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Volumn 132, Issue 26, 2010, Pages 9007-9013

Dueling post-translational modifications trigger folding and unfolding of a β-hairpin peptide

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION; AMINO ACIDS; GENE EXPRESSION; PEPTIDES; PHOSPHORYLATION; SOLUTIONS;

EID: 77954251404     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja101079z     Document Type: Article
Times cited : (17)

References (71)
  • 33
    • 84925570147 scopus 로고    scopus 로고
    • It should be noted that using these methods to calculate the fraction folded assumes a two-state system between the folded and unfolded states. The folding data from Hα shifts and Gly splitting, which provide information about the folding of the strands and the turn, respectively, are in good agreement, supporting a two-state system. Nonetheless, thermal denaturation studies indicate that folding and unfolding are not highly cooperative, since a sharp transition from folded to unfolded is not observed.
    • It should be noted that using these methods to calculate the fraction folded assumes a two-state system between the folded and unfolded states. The folding data from Hα shifts and Gly splitting, which provide information about the folding of the strands and the turn, respectively, are in good agreement, supporting a two-state system. Nonetheless, thermal denaturation studies indicate that folding and unfolding are not highly cooperative, since a sharp transition from folded to unfolded is not observed.
  • 34
    • 84925567016 scopus 로고    scopus 로고
    • note
    • For comparison, we found no significant difference in the Hα chemical shifts of the unfolded peptides corresponding to residues 1-7, indicating that the modifications did not measurably affect the unfolded state. See the Supporting Information.
  • 35
    • 84925560555 scopus 로고    scopus 로고
    • note
    • NOESY experiments gave a larger amount of cross-peaks than ROESY experiments, but the ROEs that were observed were consistent with the corresponding NOEs observed for each peptide.
  • 42
    • 84925567015 scopus 로고    scopus 로고
    • note
    • It should be noted that phosphorylation also can stabilize the structure as well, making this post-translational modification very context dependent. See refs 13-15, 43, and 44 for examples.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.