Continuous fluorescence-based method for assessing Dicer cleavage efficiency reveals 3′ overhang nucleotide preference

BioTechniques

Volumn 48, Issue 4, 2010, Pages 303-311

  DiNitto, Jonathan P ^a   Wang, Leyu ^a,b   Wu, Joe C ^a  

^a PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

^b Adnexus Therapeutics   (United States)

Author keywords

Dicer; Gene silencing; Nucleic acid; RISC; RNAi; siRNA

Indexed keywords

CYANINE DYE 5; DICER; DOUBLE STRANDED RNA; FLUORESCENT DYE; IOWA BLACK RQ; PURINE NUCLEOTIDE; RNA INDUCED SILENCING COMPLEX; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; HUMAN; PROTEIN PROCESSING; QUANTITATIVE ANALYSIS; RNA CLEAVAGE; RNA SEQUENCE;

BASE SEQUENCE; BINDING, COMPETITIVE; CARBOCYANINES; FLUORESCENT DYES; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; RIBONUCLEASE III; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

EID: 77954073941     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113402     Document Type: Article

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