Plant enzymes but not Agrobacterium VirD2 mediate T-DNA ligation in vitro

Molecular and Cellular Biology

Volumn 20, Issue 17, 2000, Pages 6317-6322

  Ziemienowlcz, A ^a   Tinland, B ^a   Bryant, J ^a   Gloeckler, V ^a   Hohn, B ^a  

^a UNIVERSITY OF GDA SK   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; OLIGONUCLEOTIDE; PLANT EXTRACT; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; VIRULENCE FACTOR;

ARTICLE; BACTERIAL VIRULENCE; BACTERIOPHAGE T4; COVALENT BOND; DNA INTEGRATION; DNA TRANSFER; GENE TARGETING; GENOME; NONHUMAN; PLANT CELL; PLASMID; PRIORITY JOURNAL; RHIZOBIUM RADIOBACTER;

ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEINS; DNA LIGASES; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE TARGETING; MODELS, GENETIC; OLIGONUCLEOTIDES; PEAS; PLANT EXTRACTS; PLANT PROTEINS; PLANTS, TOXIC; RECOMBINATION, GENETIC; RHIZOBIUM RADIOBACTER; THYMINE NUCLEOTIDES; TIME FACTORS; TOBACCO; VIRULENCE FACTORS;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; PROKARYOTA;

EID: 0033842945     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.17.6317-6322.2000     Document Type: Article

References (37)

1. The integrase family of site-specific recombinases: Regional similarities and global diversity
2. Plant transformation: A pilus in Agrobacterium T-DNA transfer
3. Bacterial conjugation protein MobA mediates integration of complex DNA structures into plant cells
4. Agrobacterium tumefaciens T-complex transport apparatus: A paradigm for a new family of multifunctional transporters in eubacteria
5. A DNA polymerase from maize axes: Its purification and possible role
6. DNA ligase activity in pea seedlings (Pisum sativum L.): Development of a sensitive assay system and partial characterization of soluble and chromatin-bound DNA ligases
7. DNA ligase in pea (Pisum sativum L.) seedlings: Changes in activity during germination and effects of deoxyribo-nucleotides
8. Function of the Ti-plasmid Vir proteins: T-complex formation and transfer to the plant cell
9. Covalently bound VirD2 protein of Agrobacterium tumefaciens protects the T-DNA from exonucleolytic degradation
10. Pilus assembly by Agrobacterium T-DNA transfer genes
11. Illegitimate recombination in plants: A model for T-DNA integration
12. Lessons in gene transfer to plants by a gifted microbe
13. VirD proteins of Agrobacterium tumefaciens are required for the formation of a covalent DNA-protein complex at the 5' terminus of T-strand molecules
14. New views of the biochemistry of eukaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase α
15. Agrobacterium T-strand production in vitro: Sequence-specific cleavage and 5' protection of single-stranded DNA templates by purified VirD2 protein
16. Nucleic acid transport in plant-pathogen interactions
17. DNA polymerases from plant cells
18. Integration of Agrobacterium T-DNA into a tobacco chromosome: Possible involvement of DNA homology between T-DNA and plant DNA
19. T-DNA integration: A mode of illegitimate recombination in plants
20. High-frequency illegitimate integration of transfected DNA at preintegrated target sites in a mammalian genome
21. DNA ligases
22. Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation
23. Site-specific cleavage and joining of single-stranded DNA by VirD2 protein of Agrobacterium tumefaciens Ti plasmids: Analogy to bacterial conjugation
24. Gene targeting in plants
25. Role of virulence proteins of Agrobacterium in the plant
26. Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells
27. Protein complexes binding to cis elements of the plant histone gene promoters: Multiplicity, phosphorylation and cell cycle alteration
28. Agrobacterium-plant cell DNA transport: Have virulence proteins, will travel
29. The integration of T-DNA into plant genomes
30. Recombination between prokaryotic and eukaryotic DNA: Integration of Agrobacterium tumefaciens T-DNA into the plant genome
31. The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T-DNA into the plant genome
32. Mammalian DNA ligases
33. VirD2 protein of Agrobacterium tumefaciens very tightly linked to the 5' end of T-strand DNA
34. Biochemical and functional comparison of DNA polymerases α, δ and ε from calf thymus
35. Association of the VirD2 protein with the 5' end of T strands in Agrobacterium tumefaciens
36. The Agrobacterium DNA transfer complex
37. Assembly of the VirB transport complex for DNA transfer from Agrobacterium tumefaciens to plant cells