A real-time PCR diagnostic method for detection of Naegleria fowleri

Experimental Parasitology

Volumn 126, Issue 1, 2010, Pages 37-41

  Maďarová, Lucia ^a   Trnková, Katarína ^a   Feiková, Soňa ^a   Klement, Cyril ^a   Obernauerová, Margita ^b  

^a Regional Authority of Public Health   (Slovakia)

^b Faculty of Natural Sciences   (Slovakia)

Author keywords

Diagnosis; Hybridization probes; Naegleria fowleri; Real time PCR

Indexed keywords

PROTEIN MP2CL5; PROTOZOAL PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CEREBROSPINAL FLUID CULTURE; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC ACCURACY; FLUORESCENCE ANALYSIS; GENE SEQUENCE; HEALTH HAZARD; MICROORGANISM DETECTION; NAEGLERIA FOWLERI; NONHUMAN; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SPECIES DIFFERENCE; SWIMMING POOL; WATER CONTAMINATION;

CENTRAL NERVOUS SYSTEM PROTOZOAL INFECTIONS; COMPUTER SYSTEMS; DNA PRIMERS; DNA, PROTOZOAN; FLUORESCENT DYES; FRESH WATER; LIMIT OF DETECTION; MEMBRANE PROTEINS; NAEGLERIA FOWLERI; POLYMERASE CHAIN REACTION; PROTOZOAN PROTEINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SOFTWARE; SWIMMING POOLS; TIME FACTORS;

AMOEBA (GENUS); EUKARYOTA; NAEGLERIA FOWLERI; PROKARYOTA;

EID: 77954757829     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2009.11.001     Document Type: Article

References (32)

1. Behets J., Declerck P., Delaedt Y., Verelst L., Ollevier F. A duplex real-time PCR assay for quantitative detection of Naegleria fowleri in water samples. Water Research 2007, 41:118-126.
2. Behets J., Declerck P., Delaedt Y., Verelst L., Ollevier F. Quantitative detection and differentiation of free-living amoeba species using SYBR green-based real-time PCR melting curve analysis. Current Microbiology 2006, 53:506-509.
3. Behets J., Seghi F., Declerck P., Verelst L., Duvivier L., Van Damme A., Ollevier F. Detection of Naegleria spp. and Naegleria fowleri: comparison of flagellaltion tests, ELISA and PCR. Water Science Technology 2003, 47:117-122.
4. De Jonckheere J.F., Brown S., Dobson P.J., Robinson B.S., Pernin P. The amoeba-to-flagellate transformation test is not reliable for the diagnosis of the genus Naegleria. Description of three new Naegleria spp. Protist 2001, 152:115-121.
5. De Jonckheere J.F. Characterisation of Naegleria by restriction endonuclease digestion of whole-cell DNA. Molecular and Biochemical Parasitology 1987, 24:55-66.
6. Dókušová L., Sirági P., Klement C., Schréter I., Kristian P., Jarčuška P., Virág L. Confirmation of anthrax occurrence using real-time PCR. Biologia 2004, 59:803-807.
7. Kilvington S., Beeching J. Development of a PCR for identification of Naegleria fowleri from the environment. Applied and Environmental Microbiology 1995, 61:3764-3767.
8. Kilvington S., Beeching J. Identification and epidemiological typing of Naegleria fowleri with DNA probes. Applied and Environmental Microbiology 1995, 61:2071-2078.
9. Kowalski R.P., Thompson P.P., Kinchington P.R., Gordon Y.J. Evaluation of the Smart Cycler II system for real-time detection of viruses and Chlamydia from ocular specimens. Archives of Ophthalmology 2006, 124:1135-1139.
10. MacLean R., Richardson D.J., LePardo R., Marciano-Cabral F. The Identification of Naegleria fowleri from water and soil samples by nested PCR. Parasitology Research 2004, 93:211-217.
11. Marciano-Cabral F. Free-living amoebae as agents of human infection. The Journal of Infectious Diseases 2009, 199:1104-1106.
12. Marciano-Cabral F. Biology of Naegleria spp. Microbiology and Molecular Biology Reviews 1988, 52:114-133.
13. Marciano-Cabral F., MacLean R., Mensah A., LaPat-Polasko L. Identification of a Naegleria fowleri in domestic water sources by nested PCR. Applied and Environmental Microbiology 2003, 69:5864-5869.
14. McLaughlin G.L., Brandt F.H., Visvesvara G.S. Restriction fragment length polymorphisms of the DNA of selected Naegleria and Acanthamoeba amoebae. Journal of Clinical Microbiology 1988, 26:1655-1658.
15. McLaughlin G.L., Vodkin M.H., Huizinga H.W. Amplification of repetitive DNA for the specific detection of Naegleria fowleri. Journal of Clinical Microbiology 1991, 29:227-230.
16. Milligan S.M., Band R.N. Restriction endonuclease analysis of mitochondrial DNA as an aid in the taxonomy of Naegleria and Vahlkampfia. Journal of Protozoology 1988, 35:198-204.
17. Pélandakis M., Pernin P. Use of multiplex PCR and PCR restriction enzyme analysis for detection and exploration of the variability in the free-living amoeba Naegleria in the environment. Applied and Environmental Microbiology 2002, 68:2061-2065.
18. Piatek A.S., Tyagi S., Pol A.C., Telenti A., Miller L.P., Kramer F.R., Alland D. Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis. Nature Biotechnology 1998, 16:359-363.
19. Qvarnstrom Y., Visvesvara G.S., Sriram R., da Silva A.J. Multiplex real-time PCR assay for simultaneous detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri. Journal of Clinical Microbiology 2006, 44:3589-3595.
20. Réveiller F.L., Cabanes P.A., Marciano-Cabral F. Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri. Parasitology Research 2002, 88:443-450.
21. Réveiller F.L., Suh S.L., Sullivan K., Cabanes P.A., Marciano-Cabral F. Isolation of a unique membrane protein from Naegleria fowleri. The Journal of Eukaryotic Microbiology 2001, 48:676-682.
22. Ririe K.M., Rasmussen R.P., Wittwer C.T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Analytical Biochemistry 1997, 245:154-160.
23. Robinson B.S., Monis P.T., Dobson P.J. Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis. Applied and Environmental Microbiology 2006, 72:5857-5863.
24. Sheehan K.B., Faqq J.A., Ferris M.J., Henson J.M. PCR detection and analysis of the free-living amoeba Naegleria in hot springs in yellowstone and Grand Teton National Parks. Applied and Environmental Microbiology 2003, 69:5914-5918.
25. Schild M., Gianinazzi Ch., Gottstein B., Muller N. PCR-based diagnosis of Naegleria sp. Infection in formalin-fixed and paraffin-embedded brain sections. Journal of Clinical Microbiology 2007, 45:564-567.
26. Schuster F.L. Cultivation of pathogenic and opportunistic free-living amebas. Clinical Microbiology Reviews 2002, 15:342-354.
27. Sparagano O. Detection of Naegleria fowleri cysts in environmental samples by using aDNA probe. FEMS Microbiology Letters 1993, 112:349-351.
28. Sparagano O. Differentiation of Naegleria fowleri and other Naegleria by polymerase chain reaction and hybridization methods. FEMS Microbiology Letters 1993, 110:325-330.
29. Trnková K., Maďarová L., Klement C. Primary amoebic meningoencephalitis (Naegleriasis) - problem of public health?. Hygiena 2008, 53:53-59.
30. van Belkum A., De Jonckheere J., Quint W.G. Genotyping Naegleria spp. and Naegleria fowleri isolates by interrepeat polymerase chain reaction. Journal of Clinical Microbiology 1992, 30:2595-2598.
31. Visvesvara G.S. Pathogenic and opportunistic free living amebas. Manual of Clinical Microbiology 1999, 1383-1390. ASM Press, Washington, DC. seventh ed. P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, R.H. Yolken (Eds.).
32. Visvesvara G.S., Moura H., Schuster F.L. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunology and Medical Microbiology 2007, 50:1-26.