Evaluation of ISO 6579 and FDA-BAM methods to complement real-time polymerase Chain reaction for the detection of salmonella in naturally contaminated poultry meat and red meat

Foodborne Pathogens and Disease

Volumn 7, Issue 8, 2010, Pages 921-927

  Eyigor, Aysegul ^a   Temelli, Seran ^a   Carli, Kamil Tayfun ^a  

^a ULUDAG UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; ARTICLE; BACTERIOLOGY; BACTERIUM DETECTION; CHICKEN MEAT; CONTAMINATION; FOOD AND DRUG ADMINISTRATION; MEDICAL SOCIETY; NONHUMAN; POULTRY; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RED MEAT; SALMONELLA; STANDARDIZATION; TURKEY (BIRD); UNITED STATES;

ANIMALS; ANIMALS, DOMESTIC; BACTERIAL TYPING TECHNIQUES; DNA, BACTERIAL; MEAT; POLYMERASE CHAIN REACTION; POULTRY; REPRODUCIBILITY OF RESULTS; SALMONELLA; SALMONELLA ENTERITIDIS; SALMONELLA FOOD POISONING; SALMONELLA TYPHIMURIUM; SEROLOGIC TESTS;

SALMONELLA;

EID: 77955079108     PISSN: 15353141     EISSN: None     Source Type: Journal    
DOI: 10.1089/fpd.2009.0497     Document Type: Article

References (33)

1. Anonymous. Certification report: AOAC Research Institute performance tested method SM 120301. Roche Diagnostics LightCycler Foodproof Salmonella spp. in combination with ShortPrep Foodproof 1 Kit. 2003a.
2. Anonymous. Regulation (EC) No. 2160/2003 of the European Parliament and of the Council of 17 November 2003 on the control of Salmonella and other specified food-borne zoonotic agents, European Union. Off J Eur Union L 2003b;325:1-15.
3. Anonymous. Product information of LightCycler Foodproof Salmonella Detection Kit Insert, Roche Diagnostics. Version 2006. Available at . (Online.)
4. Badosa E, Chico N, Pla M, Pares D, and Montesinos E. Evaluation of ISO enrichment real-time PCR methods with internal amplification control for detection of Listeria monocytogenes and Salmonella enterica in fresh fruit and vegetables. Lett Appl Microbiol 2009;49:105-111.
5. Baumler AJ, Hargis BM, and Tsolis RM. Tracing the origins of Salmonella outbreaks. Science 2000;287:50-52.
6. Bohaychuk VM, Gensler GE, Mcfall ME, King RK, and Renter DG. A real time PCR assay for the detection of Salmonella in a wide variety of food and food-animal matrices. J Food Prot 2007;70:1080-1087.
7. Catarame TMG. Comparison of a real-time polymerase chain reaction assay with a culture method for the detection of Salmonella in retail meat samples. J Food Saf 2006;26:11-15.
8. Cheung PY, Chan CW, Wong W, Cheung TL, and Kam KM. Evaluation of two real-time polymerase chain reaction pathogen detection kits for Salmonella spp. in food. Lett Appl Mi- crobiol 2004;39:509-515.
9. Croci L, Delibato E, Volpe G, De Medici D, and Palleschi G. Comparison of PCR, electrochemical enzyme-linked immu- nosorbent assays, and the standard culture method for detecting Salmonella in meat products. Appl Environ Microbiol 2004;70:1393-1396.
10. [DEFRA] Department for Environment, Food and Rural Affairs. Code of Practice for the Prevention and Control of Salmonella in Chickens Reared for Meat on Farm. London: DEFRA, 2002.
11. Ellingson JLE, Anderson JL, Carlson AS, and Sharma VK. Twelve hour real-time PCR technique for the sensitive and specific detection of Salmonella in raw and ready-to-eat meat products. Mol Cell Probes 2004;18:51-57.
12. Eyigor A and Carli KT. Rapid detection of Salmonella from poultry by real-time polymerase chain reaction with fluorescent hybridization probes. Avian Dis 2003;47:380-386.
13. Eyigor A, Carli KT, and Unal CB. Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. Lett Appl Microbiol 2002;34:37-41.
14. Fakhr MK, McEvoy JM, Sherwood JS, and Logue CM. Adding a selective enrichment step to the iQ-CheckTM real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products. Lett Appl Microbiol 2006;43:78-83.
15. [FDA] Food and Drug Administration. Salmonella. In: Bacteriological Analytical Manual, Chapter 5. Andrews WH and Hammack T (eds.). Available at Research/LaboratoryMethods/BacteriologicalAnalyticalManual BAM/ucm070149.htm, accessed December, 2007. (Online.)
16. Hargis BM, Caldwell DJ, Brewer RL, Corrier DE, and Deloach JR. Evaluation of the chicken crop as a source of Salmonella contamination for broiler carcasses. Poult Sci 1995;74:1548-1552.
17. Hein I, Flekna G, Krassnig M, and Wagner M. Real-time PCR for the detection of Salmonella spp. in food: an alternative approach to a conventional PCR system suggested by the FOOD-PCR Project. J Microbiol Methods 2006;66:538-547.
18. Hoorfar J, Cook N, Malorny B, Wagner M, De Medici D, Ab- dulmawjood A, and Fach P. Making internal amplification control mandatory for diagnostic PCR. J Clin Microbiol 2003; 41:5835.
19. [ISO] International Organization for Standardization. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Detection of Salmonella spp. ISO 6579:2002(E). Geneva, Switzerland: International Organization for Standardization, 2002.
20. [ISO] International Organization for Standardization. Microbiology of Food and Animal Feeding Stuffs-Protocol for the Validation of Alternative Methods. ISO 16140:2003(E). Geneva, Switzerland: International Organization for Standardization, 2003.
21. Jothikumar N, Wang X, and Griffiths MW. Real-time multiplex SYBR Green I-based PCR assay for simultaneous detection of Salmonella serovars and Listeria monocytogenes. J Food Prot 2003;66:2141-2145.
22. Krascsenicsova K, Piknova L, Kaclikova E, and Kuchta T. Detection of Salmonella enterica in food using two-step enrichment and polymerase chain reaction. Lett Appl Microbiol 2008;46:483-487.
23. Leon-Velarde CG, Cai HY, Larkin C, Bell-Rogers P, Stevens RWC, and Odumeru JA. Evaluation of methods for the identification of Salmonella enterica serotype Typhimurium DT104 from poultry environmental samples. J Microbiol Methods 2004;58:79-86.
24. Lofstrom C, Knutsson R, Axelsson CE, and Radstrom P. Rapid and specific detection of Salmonella spp. in animal feed samples by PCR after culture enrichment. Appl Environ Microbiol 2004;70:69-75.
25. Malorny B, Bunge C, and Helmuth R. A real-time PCR for the detection of Salmonella Enteritidis in poultry meat and consumption eggs. J Microbiol Methods 2007;70:245-251.
26. Mercanoglu B and Griffiths MW. Combination of immuno- magnetic separation with real-time PCR for rapid detection of Salmonella in milk, ground beef, and alfalfa sprouts. J Food Prot 2005;68:557-561.
27. Olsen SJ, MacKinnon LC, Goulding JS, Bean NH, and Slutsker L. Surveillance for foodborne-disease outbreaks-United States, 1993-1997. Morb Mortal Wkly Rep 2000;49:1-62.
28. O'Regan E, Mccabe E, Burgess C, Mcguinness S, Barry T, Duffy G, Whyte P, and Fanning S. Development of a realtime multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. BMC Microbiol 2008;156: 1-11.
29. Perelle S, Dilasser F, Malorny B, Graut J, Hoorfar J, and Fach P. Comparison of PCR-ELISA and LightCycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples. Mol Cell Probes 2004;18:409-420.
30. Schrank IS, Mores MA, Costa JL, Frazzon AP, Soncini R, Schrank A, Vainstein MH, and Silva SC. Influence of enrichment media and application of a PCR based method to detect Salmonella in poultry industry products and clinical samples. Vet Microbiol 2001;82:45-53.
31. [TFC] Turkish Food Codex. Directive on Microbiological Criteria. Directive Number: 2009/6, Ankara, 2009.
32. Uyttendaele M, Vanwildemeersch K, and Debevere J. Evaluation of real-time PCR vs automated ELISA and a conventional culture method using a semi-solid medium for detection of Salmonella. Lett Appl Microbiol 2003;37:386-391.
33. Wang X, Jothikumar N, and Griffiths MW. Enrichment and DNA extraction protocols for the simultaneous detection of Salmonella and Listeria monocytogenes in raw sausage meat with multiplex real-time PCR. J Food Prot 2004;67:189-192.