An inverse PCR technique to rapidly isolate the flanking DNA of Dictyostelium insertion mutants

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 26, Issue 3, 2004, Pages 221-224

  Keim, Melanie ^b   Williams, Robin S B ^b   Harwood, Adrian J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b NONE

Author keywords

Dictyostelium; Inverse PCR; Plasmid rescue; REMI mutagenesis

Indexed keywords

CONTAMINATION; DNA; ENZYME KINETICS; ESCHERICHIA COLI; GENES; MUTAGENESIS;

ENZYME MEDIATED INTEGRATION; GENOMIC INTEGRATION; INSERTION SITE; PLASMIDS;

MOLECULAR BIOLOGY;

DNA; GENOMIC DNA; PLASMID DNA; RESTRICTION ENDONUCLEASE; PRIMER DNA; PROTOZOAL DNA;

ARTICLE; DICTYOSTELIUM; DNA FLANKING REGION; DNA ISOLATION; DNA SEQUENCE; ESCHERICHIA COLI; GENE INSERTION; GENE SEQUENCE; GENOME; INVERSE POLYMERASE CHAIN REACTION; MOLECULAR CLONING; MUTANT; NONHUMAN; PLASMID; SOUTHERN BLOTTING; ANIMAL; CHEMISTRY; GENE EXPRESSION REGULATION; GENETIC TRANSFORMATION; GENETICS; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TRANSPOSON;

DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM; ESCHERICHIA COLI;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DICTYOSTELIUM; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; DNA RESTRICTION ENZYMES; DNA TRANSPOSABLE ELEMENTS; DNA, PROTOZOAN; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PLASMIDS; POLYMERASE CHAIN REACTION; TRANSFORMATION, GENETIC;

EID: 16544378816     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:26:3:221     Document Type: Article

References (3)

1. Kuspa, A. and Loomis, W. F. (1992) Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc. Natl. Acad. Sci. USA 89, 8803-8807.
2. Long, D., Martin, M., Sundberg, E., Swinburne, J., Puangsomlee, P., and Coupland, G. (1993) The maize transposable element system Ac/Ds as a mutagen in Arabidopsis: identification of an albino mutation induced by Ds insertion. Proc. Natl. Acad. Sci. USA 90, 10,370-10,374.
3. Hard, D. L. and Ochman, H. (1996) Inverse polymerase chain reaction. In Methods in Molecular Biology, vol. 58: Basic DNA and RNA Protocols, Harwood, A. J., ed. Humana, Totowa, NJ, pp. 293-301.