Evaluation of the SmartCycler II system for real-time detection of viruses and Chlamydia from ocular specimens

Archives of Ophthalmology

Volumn 124, Issue 8, 2006, Pages 1135-1139

  Kowalski, Regis P ^a,b   Thompson, Paul P ^a   Kinchington, Paul R ^a   Gordon, Y Jerold ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b University of Pittsburgh School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; VIRUS DNA;

ADENOVIRUS; ARTICLE; BACTERIAL EYE INFECTION; CELL CULTURE; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; HERPES SIMPLEX VIRUS 1; HUMAN; HUMAN CELL; METHODOLOGY; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VARICELLA ZOSTER VIRUS; VIRAL EYE INFECTION; VIRUS DETECTION;

ADENOVIRUSES, HUMAN; CHLAMYDIA TRACHOMATIS; DNA, BACTERIAL; DNA, VIRAL; EYE INFECTIONS, BACTERIAL; EYE INFECTIONS, VIRAL; FALSE POSITIVE REACTIONS; HERPESVIRUS 1, HUMAN; HERPESVIRUS 3, HUMAN; HUMANS; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; VIRUSES;

EID: 33747162136     PISSN: 00039950     EISSN: 00039950     Source Type: Journal    
DOI: 10.1001/archopht.124.8.1135     Document Type: Article

References (11)

1. Gingeras TR, Higuchi R, Kricka LJ, Lo YM, Wittwer CT. Fifty years of molecular (DNA/RNA) diagnostics. Clin Chem. 2005;51:661-671.
2. Kowalski RP, Gordon YJ, Romanowski EG, Araullo-Cruz T, Kinchington PR. A comparison of enzyme immunoassay and polymerase chain reaction with the clinical examination for diagnosing ocular herpetic disease. Ophthalmology. 1993;100:530-533.
3. Kinchington PR, Turse SE, Kowalski RP, Gordon YJ. Use of polymerase chain amplification for the detection of adenovirus in ocular swab specimens. Invest Ophthalmol Vis Sci. 1994;35:4126-4134.
4. Kowalski RP, Uhrin M, Karenchak LM, Sweet RL, Gordon YJ. Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis. Ophthalmology. 1995;102:1016-1019.
5. Kowalski RP, Karenchak LM, Gordon YJ. The evaluation of the shell vial technique for the detection of ocular adenovirus. Ophthalmology. 1999;106:1324-1327.
6. Kowalski RP, Karenchak LM, Gordon JS. The evaluation of the ELVIS system for detecting herpes simplex virus from ocular samples. Arch Ophthalmol. 2002;120:960-962.
7. Heim A, Ebnet C, Harste G, Pring-Akerblom P. Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J Med Virol. 2003;70:228-239.
8. Weidmann M, Meyer-Konig U, Hufert F. Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR. J Clin Microbiol. 2003;41:1565-1568.
9. Watzinger F, Suda M, Preuner S, et al. Real-time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients. J Clin Microbiol. 2004;42:5189-5198.
10. Pickett MA, Everson JS, Pead PJ, Clarke IN. The plasmids of Chlamydia trachomatis and Chlamydophila pneumoniae (N16): accurate determination of copy number and the paradoxical effect of plasmid-curing agents. Microbiology. 2005;151:893-903.
11. Koidl C, Bozic M, Mossbock G, et al. Rapid diagnosis of adenoviral keratoconjunctivitis by a fully automated molecular assay. Ophthalmology. 2005;112:1521-1527.