Transgene integration in aspen: Structures of integration sites and mechanism of T-DNA integration

Plant Journal

Volumn 31, Issue 4, 2002, Pages 543-551

  Kumar, Sandeep ^a   Fladung, Matthias ^a  

^a Federal Research Centre for Forestry and Forest Products   (Germany)

Author keywords

Filler DNA; Illegitimate recombination; T DNA junctions; Transgenic trees

Indexed keywords

DNA; GENES; PROTEINS;

GENOMIC SEQUENCES;

PLANTS (BOTANY);

BASE SEQUENCE; DNA, BACTERIAL; DNA, PLANT; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PLANTS, GENETICALLY MODIFIED; SALICACEAE; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSFORMATION, GENETIC; TRANSGENES;

EMBRYOPHYTA;

EID: 0036689386     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2002.01368.x     Document Type: Article

References (35)

1. Recovery of Agrobacterium tumefaciens T-DNA molecules from whole plants early after transfer
2. Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein
3. The DNA sequences of T-DNA junctions suggest that complex DNA loci are formed by recombination process resembling T-DNA integration
4. T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs
5. Gene stability in transgenic aspen (Populus). I. Flanking DNA sequences and T-DNA structure
6. Genetic transformation of Populus genotypes with different chimeric gene constructs: Transformation efficiency and molecular analysis
7. Transposition of the maize transposable element Ac in transgenic aspen and its usability as a gene tagging system
8. Agrobacterium and plant genes involved in T-DNA transfer and integration
9. Integration of Agrobacterium tumefaciens transfer DNA (T-DNA) involves rearrangements of target plant DNA
10. Illegitimate recombination in plants: A model for T-DNA integration
11. Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions
12. Transgene repeats in aspen: Molecular characterization suggests simultaneous integration of independent T-DNAs into receptive hotspots in host genome
13. Gene stability in transgenic aspen (Populus). II. Molecular characterization of variable expression of transgene in wild and hybrid aspen
14. Gene targeting in plants
15. A chromosomal paracentric inversion associated with T-DNA integration in Arabidopsis
16. Integration of Agrobacterium T-DNA into a tobacco chromosome: Possible involvement of DNA homology between T-DNA and plant DNA
17. T-DNA integration: A mode of illegitimate recombination in plants
18. Palindromic sequences and A+T-rich DNA elements promote illegitimate recombination in Nicotiana tabacum
19. Major chromosomal rearrangements induced by T-DNA transformation in Arabidopsis
20. Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation
21. Mechanisms of DNA double-strand break repair and their potential to induce chromosomal aberrations
22. Repair of genomic double-strand breaks in somatic plant cells by one-sided invasion of homologous sequences
23. Abortive gap repair: The underlying mechanism for Ds elements formation
24. Compositional compartmentalization and compositional patterns in nuclear genome of plants
25. Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells
26. The structures of integration sites in transgenic rice
27. T-DNA-associated duplication/translocations in Arabidopsis. Implications for mutant analysis and functional genomics
28. The integration of T-DNA into plant genomes
29. Recombination between prokaryotic and eukaryotic DNA: Integration of Agrobacterium tumefaciens T-DNA into the plant genome
30. Agrobacterium tumefaciens transfers single-stranded transfer DNA (T-DNA) into the plant cell nucleus
31. Basic processes underlying Agrobacterium-mediated DNA transfer to plant cells
32. Transfer and function of T-DNA genes from Agrobacterium Ti and Ri plasmids in plants
33. Plant enzymes but not Agrobacterium VirD2 mediate T-DNA ligation in vitro
34. Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells
35. The transfer of DNA from Agrobacterium tumefaciens into plants: A feast of fundamental insights