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Volumn 286, Issue 5437, 1999, Pages 113-117

Regulation of intestinal α-defensin activation by the metalloproteinase matrilysin in innate host defense

Author keywords

[No Author keywords available]

Indexed keywords

DEFENSIN; MATRILYSIN; METALLOPROTEINASE;

EID: 0033214433     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5437.113     Document Type: Article
Times cited : (944)

References (50)
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    • 2, 0.5% Tween-20, 1% bovine serum albumin (BSA), and 5% goat serum]. Sections were incubated overnight at 4°C with 1:5000 (antibody to matrilysin) or 1:1000 (antibody to cryptdin-1) dilutions of antibody in blocking solution. Sections were developed with the rabbit Vectastain Elite ABC kit (Vector Laboratories). Protein was visualized with True Blue substrate, and nuclei were counterstained with Contrast Red (Kirkegaard and Perry).
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    • We thank R. P. Mecham, T. J. Broekelmann, and A. H. Henschen for protein sequencing M. E. Selsted for the antibody to cryptdin-1 the cryptdin-4 peptide standard, and helpful discussions; M. A. Mulvey for help with bacteria and critical review of the manuscript; F. Heffron for the virulent 5. typhimurium strain 14028s; and S. Shapiro and J. Lohi for insightful comments on the manuscript. All animal experiments were performed with the approval of the Washington University School of Medicine Animal Studies Committee. This work was supported by a Barnes-Jewish Hospital Foundation grant (to C.L.W.) and by grants from NIH (to W.C.P., A.J.O., L.M.M., and S.J.H.).


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