shRNA expression constructs designed directly from siRNA oligonucleotide sequences

Molecular Biotechnology

Volumn 45, Issue 2, 2010, Pages 116-120

  Barøy, Tuva ^a,b   Sørensen, Kirsten ^a   Lindeberg, Mona Mari ^b   Frengen, Eirik ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

Cloning; shRNA; shRNA cassette; siRNA; Transfection

Indexed keywords

CLONING; GENETIC ENGINEERING; MAMMALS; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; RNA;

OLIGONUCLEOTIDE SEQUENCES; QUANTITATIVE REAL TIME PCR; SHORT HAIRPIN RNA (SHRNA); SHRNA; SHRNA CASSETTE; SIRNA; SMALL INTERFERING RNA (SIRNA); TRANSFECTION;

GENE EXPRESSION;

COMPLEMENTARY DNA; MESSENGER RNA; OLIGONUCLEOTIDE; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; SPACER DNA;

ARTICLE; CELL CULTURE; CELL STRAIN HEK293; CONTROLLED STUDY; GENE CONSTRUCT; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

MAMMALIA;

EID: 77954957161     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9247-8     Document Type: Article

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