Single residue within the antigen translocation complex TAP controls the epitope repertoire by stabilizing a receptive conformation

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 20, 2010, Pages 9135-9140

  Baldauf, Christoph ^a   Schrodt, Susanne ^a   Herget, Meike ^a,b   Koch, Joachim ^a,c   Tampé, Robert ^a  

^a GOETHE UNIVERSITY   (Germany)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c INSTITUTE FOR TUMOR BIOLOGY AND EXPERIMENTAL THERAPY   (Germany)

Author keywords

ATP binding cassette transporter; Cysteine scanning mutagenesis; Membrane proteins; Molecular recognition; Substrate specificity

Indexed keywords

CYSTEINE; EPITOPE; THIOL; TRANSPORTER ASSOCIATED WITH ANTIGEN PROCESSING 1; TRANSPORTER ASSOCIATED WITH ANTIGEN PROCESSING 2; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME SPECIFICITY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN SYNTHESIS;

ATP-BINDING CASSETTE TRANSPORTERS; CYSTEINE; EPITOPES, T-LYMPHOCYTE; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; IMMUNOBLOTTING; MODELS, MOLECULAR; MUTAGENESIS; PROTEIN BINDING; PROTEIN CONFORMATION;

EID: 77952709552     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1001308107     Document Type: Article

References (33)

1. Lehner PJ, Cresswell P (2004) Recent developments in MHC-class-I-mediated antigen presentation. Curr Opin Immunol 16:82-89. (Pubitemid 38198121)
2. Rock KL, et al. (1994) Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules. Cell 78:761-771.
3. Yewdell JW, Haeryfar SM (2005) Understanding presentation of viral antigens to CD8+ T cells in vivo: The key to rational vaccine design. Annu Rev Immunol 23:651-682.
4. Wearsch PA, Cresswell P (2008) The quality control of MHC class I peptide loading. Curr Opin Cell Biol 20:624-631.
5. Abele R, Tampé R (2009) Peptide trafficking and translocation across membranes in cellular signaling and self-defense strategies. Curr Opin Cell Biol 21:508-515.
6. Schmitt L, Tampé R (2002) Structure and mechanism of ABC transporters. Curr Opin Struct Biol 12:754-760.
7. Koch J, Guntrum R, Heintke S, Kyritsis C, Tampé R (2004) Functional dissection of the transmembrane domains of the transporter associated with antigen processing (TAP). J Biol Chem 279:10142-10147. (Pubitemid 38372619)
8. van Endert PM, et al. (1994) A sequential model for peptide binding and transport by the transporters associated with antigen processing. Immunity 1:491-500. (Pubitemid 2142273)
9. Meyer TH, van Endert PM, Uebel S, Ehring B, Tampé R (1994) Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells. FEBS Lett 351:443-447.
10. Nijenhuis M, Hämmerling GJ (1996) Multiple regions of the transporter associated with antigen processing (TAP) contribute to its peptide binding site. J Immunol 157:5467-5477. (Pubitemid 126448967)
11. Nijenhuis M, et al. (1996) Identification of a contact region for peptide on the TAP1 chain of the transporter associated with antigen processing. J Immunol 156:2186-2195. (Pubitemid 26087449)
12. van Endert PM, et al. (1995) The peptide-binding motif for the human transporter associated with antigen processing. J Exp Med 182:1883-1895.
13. Uebel S, et al. (1997) Recognition principle of the TAP transporter disclosed by combinatorial peptide libraries. Proc Natl Acad Sci USA 94:8976-8981. (Pubitemid 27357767)
14. Peters B, Bulik S, Tampé R, Van Endert PM, Holzhutter HG (2003) Identifying MHC class I epitopes by predicting the TAP transport efficiency of epitope precursors. J Immunol 171:1741-1749. (Pubitemid 36966457)
15. Schrodt S, Koch J, Tampé R (2006) Membrane topology of the transporter associated with antigen processing (TAP1) within an assembled functional peptide-loading complex. J Biol Chem 281:6455-6462. (Pubitemid 43847578)
16. Abele R, Tampé R (2004) The ABCs of immunology: Structure and function of TAP, the transporter associated with antigen processing. Physiology (Bethesda) 19:216-224.
17. Procko E, O'Mara ML, Bennett WF, Tieleman DP, Gaudet R (2009) The mechanism of ABC transporters: General lessons from structural and functional studies of an antigenic peptide transporter. FASEB J 23:1287-1302.
18. Heemels MT, Ploegh HL (1994) Substrate specificity of allelic variants of the TAP peptide transporter. Immunity 1:775-784.
19. Heintke S, et al. (2003) Functional cysteine-less subunits of the transporter associated with antigen processing (TAP1 and TAP2) by de novo gene assembly. FEBS Lett 533:42-46.
20. Nie Y, Sabetfard FE, Kaback HR (2008) The Cys154->Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J Mol Biol 379:695-703.
21. Menick DR, Sarkar HK, Poonian MS, Kaback HR (1985) Cys154 is important for lac permease activity in Escherichia coli. Biochem Biophys Res Commun 132:162-170. (Pubitemid 16196125)
22. Cui G, Zhang ZR, O'Brien AR, Song B, McCarty NA (2008) Mutations at arginine 352 alter the pore architecture of CFTR. J Membr Biol 222:91-106.
23. Ward A, Reyes CL, Yu J, Roth CB, Chang G (2007) Flexibility in the ABC transporter MsbA: Alternating access with a twist. Proc Natl Acad Sci USA 104:19005-19010.
24. Smirnova I, et al. (2007) Sugar binding induces an outward facing conformation of LacY. Proc Natl Acad Sci USA 104:16504-16509. (Pubitemid 350211048)
25. Dawson RJ, Locher KP (2006) Structure of a bacterial multidrug ABC transporter. Nature 443:180-185.
26. Ernst R, et al. (2008) A mutation of the H-loop selectively affects rhodamine transport by the yeast multidrug ABC transporter Pdr5. Proc Natl Acad Sci USA 105:5069-5074. (Pubitemid 351738511)
27. Gorbulev S, Abele R, Tampé R (2001) Allosteric crosstalk between peptide-binding, transport, and ATP hydrolysis of the ABC transporter TAP. Proc Natl Acad Sci USA 98:3732-3737. (Pubitemid 32249840)
28. Herget M, et al. (2009) Purification and reconstitution of the antigen transport complex TAP: A prerequisite for determination of peptide stoichiometry and ATP hydrolysis. J Biol Chem 284:33740-33749.
29. Oancea G, et al. (2009) Structural arrangement of the transmission interface in the antigen ABC transport complex TAP. Proc Natl Acad Sci USA 106:5551-5556.
30. Armandola EA, et al. (1996) A point mutation in the human transporter associated with antigen processing (TAP2) alters the peptide transport specificity. Eur J Immunol 26:1748-1755.
31. Momburg F, Armandola EA, Post M, Hämmerling GJ (1996) Residues in TAP2 peptide transporters controlling substrate specificity. J Immunol 156:1756-1763. (Pubitemid 26069307)
32. Momburg F, et al. (1994) Selectivity of MHC-encoded peptide transporters from human, mouse and rat. Nature 367:648-651. (Pubitemid 24067707)
33. Herget M, et al. (2007) Mechanism of substrate sensing and signal transmission within an ABC transporter: use of a Trojan horse strategy. J Biol Chem 282:3871-3880. (Pubitemid 47084466)