Single oligonucleotide nested PCR: A rapid method for the isolation of genes and their flanking regions from expressed sequence tags

Current Genetics

Volumn 46, Issue 4, 2004, Pages 240-246

  Antal, Zsuzsanna ^a,b   Rascle, Christine ^a   Févre, Michel ^a   Bruel, Christophe ^a  

^a UNIVERSITÉ DE LYON   (France)

^b UNIVERSITY OF SZEGED   (Hungary)

Author keywords

Botrytis cinerea; Gene isolation; Single oligonucleotide nested PCR; Thermal asymmetric interlaced PCR

Indexed keywords

FUNGAL DNA; PRIMER DNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; BOTRYTIS CINEREA; CONTROLLED STUDY; DNA ISOLATION; DNA SEQUENCE; DNA TEMPLATE; EXPRESSED SEQUENCE TAG; GENE AMPLIFICATION; GENE ISOLATION; METHODOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SINGLE OLIGONUCLEOTIDE NESTED POLYMERASE CHAIN REACTION;

BASE SEQUENCE; BLOTTING, SOUTHERN; BOTRYTIS; DNA PRIMERS; DNA, FUNGAL; EXPRESSED SEQUENCE TAGS; GENES, FUNGAL; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, NUCLEIC ACID;

BOTRYOTINIA FUCKELIANA; BOTRYTIS; FUNGI;

EID: 7744234354     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-004-0524-6     Document Type: Article

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