Evolved Lactococcus lactis Strains for Enhanced Expression of Recombinant Membrane Proteins

Journal of Molecular Biology

Volumn 401, Issue 1, 2010, Pages 45-55

  Linares, Daniel M ^a   Geertsma, Eric R ^a   Poolman, Bert ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Directed evolution; Evolved expression hosts; Folding indicator; GFP; Membrane proteins

Indexed keywords

ERYTHROMYCIN; GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; GENE MUTATION; LACTOCOCCUS LACTIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN TARGETING; SEQUENCE ANALYSIS;

LACTOCOCCUS LACTIS;

EID: 77954760688     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.06.002     Document Type: Article

References (36)

1. Grisshammer R. Understanding recombinant expression of membrane proteins. Curr. Opin. Biotechnol. 2006, 17:337-340.
2. Widmann M., Christen P. Comparison of folding rates of homologous prokaryotic and eukaryotic proteins. J. Biol. Chem. 2000, 275:18619-18622.
3. Zhang G., Hubalewska M., Ignatova Z. Transient ribosomal attenuation coordinates protein synthesis and co-translational folding. Nat. Struct. Mol. Biol. 2009, 16:274-280.
4. Wagner S., Baars L., Ytterberg A.J., Klussmeier A., Wagner C.S., Nord O., et al. Consequences of membrane protein overexpression in Escherichia coli. Mol. Cell. Proteomics 2007, 9:1527-1550.
5. Georgiou G., Valax P. Expression of correctly folded proteins in Escherichia coli. Curr. Opin. Biotechnol. 1996, 7:190-197.
6. Kunji E.R., Slotboom D.J., Poolman B. Lactococcus lactis as host for overproduction of functional membrane proteins. Biochim. Biophys. Acta 2003, 1610:97-108.
7. Quick M., Javitch J.A. Monitoring the function of membrane transport proteins in detergent-solubilized form. Proc. Natl Acad. Sci. USA 2007, 104:3603-3608.
8. Morello E., Bermúdez-Humarán L.G., Llull D., Solé V., Miraglio N., Langella P., et al. Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion. J. Mol. Microbiol. Biotechnol. 2008, 14:48-58.
9. Frelet-Barrand A., Boutigny S., Kunji E.R., Rolland N. Membrane protein expression in Lactococcus lactis. Methods Mol. Biol. 2010, 601:67-85.
10. Mulligan C., Geertsma E.R., Severi E., Kelly D.J., Poolman B., Thomas G.H. The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter. Proc. Natl Acad. Sci. USA 2009, 106:1778-1783.
11. Kunji E.R., Chan K.W., Slotboom D.J., Floyd S., O'Connor R., Monné M. Eukaryotic membrane protein overproduction in Lactococcus lactis. Curr. Opin. Biotechnol. 2005, 16:546-551.
12. Janvilisri T., Shahi S., Venter H., Balakrishnan L., van Veen H.W. Arginine-482 is not essential for transport of antibiotics, primary bile acids and unconjugated sterols by the human breast cancer resistance protein (ABCG2). Biochem. J. 2005, 385:419-426.
13. Serrano-Vega M.J., Magnani F., Shibata Y., Tate C.G. Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form. Proc. Natl Acad. Sci. USA 2008, 105:877-882.
14. Magnani F., Shibata Y., Serrano-Vega M.J., Tate C.G. Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor. Proc. Natl Acad. Sci. USA 2008, 105:10744-10749.
15. Warne T., Serrano-Vega M.J., Baker J.G., Moukhametzianov R., Edwards P.C., Henderson R., et al. Structure of a beta1-adrenergic G-protein-coupled receptor. Nature 2008, 454:486-491.
16. Shibata Y., White J.F., Serrano-Vega M.J., Magnani F., Aloia A.L., Grisshammer R., et al. Thermostabilization of the neurotensin receptor NTS1. J. Mol. Biol. 2009, 390:262-277.
17. Waldo G.S., Standish B.M., Berendzen J., Terwilliger T.C. Rapid protein-folding assay using green fluorescent protein. Nat. Biotechnol. 1999, 17:691-695.
18. Drew D., Lerch M., Kunji E., Slotboom D.J., de Gier J.W. Optimization of membrane protein overexpression and purification using GFP fusions. Nat. Methods 2006, 3:303-313.
19. Geertsma E.R., Groeneveld M., Slotboom D.J., Poolman B. Quality control of integral membrane proteins. Proc. Natl Acad. Sci. USA 2008, 105:5722-5727.
20. Niu Y., Kong J., Xu Y. A novel GFP-fused eukaryotic membrane protein expression system in Lactococcus lactis and its application to overexpression of an elongase. Curr. Microbiol. 2008, 57:423-428.
21. MacLean D., Jones J.D., Studholme D.J. Application of next-generation sequencing technologies to microbial genetics. Nat. Rev., Microbiol. 2009, 7:287-296.
22. Srivatsan A., Han Y., Peng J., Tehranchi A.K., Gibbs R., Wang J.D., Chen R. High-precision, whole-genome sequencing of laboratory strains facilitates genetic studies. PLoS Genet. 2008, 4:e1000139.
23. Waldo G.S. Improving protein folding efficiency by directed evolution using the GFP folding reporter. Methods Mol. Biol. 2003, 230:343-359.
24. Skretas G., Georgiou G. Genetic analysis of G protein-coupled receptor expression in Escherichia coli: inhibitory role of DnaJ on the membrane integration of the human central cannabinoid receptor. Biotechnol. Bioeng. 2009, 102:357-367.
25. Wagner S., Bader M.L., Drew D., de Gier J.W. Rationalizing membrane protein overexpression. Trends Biotechnol. 2006, 8:364-371.
26. Villa R., Lotti M., Gatti-Lafranconi P. Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm. Microb. Cell Fact. 2009, 8:32-42.
27. Kolaj O., Spada S., Robin S., Wall J.G. Use of folding modulators to improve heterologous protein production in Escherichia coli. Microb. Cell Fact. 2009, 8:9-25.
28. Marreddy.R., Geertsma E.R., Permentier H.P., Pinto J., Kok J., Poolman B. Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis. PLoS ONE 2010, 5:e10317.
29. Miroux B., Walker J.E. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 1996, 260:289-298.
30. Wagner S., Klepsch M.M., Schlegel S., Appel A., Draheim R., Tarry M., et al. Tuning Escherichia coli for membrane protein overexpression. Proc. Natl Acad. Sci. USA 2008, 105:14371-14376.
31. Massey-Gendel E., Zhao A., Boulting G., Kim H.Y., Balamotis M.A., Seligman L.M., et al. Genetic selection system for improving recombinant membrane protein expression in E. coli. Protein Sci. 2009, 18:372-383.
32. Mascher T., Helmann J.D., Unden G. Stimulus perception in bacterial signal-transducing histidine kinases. Microbiol. Mol. Biol. Rev. 2006, 70:910-938.
33. Leenhouts K., Buist G., Bolhuis A., ten Berge A., Kiel J., Mierau I., et al. A general system for generating unlabelled gene replacements in bacterial chromosomes. Mol. Gen. Genet. 1996, 253:217-224.
34. Geertsma E.R., Poolman B. High-throughput cloning and expression in recalcitrant bacteria. Nat. Methods 2007, 4:705-707.
35. Casadaban M.J., Cohen S.N. Analysis of gene control signals by DNA fusion in Escherichia coli. J. Mol. Biol. 1980, 138:179-207.
36. Maguin E., Duwat P., Hege T., Ehrlich D., Gruss A. New thermosensitive plasmid for gram-positive bacteria. J. Bacteriol. 1992, 174:5633-5638.