Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis

PLoS ONE

Volumn 5, Issue 4, 2010, Pages

  Marreddy, Ravi K R ^a   Geertsma, Eric R ^a,b   Permentier, Hjalmar P ^a   Pinto, Joao P C ^a   Kok, Jan ^a   Poolman, Bert ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID TRANSPORTER; BRANCHED CHAIN AMINO ACID; DIPEPTIDE; ISOLEUCINE; LEUCINE; MEMBRANE PROTEIN; VALINE; AMINO ACID; BACTERIAL PROTEIN; PROTEIN; PROTEOME;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; LACTOBACILLUS DELBRUECKII; NONHUMAN; NUTRIENT; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PROTEOMICS; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENETICS; LACTOCOCCUS LACTIS; METABOLISM;

AMINO ACID TRANSPORT SYSTEMS; AMINO ACIDS; AMINO ACIDS, BRANCHED-CHAIN; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; LACTOCOCCUS LACTIS; PROTEIN BIOSYNTHESIS; PROTEINS; PROTEOME;

EID: 77954757970     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0010317     Document Type: Article

References (39)

1. Grisshammer R (2006) Understanding recombinant expression of membrane proteins. Curr Opin Biotechnol 17: 337-340.
2. Geertsma ER, Poolman B (2007) High-throughput cloning and expression in recalcitrant bacteria. Nat Methods 4: 705-707.
3. Mierau I, Kleerebezem M (2005) 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl Microbiol Biotechnol 68: 705-717.
4. Morello E, Bermudez-Humaran LG, Llull D, Sole V, Miraglio N, et al. (2008) Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion. J Mol Microbiol Biotechnol 14: 48-58.
5. Kunji ER, Slotboom DJ, Poolman B (2003) Lactococcus lactis as host for overproduction of functional membrane proteins. Biochim Biophys Acta 1610: 97-108.
6. Mulligan C, Geertsma ER, Severi E, Kelly DJ, Poolman B, et al. (2009) The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter. Proc Natl Acad Sci U S A 106: 1778-1783.
7. Quick M, Javitch JA (2007) Monitoring the function of membrane transport proteins in detergent-solubilized form. Proc Natl Acad Sci U S A 104: 3603-3608.
8. Berntsson RP, Doeven MK, Fusetti F, Duurkens RH, Sengupta D, et al. (2009) The structural basis for peptide selection by the transport receptor OppA. EMBO J 28: 1332-1340.
9. de Ruyter PG, Kuipers OP, de Vos WM (1996) Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl Environ Microbiol 62: 3662-3667.
10. El KM, van Roosmalen ML, Jager D, Metselaar H, Permentier H, et al. (2008) Lactococcus lactis as expression host for the biosynthetic incorporation of tryptophan analogues into recombinant proteins. Biochem J 409: 193-198.
11. Berntsson RP, Alia ON, Fusetti F, Thunnissen AM, Poolman B, et al. (2009) Selenomethionine incorporation in proteins expressed in Lactococcus lactis. Protein Sci 18: 1121-1127.
12. Gorgens JF, van Zyl WH, Knoetze JH, Hahn-Hagerdal B (2005) Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium. Appl Microbiol Biotechnol 67: 684-691.
13. Toman PD, Chisholm G, McMullin H, Giere LM, Olsen DR, et al. (2000) Production of recombinant human type I procollagen trimers using a four-gene expression system in the yeast Saccharomyces cerevisiae. J Biol Chem 275: 23303-23309.
14. Poolman B, Konings WN (1988) Relation of growth of Streptococcus lactis and Streptococcus cremoris to amino acid transport. J Bacteriol 170: 700-707.
15. Waldo GS, Standish BM, Berendzen J, Terwilliger TC (1999) Rapid protein-folding assay using green fluorescent protein. Nat Biotechnol 17: 691-695.
16. Drew DE, Von HG, Nordlund P, De Gier JW (2001) Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett 507: 220-224.
17. den Hengst CD, van Hijum SA, Geurts JM, Geurts JM, Nauta A, et al. (2005) The Lactococcus lactis CodY regulon: identification of a conserved cis-regulatory element. J Biol Chem 280: 34332-34342.
18. Kuipers OP, de Ruyter PGGA, Kleerebezem M, de Vos WM (1998) Quorum sensing-controlled gene expression in lactic acid bacteria. Journal of Biotechnology 64: 15-21.
19. Guedon E, Serror P, Ehrlich SD, Renault P, Delorme C (2001) Pleiotropic transcriptional repressor CodY senses the intracellular pool of branched-chain amino acids in Lactococcus lactis. Mol Microbiol 40: 1227-1239.
20. Kunji ER, Smid EJ, Plapp R, Poolman B, Konings WN (1993) Di-tripeptides and oligopeptides are taken up via distinct transport mechanisms in Lactococcus lactis. J Bacteriol 175: 2052-2059.
21. Kunji ER, Hagting A, De Vries CJ, Juillard V, Haandrikman AJ, et al. (1995) Transport of beta-casein-derived peptides by the oligopeptide transport system is a crucial step in the proteolytic pathway of Lactococcus lactis. J Biol Chem 270: 1569-1574.
22. den Hengst CD, Groeneveld M, Kuipers OP, Kok J (2006) Identification and functional characterization of the Lactococcus lactis CodY-regulated branched-chain amino acid permease BcaP (CtrA). J Bacteriol 188: 3280-3289.
23. Geertsma ER, Groeneveld M, Slotboom DJ, Poolman B (2008) Quality control of overexpressed membrane proteins. Proc Natl Acad Sci U S A 105: 5722-5727.
24. Ngo JT, Champion JA, Mahdavi A, Tanrikulu IC, Beatty KE, et al. (2009) Cell-selective metabolic labeling of proteins. Nat Chem Biol 5: 715-717.
25. Terzaghi BE, Sandine WE (1975) Improved Medium for Lactic Streptococci and Their Bacteriophages. Appl Microbiol 29: 807-813.
26. Driessen AJ, de Jong S, Konings WN (1987) Transport of branched-chain amino acids in membrane vesicles of Streptococcus cremoris. J Bacteriol 169: 5193-5200.
27. Linares DM, Geertsma ER, Poolman B (2010) Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins. Under review.
28. Hagting A, Knol J, Hasemeier B, Streutker MR, Fang G, et al. (1997) Amplified expression, purification and functional reconstitution of the dipeptide and tripeptide transport protein of Lactococcus lactis. Eur J Biochem 247: 581-587.
29. Detmers FJ, Lanfermeijer FC, Abele R, Jack RW, Tampe R, et al. (2000) Combinatorial peptide libraries reveal the ligand-binding mechanism of the oligopeptide receptor OppA of Lactococcus lactis. Proc Natl Acad Sci U S A 97: 12487-12492.
30. Simon D, Chopin A (1988) Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. Biochimie 70: 559-566.
31. Solem C, Defoor E, Jensen PR, Martinussen J (2008) Plasmid pCS1966, a new selection/counterselection tool for lactic acid bacterium strain construction based on the oroP gene, encoding an orotate transporter from Lactococcus lactis. Appl Environ Microbiol 74: 4772-4775.
32. Jensen PR, Hammer K (1993) Minimal Requirements for Exponential Growth of Lactococcus lactis. Appl Environ Microbiol 59: 4363-4366.
33. Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, et al. (2000) The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21: 1037-1053.
34. Wegmann U, O'Connell-Motherway M, Zomer A, Buist G, Shearman C, et al. (2007) Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp. cremoris MG1363. J Bacteriol 189: 3256-3270.
35. Poolman B, Smid EJ, Veldkamp H, Konings WN (1987) Bioenergetic consequences of lactose starvation for continuously cultured Streptococcus cremoris. J Bacteriol 169: 1460-1468.
36. Brooijmans RJ, Poolman B, Schuurman-Wolters GK, de Vos WM, Hugenholtz J (2007) Generation of a membrane potential by Lactococcus lactis through aerobic electron transport. J Bacteriol 189: 5203-5209.
37. van der Heide T, Poolman B (2000) Osmoregulated ABC-transport system of Lactococcus lactis senses water stress via changes in the physical state of the membrane. Proc Natl Acad Sci U S A 97: 7102-7106.
38. Biemans-Oldehinkel E, Poolman B (2003) On the role of the two extra-cytoplasmic substrate-binding domains in the ABC transporter OpuA. EMBO J 22: 5983-5993.
39. Duurkens RH, Tol MB, Geertsma ER, Permentier HP, Slotboom DJ (2007) Flavin binding to the high affinity riboflavin transporter RibU. J Biol Chem 282: 10380-10386.