Use of multiplex PCR and PCR restriction enzyme analysis for detection and exploration of the variability in the free-living amoeba Naegleria in the environment

Applied and Environmental Microbiology

Volumn 68, Issue 4, 2002, Pages 2061-2065

  Pélandakis, Michel ^a   Pernin, Pierre ^a  

^a UNIVERSITÉ LYON 1   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIODIVERSITY; BIOSENSORS; SAMPLING;

POLYMORPHISM;

ENZYMES;

DETECTION METHOD;

AMOEBA; ARTICLE; ENVIRONMENT; GEOGRAPHIC DISTRIBUTION; MOLECULAR BIOLOGY; NAEGLERIA FOWLERI; NONHUMAN; PARASITE ISOLATION; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RESTRICTION MAPPING;

ANIMALS; DNA, PROTOZOAN; DNA, RIBOSOMAL SPACER; NAEGLERIA; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SPECIES SPECIFICITY; WATER;

NAEGLERIA; NAEGLERIA FOWLERI; PROTOZOA;

EID: 0036206280     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.4.2061-2065.2002     Document Type: Article

References (29)

1. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis
2. Unexplored reservoirs of pathogenic bacteria: Protozoa and biofilms
3. Description of a Naegleria sp. isolated from two cases of primary amoebic meningo-encephalitis, and of the experimental pathological changes induced by it
4. Naegleria fowleri in cooling waters of power plants
5. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications
6. Evaluation of evolutionary divergence in the genus Naegleria by analysis of ribosomal DNA plasmid restriction patterns
7. A comparative study of 14 strains of Naegleria australiensis demonstrates the existence of a highly virulent subspecies: N. australiensis, italica n. spp.
8. A group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification
9. Riboprinting of Naegleria spp.: Small-subunit versus large-subunit rDNA
10. Sequence variation in the ribosomal internal transcribed spacers, including the 5.8S rDNA, of Naegleria spp.
11. Isolation of Naegleria fowleri from the cooling pond of an electric power plant in France
12. Thermal ecology of Naegleria fowleri from a power plant cooling reservoir
13. Seasonal distribution of pathogenic free-living amebae in Oklahoma waters
14. Development of a PCR for identification of Naegleria fowleri from the environment
15. Waterborne protozoan pathogens
16. A quantitative model of intracellular growth of Legionella pneumophila in Acanthamoeba castellanii
17. Taxonomic criteria for limax amoebae, with descriptions of 3 new species of Hartmannella and 3 of Vahlkampfia
18. DNA diversity among the free-living amoeba Naegleria fowleri detected by the RAPD method
19. Genetic variation in the free-living amoeba Naegleria fowleri
20. Analysis of the 5.8S rRNA gene and the internal transcribed spacers in Naegleria spp. and in N. fowleri
21. Biochemical identification and phylogenetic relationships in free-living amoebas of the genus Naegleria
22. Application of isoenzymatic typing to the identification of nonaxenic strains of Naegleria (Protozoa, Rhizopoda)
23. Appearance in Europe of Naegleria fowleri displaying the Australian type of restriction-fragment-length-polymorphism
24. Genetic structure of natural populations of the free-living amoeba Naegleria lovaniensis. Evidence for sexual reproduction
25. Use of monoclonal antibodies to distinguish pathogenic Naegleria fowleri (cysts, trophozoites, or flagellate forms) from other Naegleria species
26. Isolation of the freshwater Naegleria fowleri (Carter, 1970) from a river using a monoclonal antibody and the polymerase chain reaction
27. Occurrence and pathogenicity of Naegleria fowleri in artificially heated waters
28. Effects of thermal additions on the density and distribution of thermophilic amoebae and pathogenic Naegleria fowleri in a newly created cooling lake
29. Multiplication of Legionella spp. in tap water containing Hanmannella vermiformis