Purification and activation properties of UreD-UreF-Urease apoprotein complexes

Journal of Bacteriology

Volumn 178, Issue 18, 1996, Pages 5417-5421

  Moncrief, Mary Beth C ^a   Hausinger, Robert P ^a,b  

^a Michigan State University   (United States)

^b NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

UREASE;

AMINO ACID SUBSTITUTION; ARTICLE; COMPLEX FORMATION; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME SUBUNIT; GENE DELETION; GENE LOCATION; KLEBSIELLA AEROGENES; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN STRUCTURE; RIBOSOME SUBUNIT;

KLEBSIELLA AEROGENES;

EID: 0029809744     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.18.5417-5421.1996     Document Type: Article

References (19)

1. Blake, M. S., K. H. Johnsion, G. L. Russel-Jones, and E. C. Golschlich. 1984. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal. Biochem. 136:175-179.
2. Hansinger, R. P. 1993. Urease, p. 23-57. In Biochemistry of nickel. Plenum Publishing Corp., New York.
3. Jabri, E., M. B. Carr, R. P. Hausinger, and P. A. Karplus. 1995. The crystal structure of urease from Klebsiella aerogenes. Science 268:998-1004.
4. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
5. Lee, M. H., S. B. Mulrooney, M. J. Renner, Y. Markowicz, and R. P. Hausinger. 1992. Sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis. J. Bacteriol. 174:4324-4330.
6. Lee, M. H., H. S. Pankratz, S. Wang, R. A. Scott, M. G. Finnegan, M. K. Johnson, J. A. Ippolito, D. W. Christianson, and R. P. Hansinger. 1993. Purification and characterization of Klebsiella aerogenes UreE protein: a nickel-binding protein that functions in urease metallocenter assembly. Protein Sci. 2:1042-1052.
7. Mobley, H. L. T., M. D. Island, and R. P. Hausinger. 1995. Molecular biology of microbial ureases. Microbiol. Rev. 59:451-480.
8. Moncrief, M. B. C., and R. P. Hausinger. 1996. Nickel incorporation into urease, p. 151-171. In R. P. Hausinger, G. L. Eichhorn, and L. G. Marzilli (ed.), Mechanisms of metallocenter assembly. VCH Publishers, New York.
9. Mulrooney, S. B., and R. P. Hausinger. 1990. Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation. J. Bacteriol. 172:5837-5843.
10. Mulrooney, S. B., H. S. Pankratz, and R. P. Hausinger. 1989. Regulation of gene expression and cellular localization of cloned Klebsiella aerogenes (K. pneumoniae) urease. J. Gen. Microbiol. 135:1769-1776.
11. Park, I.-S. 1994. Ph.D. thesis. Michigan State University, East Lansing.
12. Park, I.-S., M. B. Carr, and R. P. Hausinger. 1994. In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly. Proc. Natl. Acad. Sci. USA 91:3233-3237.
13. Park, I.-S., and R. P. Hausinger. 1995. Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation. J. Bacteriol. 177:1947-1951.
14. Park, I.-S., and R. P. Hausinger. 1995. Requirement of carbon dioxide for in vitro assembly of the urease nickel metallocenter. Science 267:1156-1158.
15. Park, I.-S., and R. P. Hausinger. 1996. Metal ion interactions with urease and UreD-urcase apoproteins. Biochemistry 35:5345-5352.
16. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
17. Taha, T., and R. P. Hausinger. 1996. Unpublished observations.
18. Todd, M. J., and R. P. Hausinger. 1987. Purification and characterization of the nickel-containing multicomponent urease from Klebsiella aerogenes. J. Biol. Chem. 262:5963-5967.
19. Weatherburn, M. W. 1967. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 39:971-974.