The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus

Protein Expression and Purification

Volumn 65, Issue 2, 2009, Pages 122-132

  Wasilko, David J ^a   Edward Lee, S ^a   Stutzman Engwall, Kim J ^a   Reitz, Beverly A ^a   Emmons, Thomas L ^a   Mathis, Karl J ^a   Bienkowski, Michael J ^a   Tomasselli, Alfredo G ^a   David Fischer, H ^a  

^a PFIZER INC   (United States)

Author keywords

Baculovirus infected insect cells (BIIC); Estimated MOI (eMOI); Protein expression; Soluble guanylate cyclase (sGC); Titerless infected cells preservation and scale up (TIPS)

Indexed keywords

CELL RECEPTOR; GUANYLATE CYCLASE; RECOMBINANT PROTEIN; SOLUBLE GUANYLYL CYCLASE;

ANIMAL; ARMYWORM; ARTICLE; BACULOVIRUS; BIOSYNTHESIS; CELL CULTURE; CHEMISTRY; CYTOLOGY; ENZYME ACTIVATION; GENETICS; HUMAN; METABOLISM; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; TIME; VIROLOGY; WESTERN BLOTTING;

ANIMALS; BACULOVIRIDAE; BLOTTING, WESTERN; CELLS, CULTURED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; GUANYLATE CYCLASE; HUMANS; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT PROTEINS; SPODOPTERA; TIME FACTORS;

HEMIPTERA; HEXAPODA;

EID: 61749088744     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.01.002     Document Type: Article

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