Quantitative detection and differentiation of free-living amoeba species using SYBR green-based real-time PCR melting curve analysis

Current Microbiology

Volumn 53, Issue 6, 2006, Pages 506-509

  Behets, Jonas ^a   Declerck, Priscilla ^a   Delaedt, Yasmine ^a   Verelst, Lieve ^b   Ollevier, Frans ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b LABORELEC   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR GEL ELECTROPHORESIS; AMOEBA; AMPLICON; ARTICLE; CULTURE MEDIUM; FREE LIVING AMOEBA; GENE AMPLIFICATION; HARTMANELLA VERMIFORMIS; MELTING CURVE ANALYSIS; NAEGLERIA AUSTRALIENSIS; NAEGLERIA FOWLERI; NAEGLERIA GRUBERI; NAEGLERIA LOVANIENSIS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SARCODINA; SEQUENCE ANALYSIS; SPECIES DIFFERENTIATION; WILLAERTIA MAGNA;

ACANTHAMOEBA CASTELLANII; AMOEBIDA; ANIMALS; HARTMANNELLA; NAEGLERIA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY;

HARTMANNELLA VERMIFORMIS; NAEGLERIA FOWLERI; NAEGLERIA GRUBERI; WILLAERTIA MAGNA;

EID: 33751519531     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-006-0241-0     Document Type: Article

References (23)

1. Aguilera A, Gomez F, Lospitao E, Amils R (2006) A molecular approach to the characterization of the eukaryotic communities of an extreme acidic environment: Methods for DNA extraction and denaturing gradient gel electrophoresis analysis. Syst Appl Microbiol 29:593-605
2. Beattie TK, Tomlinson A, Mcfayden AK, Seal DV (2002) Most probable number method of amoebal enumeration in the determination of the amoebicidal activity of multi-purpose solutions. Invest Ophthalmol Vis Sci 43:2992-3000
3. Behets J, Declerck P, Delaedt Y, Verelst L, Ollevier F (2006) A duplex real-time PCR assay for the quantitative detection of Naegleria fowleri in water samples. Water Res (accepted)
4. Behets J, Seghi F, Declerck P, Verelst L, Duvivier L, Van Damme A, Ollevier F (2003) Detection of Naegleria spp. and Naegleria fowleri: a comparison of flagellation tests, ELISA and PCR. Water Sci Technol 47:117-122
5. Clark CG (1990) Genome structure and evolution of Naegleria and its relatives. J Protozool 37:S2-S6
6. Giglio S, Monis PT, Saint CP (2005) Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology. Appl Environ Microbiol 71:8944-8948
7. Kilvington S, Beeching J (1995) Development of a PCR for identification of Naegleria fowleri from the environment. Appl Environ Microbiol 61:3764-3767
8. Limor JR, Lal AA, Xiao LH (2002) Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR. J Clin Microbiol 40:2335-2338
9. Marciano-Cabral F, Maclean R, Mensah A, Lapat-Polasko L (2003) Identification of Naegleria fowleri in domestic water sources by nested PCR. Appl Environ Microbiol 69:5864-5869
10. Monis PT, Giglio S, Keegan AR, Thompson RCA (2005) Emerging technologies for the detection and genetic characterization of protozoan parasites. Trends Parasitol 21:340-346
11. Monis PT, Giglio S, Saint CP (2005) Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis. Anal Biochem 340:24-34
12. Ortega-Rivas A, Lorenzo-Morales J, Martinez-Carretero E, Lloberas MV, Hernandez BV, Del Castillo-Remiro A (2004) Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga. Curr Microbiol 48:360-363
13. Pelandakis M, Pernin P (2002) Use of multiplex PCR and PCR restriction enzyme analysis for detection and exploration of the variability in the free-living amoeba Naegleria in the environment. Appl Environ Microbiol 68:2061-2065
14. Pelandakis M, Serre S, Pernin P (2000) Analysis of the 5.8S rRNA gene and the internal transcribed spacers in Naegleria spp. and in N. fowleri. J Eukaryot Microbiol 47:116-121
15. Riviere D, Szczebara FM, Berjeaud JM, Frere J, Hechard Y (2006) Development of a real-time PCR assay for quantification of Acanthamoeba trophozoites and cysts. J Microbiol Methods 64:78-83
16. Rodriguez-Zaragoza S (1994) Ecology of free-living amoebae. Crit Rev Microbiol 20:225-241
17. Schroeder JM, Booton GC, Hay J, Niszl IA, Seal DV, Markus MB, Fuerst PA, Byers TJ (2001) Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge. J Clin Microbiol 39:1903-1911
18. Schuster FL, Visvesvara GS (2004) Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol 34:1001-1027
19. Seurinck S, Defoirdt T, Verstraete W, Siciliano SD (2005) Detection and quantification of the human-specific HF183 bacteroides 16S rRNA genetic marker with real-time PCR for assessment of human faecal pollution in freshwater. Environ Microbiol 7:249-259
20. Sheehan KB, Fagg JA, Ferris MJ, Henson JM (2003) PCR detection and analysis of the free-living amoeba Naegleria in hot springs in Yellowstone and Grand Teton National Parks. Appl Environ Microbiol 69:5914-5918
21. Suzan-Monti M, La Scola B, Raoult D (2006) Genomic and evolutionary aspects of Mimivirus. Virus Res 117:145-155
22. Swanson MS, Hammer BK (2000) Legionella pneumophila pathogenesis: a fateful journey from amoebae to macrophages. Annu Rev Microbiol 54:567-613
23. Tanriverdi S, Tanyeli A, Baslamisli F, Koksal F, Kilinc Y, Feng XC, Batzer G, Tzipori S, Widmer G (2002) Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis. J Clin Microbiol 40:3237-3244