Analysis of ionizing radiation-induced DNA damage and repair in three-dimensional human skin model system

Experimental Dermatology

Volumn 19, Issue 8, 2010, Pages

  Su, Yanrong ^a   Meador, Jarah A ^a   Geard, Charles R ^a   Balajee, Adayabalam S ^a  

^a NewYork Presbyterian Hospital   (United States)

Author keywords

53 binding protein 1; Double strand break induction and repair; EpiDerm tissue constructs; Low LET radiation; Phosphatidylinositol 3 kinase like kinases

Indexed keywords

53 BINDING PROTEIN 1; ATM PROTEIN; BINDING PROTEIN; BIOLOGICAL MARKER; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; ARTIFICIAL SKIN; CONTROLLED STUDY; DNA REPAIR; DNA STRAND BREAKAGE; GAMMA RADIATION; HUMAN; HUMAN CELL; RADIATION DOSE; RADIATION INJURY; RADIATION RESPONSE;

BIOLOGICAL MARKERS; CELL LINE; CHROMONES; DNA BREAKS, DOUBLE-STRANDED; DNA DAMAGE; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, RADIATION; ENZYME INHIBITORS; FIBROBLASTS; GAMMA RAYS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MODELS, BIOLOGICAL; MORPHOLINES; PHOSPHATIDYLINOSITOL 3-KINASES; RADIATION, IONIZING; SIGNAL TRANSDUCTION; SKIN;

EID: 77954845113     PISSN: 09066705     EISSN: 16000625     Source Type: Journal    
DOI: 10.1111/j.1600-0625.2009.00945.x     Document Type: Article

References (34)

1. Durocher D, Jackson S P. DNA-PK, ATM and ATR as sensors of DNA damage: variations on a theme? Curr Opin Cell Biol 2001: 13: 225-231.
2. Kinner A, Wu W, Staudt C, Iliakis G. Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res 2008: 36: 5678-5694.
3. Kobayashi J, Iwabuchi K, Miyagawa K, et al. Current topics in DNA doublestrand break repair. J Radiat Res (Tokyo) 2008: 49: 93-103.
4. Riches L C, Lynch A M, Gooderham N J. Early events in the mammalian response to DNA double-strand breaks. Mutagenesis 2008: 23: 331-339.
5. Shiloh Y. ATM and related protein kinases: safeguarding genome integrity. Nat Rev Cancer 2003: 3: 155-168.
6. Bakkenist C J, Kastan M B. DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature 2003: 421: 499-506.
7. Stiff T, O'Driscoll M, Rief N, Iwabuchi K, Lobrich M, Jeggo P A. ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation. Cancer Res 2004: 64: 2390-2396.
8. Balajee A S, Geard C R. Replication protein A and gamma-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks. Exp Cell Res 2004: 300: 320-334.
9. Bassing C H, Alt F W. H2AX may function as an anchor to hold broken chromosomal DNA ends in close proximity. Cell Cycle 2004: 3: 149-153.
10. Bassing C H, Alt F W. The cellular response to general and programmed DNA double strand breaks. DNA Repair (Amst) 2004: 3: 781-796.
11. Jackson S P. Sensing and repairing DNA double-strand breaks. Carcinogenesis 2002: 23: 687-696.
12. Thacker J, Zdzienicka M Z. The XRCC genes: expanding roles in DNA doublestrand break repair. DNA Repair (Amst) 2004: 3: 1081-1090.
13. Thompson L H, Schild D. Recombinational DNA repair and human disease. Mutat Res 2002: 509: 49-78.
14. Balajee A S, Geard C R. Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acids Res 2001: 29: 1341-1351.
15. Li H, Balajee A S, Su T, Cen B, Hei T K, Weinstein I B. The HINT1 tumor suppressor regulates both gamma-H2AX and ATM in response to DNA damage. J Cell Biol 2008: 183: 253-265.
16. Meador J A, Zhao M, Su Y, Narayan G, Geard C R, Balajee A S. Histone H2AX is a critical factor for cellular protection against DNA alkylating agents. Oncogene 2008: 27: 5662-5671.
17. Stenerlow B, Karlsson K H, Cooper B, Rydberg B. Measurement of prompt DNA double-strand breaks in mammalian cells without including heat-labile sites: results for cells deficient in nonhomologous end joining. Radiat Res 2003: 159: 502-510.
18. Cedervall B, Radivoyevitch T. Methods for analysis of DNA fragment distributions on pulsed field gel electrophoretic gels. Electrophoresis 1996: 17: 1080- 1086.
19. Ruiz de Almodovar J M, Steel G G, Whitaker S J, McMillan T J. A comparison of methods for calculating DNA double-strand break induction frequency in mammalian cells by pulsed-field gel electrophoresis. Int J Radiat Biol 1994: 65: 641-649.
20. Rogakou E P, Boon C, Redon C, Bonner W M. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol 1999: 146: 905-916.
21. Rogakou E P, Pilch D R, Orr A H, Ivanova V S, Bonner W M. DNA doublestranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998: 273: 5858-5868.
22. Anderson L, Henderson C, Adachi Y. Phosphorylation and rapid relocalization of 53BP1 to nuclear foci upon DNA damage. Mol Cell Biol 2001: 21: 1719- 1729.
23. Goldberg M, Stucki M, Falck J, et al. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature 2003: 421: 952-956.
24. Rappold I, Iwabuchi K, Date T, Chen J. Tumor suppressor p53 binding protein (53BP1) is involved in DNA damage-signaling pathways. J Cell Biol 2001: 153: 613-620.
25. Schultz L B, Chehab N H, Malikzay A, Halazonetis T D. p53 binding protein 1 (53BP1) is an early participant in the cellular response to DNA double-strand breaks. J Cell Biol 2000: 151: 1381-1390.
26. Stewart G S, Wang B, Bignell C R, Taylor A M, Elledge S J. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature 2003: 421: 961-966.
27. Adams M M, Carpenter P B. Tying the loose ends together in DNA double strand break repair with 53BP1. Cell Div 2006: 1: 19.
28. DiTullio R A Jr, Mochan T A, Venere M, et al. 53BP1 functions in an ATMdependent checkpoint pathway that is constitutively activated in human cancer. Nat Cell Biol 2002: 4: 998-1002.
29. Fernandez-Capetillo O, Chen H T, Celeste A, et al. DNA damage-induced G2-M checkpoint activation by histone H2AX and 53BP1. Nat Cell Biol 2002: 4: 993-997.
30. Ward I M, Minn K, Jorda K G, Chen J. Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX. J Biol Chem 2003: 278: 19579-19582.
31. Ward I M, Minn K, van Deursen J, Chen J. p53 Binding protein 53BP1 is required for DNA damage responses and tumor suppression in mice. Mol Cell Biol 2003: 23: 2556-2563.
32. Costes S V, Ponomarev A, Chen J L, Nguyen D, Cucinotta F A, Barcellos-Hoff M H. Image-based modeling reveals dynamic redistribution of DNA damage into nuclear sub-domains. PLoS Comput Biol 2007: 3: e155.
33. Lee Y S, Sohn K C, Kim K H, et al. Role of protein kinase C delta in X-rayinduced apoptosis of keratinocyte. Exp Dermatol 2009: 18: 50-56.
34. Lee H, Kwak H J, Cho I T, Park S H, Lee C H. S1219 residue of 53BP1 is phosphorylated by ATM kinase upon DNA damage and required for proper execution of DNA damage response. Biochem Biophys Res Commun 2009: 378: 32-36.