Molecular characterization of T-DNA integration sites in transgenic birch

Trees - Structure and Function

Volumn 24, Issue 4, 2010, Pages 753-762

  Zeng, Fan Suo ^a   Zhan, Ya Guang ^a   Zhao, Hong Cui ^a   Xin, Ying ^a   Qi, Feng Hui ^a   Yang, Chuan Ping ^a  

^a NORTHEAST FORESTRY UNIVERSITY   (China)

Author keywords

Flanking sequences; Integration sites; Transgenic birch (Betula platyphylla Suk.); Vector backbone DNA

Indexed keywords

AGROBACTERIUM TUMEFACIENS; COPY NUMBER; DNA REPEATS; DNA TRANSPORT; FLANKING REGIONS; FLANKING SEQUENCE; FLANKING SEQUENCES; HOMOLOGOUS SEQUENCES; MOLECULAR CHARACTERIZATION; RESISTANCE GENES; TRANSGENE; TRANSGENE EXPRESSION; TRANSGENIC BIRCH (BETULA PLATYPHYLLA SUK.); TRANSGENIC LINES; TRANSGENIC PLANTS; TRANSGENICS; TREE SPECIES;

DNA; GENE ENCODING; INTEGRATION; VECTORS;

DNA SEQUENCES;

AGROBACTERIUM TUMEFACIENS; BETULA PLATYPHYLLA; HEXAPODA;

EID: 77954537142     PISSN: 09311890     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00468-010-0445-6     Document Type: Article

References (43)

1. Abdal-Aziz SA, Pliego-Alfaro F, Quesada MA, Mercado JA (2006) Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant. J Biosci Bioeng 6: 508-510.
2. Antal Z, Rascle C, Fevre M, Bruel C (2004) Single oligonucleotide nested PCR: a rapid method for the isolation of genes and their flanking regions from expressed sequence tags. Curr Genet 46: 240-246.
3. Brunaud V, Balzergue S, Dubreucq B, Aubourg S, Samson F, Chauvin S, Bechtold N, Cruaud C, DeRose R, Pelletier G, Lepiniec L, Caboche M, Lecharny A (2002) T-DNA integration into the Arabidopsis genome depends on sequences of pre-insertion sites. EMBO Rep 12: 1152-1157.
4. Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol 115: 971-980.
5. Chilton MD, Que Q (2003) Targeted integration of T-DNA into the tobacco genome at double-stranded breaks: new insights on the mechanism of T-DNA integration. Plant Physiol 133: 956-965.
6. De Buck S, Jacobs A, van Montagu M, Depicker A (1999) The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration. Plant J 20: 295-304.
7. De Buck S, De Wilde C, van Montagu M, Depicker A (2000) Determination of the T-DNA transfer and the T-DNA integration frequencies upon cocultivation of Arabidopsis thaliana root explants. Mol Plant Microbe Interact 13: 658-665.
8. Gelvin SB (1998) The introduction and expression of transgenes in plants. Curr Opin Biotechnol 9: 227-232.
9. Gelvin SB (2000) Agrobacterium and plant genes involved in T-DNA transfer and integration. Annu Rev Plant Physiol Plant Mol Biol 51: 223-256.
10. Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool. Microbiol Mol Biol Rev 67: 16-37.
11. Hooykaas PJJ, Beijersbergen AGM (1994) The virulence system of Agrobacterium tumefaciens. Annu Rev Phytopathol 32: 157-179.
12. Iglesias VA, Moscone EA, Papp I, Neuhuber F, Michalowski S, Phelan T, Spiker S, Matzke M, Matzke AJM (1997) Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco. Plant Cell 9: 1251-1264.
13. Keim M, Williams RS, Harwood AJ (2004) An inverse PCR technique to rapidly isolate the flanking DNA of dictyostelium insertion mutants. Mol Biotechnol 26: 221-224.
14. Kim SR, Lee J, Jun SH, Park S, Kang HG, Kwon S, Gynheung A (2003) Transgene structures in T-DNA-inserted rice plants. Plant Mol Biol 52: 761-773.
15. Kohli A, Leech M, Vain P, Laurie DA, Christou P (1998) Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci USA 95: 7203-7208.
16. Kumar S, Fladung M (2001) Gene stability in transgenic aspen (Populus). II. Molecular characterization of variable expression of transgene in wild and hybrid aspen. Planta 213: 731-740.
17. Kumar S, Fladung M (2002) Transgene integration in aspen: structures of integration sites and mechanism of T-DNA integration. Plant J 31: 543-551.
18. Kuraya Y, Ohta S, Fukude M (2004) Suppression of transfer of non-T-DNA 'vector backbone' sequences by multiple left border repeats in vectors for transformation of higher plants mediated by Agrobacterium tumefaciens. Mol Breed 14: 309-320.
19. Lange M, Vincze E, Moller MG, Holm PB (2006) Molecular analysis of transgene and vector backbone integration into the barley genome following Agrobacterium-mediated transformation. Plant Cell Rep 25: 815-820.
20. Liu YG, Mitsukawa N, Oosumi T, Whittier RF (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8: 457-463.
21. Mayerhofer R, Koncz-Kalman Z, Nawrath C, Bakkeren G, Crameri A, Angelis K, Redei GP, Schell J, Hohn B, Koncz C (1991) T-DNA integration: a mode of illegitimate recombination in plants. EMBO J 10: 697-704.
22. Olhoft PM, Flagel LE, Somers DAT (2004) DNA locus structure in a large population of soybean plants transformed using the Agrobacterium-mediated cotyledonary node method. Plant Biotechnol J 2: 289-300.
23. Riley J, Butler R, Ogilvie D, Finniear R, Jenner D, Powell S, Anand R, Smith JC, Markham AF (1990) A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res 18: 2887-2890.
24. Salomon S, Puchta H (1998) Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells. EMBO J 17: 6086-6095.
25. Sheng J, Citovsky V (1996) Agrobacterium-plant cell DNA transport: have virulence proteins, will travel. Plant Cell 8: 1699-1710.
26. Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA (1995) An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res 23: 1087-1088.
27. Stachel SE, Zambryski P (1989) Generic trans-kingdom sex. Nature 340: 190-191.
28. Takano M, Egawa H, Ikeda JE, Wakasa K (1997) The structures of integration sites in transgenic rice. Plant J 11: 353-361.
29. Tan G, Gao Y, Shi M, Zhang X, He S, Chen Z, An C (2005) SiteFinding-PCR: a simple and efficient PCR method for chromosome walking. Nucleic Acids Res 33: e122.
30. Tang W, Newton RJ, Weidner DA (2007) Genetic transformation and gene silencing mediated by multiple copies of a transgene in eastern white pine. J Exp Bot 58: 545-554.
31. Tzfira T, Citovsky V (2000) From host recognition to T-DNA integration: the function of bacterial and plant genes in the Agrobacterium plant cell interaction. Mol Plant Pathol 1: 201-212.
32. Tzfira T, Frankmen L, Vaidya M, Citovsky V (2003) Site-specific integration of Agrobacterium T-DNA via double-stranded intermediates. Plant Physiol 133: 1011-1023.
33. van Der Graaff E, Den Dulk-Ras A, Hooykaas PJJ (1996) Deviating T-DNA transfer from Agrobacterium tumefaciens to plants. Plant Mol Biol 31: 677-681.
34. Wenck A, Czako M, Kanevski I, Marton L (1997) Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation. Plant Mol Biol 34: 913-922.
35. Wood DW, Setubal JC, Kaul R, Monks DE, Kitajima JP, Okura VK, Zhou Y, Chen L, Wood GE, Almeida NF et al (2001) The genome of the natural genetic engineer Agrobacterium tumefaciens C58. Science 294: 2317-2323.
36. Yan YX, An CC, Li L, Gui JY, Tan GH, Cen ZL (2003) T-linker-specific ligation PCR (T-linker PCR): an advanced PCR technique for chromosome walking or for isolation of tagged DNA ends. Nucleic Acids Res 31: e68.
37. Yin Z, Wang GL (2000) Evidence of multiple complex patterns of T-DNA integration into the rice genome. Theor Appl Genet 100: 461-470.
38. Zambryski P (1992) Chronicles from the Agrobacterium-plant cell DNA transfer story. Annu Rev Plant Physiol Plant Mol Biol 43: 465-490.
39. Zeng FS, Zhan YG, Nan N, Xin Y, Qi FH, Yang CP (2009) Expression of BGT gene in Transgenic Birch (Betula platyphylla). Afr J Biotechnol 8: 3392-3398.
40. Zhai W, Chen C, Zhu X, Chen X, Zhang D, Li X, Zhu L (2004) Analysis of T-DNA Xa21 loci and bacterial blight resistance effects of the transgene Xa21 in transgenic rice. Theor Appl Genet 109: 534-542.
41. Zhang J, Cai L, Cheng J, Mao H, Fan X, Meng Z, Chan KM, Zhang H, Qi J, Ji L, Hong Y (2008) Transgene integration and organization in cotton (Gossypium hirsutum L.) genome. Transgenic Res 17: 293-306.
42. Ziemienowicz A, Tinland B, Bryant J, Gloeckler V, Hohn B (2000) Plant enzymes but not Agrobacterium VirD2 mediate T-DNA ligation in vitro. Mol Cell Biol 20: 6317-6322.
43. Zupan J, Muth TR, Draper O, Zambryski P (2000) The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights. Plant J 23: 11-28.