Suppression of transfer of non-T-DNA 'vector backbone' sequences by multiple left border repeats in vectors for transformation of higher plants mediated by Agrobacterium tumefaciens

Molecular Breeding

Volumn 14, Issue 3, 2004, Pages 309-320

  Kuraya, Yoshiki ^a,b   Ohta, Shozo ^a,b   Fukuda, Miyuki ^b   Hiei, Yukoh ^a,b,c   Murai, Nobuhiko ^a,b   Hamada, Kazuyuki ^a,b,c   Ueki, Jun ^a,b,c   Imaseki, Hidemasa ^a,b   Komari, Toshihiko ^a,b,c  

^a JT Plant Breeding and Genetics Research Laboratory   (Japan)

^b Orynova KK   (Japan)

^c JT Plant Innovation Center   (Japan)

Author keywords

Agrobacterium; Multiple left border repeats; Rice; T DNA; Transformation; Vector backbone

Indexed keywords

BACTERIA; DNA SEQUENCES; GENE EXPRESSION; PLANTS (BOTANY); POLYMERASE CHAIN REACTION; VECTORS;

AGROBACTERIA; HIGHER PLANTS; MULTIPLE LEAVE BORDER REPEAT; MULTIPLE LEAVES; RICE; T-DNA; TRANSFORMANTS; TRANSFORMATION; TUMEFACIENS; VECTOR BACKBONE;

DNA;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; EMBRYOPHYTA; RHIZOBIUM;

EID: 33744962727     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:MOLB.0000047792.77219.bb     Document Type: Article

References (29)

1. Barker R.F., Idler K.B., Thompson D.V. and Kemp J.D. 1983. Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol. Biol. 2: 335-390.
2. Christensen A.H., Sharrock R.A. and Quail P.H. 1992. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol. Biol. 18: 675-689.
3. Cotsaftis O., Sallaud C., Breitler J.C., Meynard D., Greco R., Pereira A. and Guiderdoni E. 2002. Transposon-mediated generation of T-DNA- and marker-free rice plants expressing a Bt endotoxin gene. Mol Breed. 10: 165-180.
4. De Buck S., De Wilde C., van Montagu M. and Depicker A. 2000. T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformation. Mol Breed. 6: 459-468.
5. Depicker A., Stachel S., Dhaese P., Zambryski P. and Goodman H.M. 1982. Nopaline synthase: transcript mapping and DNA sequence. J. Mol. Appl. Genet. 1: 561-573.
6. Guillemaunt P. 1992. Isolation of plant DNA: a fast, inexpensive, and reliable method. Plant Mol. Biol. Rep. 10: 60-65.
7. Hanson B., Engler D., Moy Y., Newman B., Ralston E. and Gutterson N. 1999. A simple method to enrich an Agrobacterium- transformed population for plants containing only T-DNA sequences. Plant J. 19: 727-734.
8. Hiei Y., Ohta S., Komari T. and Kumashiro T. 1994. Efficient transformation of rice Oryza sativa L. mediated by Agrobacterium and sequence analysis of the boundaries of T-DNA. Plant J. 6: 271-282.
9. Hooykaas P.J.J. and Beijersbergen A.G.M. 1994. The virulence system of Agrobacterium tumefaciens. Ann Rev Phytopathol. 32: 157-179.
10. Ishida Y., Saito H., Ohta S., Hiei Y., Komari T. and Kumashiro T. 1996. High efficiency transformation of maize Zea mays L. mediated by Agrobacterium tumefaciens. Nature Biotechnol. 14: 745-750.
11. Komari T. 1990. Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542. Plant Cell Rep. 9: 303-306.
12. Komari T. and Kubo T. 1999. Methods of Genetic Transformation: Agrobacterium tumefaciens. In: Vasil I.K. ed., Molecular Improvement of Cereal Crops. Kluwer Academic, Dordrecht, The Netherlands, pp. 43-82.
13. Komari T., Hiei Y., Saito Y., Murai N. and Kumashiro T. 1996. Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J. 10: 165-174.
14. Kononov M.E., Bassuner B. and Gelvin S.B. 1997. Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: evidence for multiple complex patterns of integration. Plant J. 11: 945-957.
15. Lu H.- J., Zhou X.-R., Gong Z.-X. and Upadhyaya N.M. 2001. Generation of selectable marker-free transgenic rice using double right-border DRB binary vectors. Aust. J. Plant Physiol. 28: 241-248.
16. Martineau B., Voelker T.A. and Sanders R.A. 1994. On defining T-DNA. Plant Cell. 6: 1032-1033.
17. Maruta Y., Ueki J., Saito H., Nitta N. and Imaseki H. 2001. Transgenic rice with reduced glutelin content by transformation with glutelin A antisense gene. Mol Breed. 8: 273-284.
18. Miller M., Tagliani L., Wang N., Berka B., Bidney D. and Zhao Z.- Y. 2002. High efficiency transgene segregation in co-trans- formed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system. Transgenic Res. 11: 381-396.
19. Ohta S., Mita S., Hattori T. and Nakamura K. 1990. Construction and expression in tobacco of a beta-glucuronidase GUS reporter gene containing an intron within the coding sequence. Plant Cell Physiol. 31: 805-813.
20. Ow D.W., Wood K., DeLuca M., De Wet J.R., Helinski D.R. and Howell S. 1986. Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science. 234: 856-859.
21. Ramanathan V. and Veluthambi K. 1995. Transfer of non-T-DNA portions of the Agrobacterium tumefaciens Ti plasmid from the left terminus of TL-DNA. Plant Mol. Biol. 28: 1149-1154.
22. Sallaud C., Meynard D., van Boxtel J., Gay C., Bès M., Brizard J.P., Larmande P., Ortega D., Raynal M., Portefaix M., Ouwerkerk P.B.F., Rueb S., Delseny M. and Guiderdoni E. 2003. Highly efficient production and characterization of T-DNA plants for rice Oryza sativa L. functional genomics. Theor. Appl. Genet. 106: 1396-1408.
23. Sambrook J. and Russell D.W. 2001. Molecular Cloning, A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA.
24. Sheng J. and Citovsky V. 1996. Agrobacterium-plant cell DNA transport: Have virulence proteins, will travel. Plant Cell. 8: 1699-1710.
25. Vain P., Afolabi A.S., Worland B. and Snape J.W. 2003. Transgene behavior in populations of rice plants transformed using a new dual binary vector system: pGreen/pSoup. Theor Appl Genet. 107: 210-217.
26. van der Graaff E., den Dulk-Ras A. and Hooykaas P.J.J. 1996. Deviating T-DNA transfer from Agrobacterium tumefaciens to plants. Plant Mol. Biol. 31: 677-681.
27. Yin Z. and Wang G.- L. 2000. Evidence of multiple complex patterns of T-DNA integration into the rice genome. Theor Appl. Genet. 100: 461-470.
28. Zambryski P., Depicker A., Kruger K. and Goodman H.M. 1982. Tumor induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA. J. Mol. Appl. Genet. 1: 361-370.
29. Zupan J., Muth T.R., Draper O. and Zambryski P. 2000. The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights. Plant J. 23: 11-28.