Redesigning of anti-c-Met single chain Fv antibody for the cytoplasmic folding and its structural analysis

Biotechnology and Bioengineering

Volumn 106, Issue 3, 2010, Pages 367-375

  Edwardraja, Selvakumar ^a   Neelamegam, Rameshkumar ^a   Ramadoss, Vijayaraj ^b   Venkatesan, Subramanian ^a   Lee, Sun Gu ^a  

^a Pusan National University   (South Korea)

^b CENTRAL LEATHER RESEARCH INSTITUTE   (India)

Author keywords

Antibody stability; CDR grafting; Humanization; Reducing cytoplasm; Single chain Fv (scFv)

Indexed keywords

COMPARATIVE ANALYSIS; DISULFIDE BONDS; E. COLI; HOMOLOGY MODELING; HUMANIZATION; MODEL SYSTEM; NATIVE FORMS; REDUCING CONDITIONS; SALT BRIDGES; SINGLE-CHAIN FV; SPECIFIC BINDING ACTIVITY; STRUCTURAL FEATURE; WILD TYPES;

ANTIGENS; CELLS; CYTOLOGY; ESCHERICHIA COLI; GRAFTING (CHEMICAL); STRUCTURAL ANALYSIS; SYSTEM STABILITY;

ANTIBODIES;

RECEPTOR ANTIBODY; SCATTER FACTOR RECEPTOR ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY ENGINEERING; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CODON; COMPLEMENTARITY DETERMINING REGION; CONSENSUS SEQUENCE; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; HYDROPHOBICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN FOLDING; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; WILD TYPE;

CYTOPLASM; ESCHERICHIA COLI; HUMANS; MODELS, MOLECULAR; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTO-ONCOGENE PROTEINS C-MET; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES;

ESCHERICHIA COLI;

EID: 77953567228     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22702     Document Type: Article

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