3181e5c242     Document Type: Article

References (28)

1. Bals R 2000 Epithelial antimicrobial peptides in host defense against infection. Respir Res 1:141-150
2. Yoshio H, Lagercrantz H, Gudmundsson GH, Agerberth B 2004 First line of defense in early human life. Semin Perinatol 28:304-311
3. Ganz T 1999 Defensins and Host defense. Science 286:420-421
4. Levy O 2000 Antimicrobial proteins and peptides of blood: templates for novel antimicrobial agents. Blood 96:2664-2672
5. Ganz T 2004 Antimicrobial polypeptides. J Leukoc Biol 75:34-38
6. Wilson CL, Ouellette AJ, Satchell DP, Ayabe T, López-Boado YS, Stratman JL, Hultgren SJ, Matrisian LM, Parks WC 1999 Regulation of Int.estinal a-defensin activation by metalloproteinase matrilysin in innate host defense. Science 286:113-117
7. Koczulla AR, Bals R 2003 Antimicrobial peptides: current status and therapeutic potential. Drugs 63:389-406
8. Cordero L, Ayers LW, Miller RR, Seguin JH, Coley BD 2002 Surveillance of ventilator-associated pneumonia in very-low-birth-weight infants. Am J Infect Control 30:32-39
9. Schaller-Bals S, Schulze A, Bals R 2002 Increased levels of antimicrobial peptides in tracheal aspirates of newborn infants during infection. Am J Respir Crit Care Med 165:992-995
10. Vento G, Romagnoli C, Zecca E, Matassa GP, Tortorolo L, De Carolis MP, d'Onofrio G, Zini G, Tommasi M, Fresu R, Zuppi C 1997 Increased levels of soluble Int.ercellular adhesion molecule-1,neutrophils and elastase in the lung of preterm infants with bronchopulmonary dysplasia. Prenat Neonatal Med 2:348-355
11. Vendettuoli V, De Cunto A, Tana M, Tirone C, Boccacci S, Vento G, Romagnoli C 2007 Pulmonary infections in preterm newborns. Minerva Pediatr 59:97-105
12. Vento G, Matassa PG, Ameglio F, Capoluongo E, Zecca E, Tortorolo L, Martelli M, Romagnoli C 2005 HFOV in premature neonates: effects on pulmonary mechanics and epithelial lining fluid cytokines. A randomized controlled trial. Int.ensive Care Med 31:463-470
13. Su BH, Watanabe T, Shimizu M, Yanagisawa M 1997 Echocardiographic assessment of patent ductus arteriosus shunt flow pattern in premature infants. Arch Dis Child Fetal Neonatal Ed 77:F36-F40
14. National Nosocomial Infection Survey Manual Available at: http://www.cdc.gov/ncidod/dhqp/pdf/nhsn/NHSNManual Patient Safety Protocol052407.pdf (Accessed November 16, 2009)
15. Vento G, Matassa PG, Zecca E, Tortorolo L, Martelli M, De Carolis MP, Maggio L, Zini G, D'Onofrio G, Valentini S, Romagnoli C 2004 Effect of dexamethasone on tracheobronchial aspirate fluid cytology and pulmonary mechanics in preterm infants. Pharmacology 71:113-119
16. Zhang Z, Marshall AG 1998 A universal algorithm for fast and automated charge state deconvolution of electrospray mass-to-charge ratio spectra. J Am Soc Mass Spectrom 9:225-233
17. Inzitari R, Vento G, Capoluongo E, Boccacci S, Fanali C, Cabras T, Romagnoli C, Giardina B, Messana I, Castagnola M 2007 Proteomic analysis of salivary acidic proline-rich proteins in human preterm and at-term newborns. J Proteome Res 6:1371-1377
18. Ong SE, Mann M 2005 Mass spectrometry-based proteomics turns quantitative. Nat Chem Biol 1:252-262
19. Vento G, Tirone C, Lulli P, Capoluongo E, Ameglio F, Lozzi S, Cota F, Mosca F, Romagnoli C, Messana I, Castagnola M, Inzitari R 2009 Bronchoalveolar lavage fluid peptidomics suggests a possible matrix metalloproteinase-3 role in bronchopulmonary dysplasia. Int.ensive Care Med 35:2115-2124
20. Dargaville PA, South M, Vervaart P, McDougall PN 1999 Validity of markers of dilution in small volume lung lavage. Am J Respir Crit Care Med 160:778-784
21. De Blic J, Midulla F, Barbato A, Clement A, Dab I, Eber E, Green C, Grigg J, Kotecha S, Kurland G, Pohunek P, Ratjen F, Rossi G 2000 Bronchoalveolar lavage in children. ERS Task Force on bronchoalveolar lavage in children. European Respiratory Society. Eur Respir J 15:217-231
22. Pisano E, Cabras T, Montaldo C, Piras V, Inzitari R, Olmi C, Castagnola M, Messana I 2005 Peptides of human gingival crevicular fluid determined by HPLC-ESI-MS. Eur J Oral Sci 113:462-468
23. Van Wetering S, Sterk PJ, Rabe KF, Hiemstra PS 1999 Defensins: key players or bystanders in infection, injury, and repair in the lung? J Allergy Clin Immunol 104:1131-1138
24. Huttner KM, Kozak CA, Bevins CL 1997 The mouse genome encodes a single homolog of the antimicrobial peptide human beta-defensin 1. FEBS Lett 413:45-49
25. Bals R, Goldman MJ, Wilson JM 1998 Mouse beta-defensin 1 is a salt sensitive antimicrobial peptide present in epithelia of the lung and urogenital tract. Infect Immun 66:1225-1232
26. Morrison GM, Davidson DJ, Kilanowski FM, Borthwick DW, Crook K, Maxwell AI, Govan JR, Dorin JR 1998 Mouse beta defensin-1 is a functional homolog of human/3-defensin-1. Mamm Genome 9:453-457
27. McMorran BJ, Patat SA, Carlin JB, Grimwood K, Jones A, Armstrong DS, Galati JC, Cooper PJ, Byrnes CA, Francis PW, Robertson CF, Hume DA, Borchers CH, Wainwright CE, Wainwright BJ 2007 Novel neutrophil-derived proteins in bronchoalveolar lavage fluid indicate an exaggerated inflammatory response in pediatric cystic fibrosis patients. Clin Chem 53:1782-1791
28. Vento G, Capoluongo E, Matassa PG, Concolino P, Vendettuoli V, Vaccarella C, Frezza S, Zuppi C, Romagnoli C, Ameglio F 2006 Serum levels of seven cytokines in premature ventilated newborns: correlations with old and new forms of bronchopulmonary dysplasia. Int.ensive Care Med 32:723-730