Mutagenesis of klebsiella aerogenes UreG to probe nickel binding and interactions with other urease-related proteins

Biochemistry

Volumn 49, Issue 28, 2010, Pages 5859-5869

  Boer, Jodi L ^a   Quiroz Valenzuela, Soledad ^a,b   Anderson, Kimberly L ^c   Hausinger, Robert P ^a,c  

^a Michigan State University   (United States)

^b Universidad Santo Tomás ^*  (United States)

^c UNIVERSIDAD SANTO TOMÁS   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; CHARGE-TRANSFER BANDS; DEPENDENT PROCESS; HYDROGENASES; IN-VITRO; IN-VIVO; KLEBSIELLA; METAL LIGANDS; MUTANT PROTEINS; MUTATION RESULTS; NICKEL IONS; PROTEIN-PROTEIN INTERACTIONS; PURIFIED PROTEIN; RECOMBINANT ESCHERICHIA COLI; THIOLATES; ZINC IONS;

ACTIVATION ANALYSIS; BINDING ENERGY; ENZYME ACTIVITY; ESCHERICHIA COLI; METAL IONS; METAL REFINING; NICKEL; NICKEL ALLOYS; ZINC;

PROTEINS;

BACTERIAL ENZYME; METALLOCHAPERONE; NICKEL; UREASE; ZINC ION;

ARTICLE; BACTERIAL CELL; BINDING SITE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ESCHERICHIA COLI; IN VITRO STUDY; IN VIVO STUDY; KLEBSIELLA AEROGENES; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

BINDING SITES; ENTEROBACTER; ENTEROBACTER AEROGENES; ESCHERICHIA COLI; GUANOSINE TRIPHOSPHATE; HYDROGENASE; KLEBSIELLA; METALLOCHAPERONES; METALS; MUTAGENESIS; NICKEL; PROTEINS; UREASE; ZINC;

ESCHERICHIA COLI; KLEBSIELLA AEROGENES;

EID: 77954583264     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi1004987     Document Type: Article

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